Construct: ORF ccsbBroad304_14445
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011572.1_s304c1, BRDN0000409362
- Derived from:
- ccsbBroadEn_14445
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- CNPY4 (245812)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_14445
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 245812 | CNPY4 | canopy FGF signaling regula... | NM_152755.2 | 99.5% | 88.3% | 656_658delAGGinsNN |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 765
- ORF length:
- 699
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggg acctgtgcgg ttgggaatat tgcttttcct ttttttggcc gtgcacgagg 121 cttgggctgg gatgttgaag gaggaggacg atgacacaga acgcttgccc agcaaatgcg 181 aagtgtgtaa gctgctgagc acagagctac aggcggaact gagtcgcacc ggtcgatctc 241 gagaggtgct ggagctgggg caggtgctgg atacaggcaa gaggaagaga cacgtgcctt 301 acagcgtttc agagacaagg ctggaagagg ccttagagaa tttatgtgag cggatcctgg 361 actatagtgt tcacgctgag cgcaagggct cactgagata tgccaagggt cagagtcaga 421 ccatggcaac actgaaaggc ctagtgcaga agggggtgaa ggtggatctg gggatccctc 481 tggagctttg ggatgagccc agcgtggagg tcacatacct caagaagcag tgtgagacca 541 tgttggagga gtttgaagac attgtgggag actggtactt ccaccatcag gagcagcccc 601 tacaaaattt tctctgtgaa ggtcatgtgc tcccagctgc tgaaactgca tgtctacagg 661 aaacttggac tggaaaggag atcacagatg gggaagagaa aacagaaggg gaggaagagc 721 nnaggaggag gaggaagagg aggaagagga agggggagac aagatgacca agacaggaag 781 ccaccccaaa cttgaccgag aagatcttta cccaactttc ttgtacaaag tggttggtaa 841 gcctatccct aaccctctcc tcggtctcga ttctacgtag taatgagcta gcgctaaccg 901 gtggcgcgtt aagtcgacaa tcaacctctg gatta