Construct: ORF ccsbBroad304_14483
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013710.1_s304c1, BRDN0000405163
- Derived from:
- ccsbBroadEn_14483
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to frame shift)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- C12orf42 (374470)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_14483
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 374470 | C12orf42 | chromosome 12 open reading ... | NM_001099336.2 | 99.8% | 98% | 33A>C;1066delA |
| 2 | human | 374470 | C12orf42 | chromosome 12 open reading ... | NM_198521.4 | 99.8% | 98% | 33A>C;1066delA |
| 3 | human | 374470 | C12orf42 | chromosome 12 open reading ... | XM_011538292.2 | 99.8% | 98% | 33A>C;1066delA |
| 4 | human | 374470 | C12orf42 | chromosome 12 open reading ... | XM_011538293.2 | 99.8% | 98% | 33A>C;1066delA |
| 5 | human | 374470 | C12orf42 | chromosome 12 open reading ... | XM_017019276.1 | 99.8% | 98% | 33A>C;1066delA |
| 6 | human | 374470 | C12orf42 | chromosome 12 open reading ... | XM_011538291.1 | 94.8% | 93.1% | 1_57del;90A>C;1123delA |
| 7 | human | 374470 | C12orf42 | chromosome 12 open reading ... | XM_011538295.1 | 92.9% | 91.1% | 0_1ins72;2_3insGCA;991delA |
| 8 | human | 374470 | C12orf42 | chromosome 12 open reading ... | XM_011538294.2 | 88.7% | 87.1% | (many diffs) |
| 9 | human | 374470 | C12orf42 | chromosome 12 open reading ... | XM_011538297.2 | 87.8% | 85.3% | (many diffs) |
| 10 | human | 374470 | C12orf42 | chromosome 12 open reading ... | XM_011538300.1 | 87.8% | 85.3% | (many diffs) |
| 11 | human | 374470 | C12orf42 | chromosome 12 open reading ... | XM_017019277.1 | 87.8% | 85.3% | (many diffs) |
| 12 | human | 374470 | C12orf42 | chromosome 12 open reading ... | XM_017019278.1 | 87.8% | 85.3% | (many diffs) |
| 13 | human | 374470 | C12orf42 | chromosome 12 open reading ... | XM_024448971.1 | 87.8% | 85.3% | (many diffs) |
| 14 | human | 374470 | C12orf42 | chromosome 12 open reading ... | XM_006719377.3 | 81.2% | 79.7% | 0_1ins201;865delA |
| 15 | human | 374470 | C12orf42 | chromosome 12 open reading ... | XM_011538305.1 | 81.2% | 79.7% | 0_1ins201;865delA |
| 16 | human | 374470 | C12orf42 | chromosome 12 open reading ... | XM_017019279.1 | 81.2% | 79.7% | 0_1ins201;865delA |
| 17 | human | 374470 | C12orf42 | chromosome 12 open reading ... | XM_017019280.1 | 81.2% | 79.7% | 0_1ins201;865delA |
| 18 | human | 374470 | C12orf42 | chromosome 12 open reading ... | NM_001278419.2 | 73.5% | 72% | 0_1ins285;781delA |
| 19 | human | 374470 | C12orf42 | chromosome 12 open reading ... | NM_001278420.2 | 73.5% | 72% | 0_1ins285;781delA |
| 20 | human | 374470 | C12orf42 | chromosome 12 open reading ... | XM_011538306.1 | 62% | 60.5% | (many diffs) |
| 21 | human | 374470 | C12orf42 | chromosome 12 open reading ... | XM_011538315.2 | 35% | 23.2% | (many diffs) |
| 22 | human | 374470 | C12orf42 | chromosome 12 open reading ... | XM_017019281.1 | 34.5% | 25% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1149
- ORF length:
- 1080
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gtctacagtg atatgtatga aacaaaggga cgaagaattc ttgctaacca 121 tcagaccttt tgcaaacagg atgcagaaat ccccttgcta tattcccatt gtgagcagtg 181 ccaccctgtg ggatagaagc acacccagtg caaagcacat cccttgttat gaaagaactt 241 cagtaccctg ctccagattc attaatcaca tgaagaattt ctctgaatct cctaaatttc 301 gtagtctaca ctttctgaat tttccagtat ttccagaaag gactcaaaat tcaatggcgt 361 gtaaaagact acttcatact tgccagtaca tagtccccag gtgttctgta agcacagttt 421 cttttgatga agaaagctat gaagaattcc gttcctctcc agcaccatcc agtgaaactg 481 atgaggcccc attgattttt actgccagag gagaaactga ggagagagcc agaggagcac 541 ccaagcaggc ttggaacagt tcatttttgg aacaactggt taaaaagcct aactgggcac 601 actcagtaaa tcctgttcac ctggaggctc agggcataca catcagtaga cacacaagac 661 ctaagggcca gcccttgagc agtcccaaga aaaattctgg ttctgccgcc agaccttcca 721 ctgccatcgg cctctgcagg aggagccaga cgcccggcgc tctgcagagc accggcccga 781 gtaacacaga gctcgagccg gaggagagga tggcagtccc agcaggcgct caggcacacc 841 ccgacgacat ccaaagcaga ctcctgggcg cgtccggaaa tcccgtcgga aaaggcgcgg 901 ttgccatggc gccggagatg ctccccaagc atcctcatac cccgcgggac aggaggcctc 961 aggcggacac ctccctccat ggcaatctgg caggagcgcc ccttcctctg ctggccggtg 1021 cttccaccca tttcccctcc aagaggttaa taaaggtttg ctcctcagca cccccccgcc 1081 caacccggcg tttccatacg gtttgttcac aggccctttc taggccggtg gtgatgctca 1141 cttacatttg ccaactttct tgtacaaagt ggttggtaag cctatcccta accctctcct 1201 cggtctcgat tctacgtagt aatgagctag cgctaaccgg tggcgcgtta agtcgacaat 1261 caacctctgg atta