Construct: ORF ccsbBroad304_14516
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015716.1_s304c1, BRDN0000460197
- Derived from:
- ccsbBroadEn_14516
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- RSKR (124923)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_14516
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 124923 | RSKR | ribosomal protein S6 kinase... | NM_001174103.2 | 24.1% | 2.4% | 1_933del;961_962insC |
| 2 | mouse | 216976 | BC030499 | cDNA sequence BC030499 | XM_006532970.3 | 38% | 3.1% | (many diffs) |
| 3 | mouse | 216976 | BC030499 | cDNA sequence BC030499 | XM_006532969.3 | 31.3% | 2.5% | (many diffs) |
| 4 | mouse | 216976 | BC030499 | cDNA sequence BC030499 | XM_006532968.3 | 28.9% | 2.3% | (many diffs) |
| 5 | mouse | 216976 | BC030499 | cDNA sequence BC030499 | XM_006532967.3 | 24% | 1.9% | (many diffs) |
| 6 | mouse | 216976 | BC030499 | cDNA sequence BC030499 | XM_006532966.3 | 22.2% | 1.8% | (many diffs) |
| 7 | mouse | 216976 | BC030499 | cDNA sequence BC030499 | NM_001287206.1 | 21% | 1.7% | (many diffs) |
| 8 | mouse | 216976 | BC030499 | cDNA sequence BC030499 | XM_006532965.3 | 21% | 1.7% | (many diffs) |
| 9 | mouse | 216976 | BC030499 | cDNA sequence BC030499 | XR_388407.3 | 18% | (many diffs) | |
| 10 | mouse | 216976 | BC030499 | cDNA sequence BC030499 | XR_388406.3 | 10.9% | (many diffs) | |
| 11 | mouse | 216976 | BC030499 | cDNA sequence BC030499 | XR_388408.3 | 7.6% | (many diffs) | |
| 12 | mouse | 216976 | BC030499 | cDNA sequence BC030499 | XR_001779956.1 | 6.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 99
- ORF length:
- 30
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gttggcaagt gtgacccaca gtgactcctg agatcccagc ttctcttaac 121 cagggcctct cactcctgct ccatgagctc ttatgccaga accccctcca tcgtctacgt 181 tatctgcatc acttccaggt ccaccctttc tttcggggtg tggccttcga cccagagctc 241 ctacagaagc agccagtgaa ctttgtcacg gagacacaag ctacccagcc cagttcagcg 301 gagaccatgc cctttgacga ctttgactgt gatctggagt ccttcttgct ctaccctatc 361 cctgctttgc caactttctt gtacaaagtg gttggtaagc ctatccctaa ccctctcctc 421 ggtctcgatt ctacgtagta atgagctagc gctaaccggt ggcgcgttaa gtcgacaatc 481 aacctctgga tta