Construct: ORF ccsbBroad304_14532
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014666.2_s304c1, BRDN0000459751
- Derived from:
- ccsbBroadEn_14532
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GRK2 (156)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_14532
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 156 | GRK2 | G protein-coupled receptor ... | NM_001619.5 | 99.9% | 100% | 96C>A |
| 2 | human | 156 | GRK2 | G protein-coupled receptor ... | XM_024448376.1 | 95.9% | 93.8% | (many diffs) |
| 3 | human | 156 | GRK2 | G protein-coupled receptor ... | XM_011544773.1 | 95.2% | 94.9% | (many diffs) |
| 4 | human | 156 | GRK2 | G protein-coupled receptor ... | XR_001747779.1 | 57.8% | (many diffs) | |
| 5 | mouse | 110355 | Grk2 | G protein-coupled receptor ... | NM_001290818.1 | 90.2% | 98.8% | (many diffs) |
| 6 | mouse | 110355 | Grk2 | G protein-coupled receptor ... | NM_130863.2 | 84.3% | 92.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2133
- ORF length:
- 2067
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc ggacctggag gcggtgctgg ccgacgtgag ctacctgatg gccatggaga 121 agagcaaggc cacgccggcc gcgcgcgcca gcaagaagat actgctgccc gagcccagca 181 tccgcagtgt catgcagaag tacctggagg accggggcga ggtgaccttt gagaagatct 241 tttcccagaa gctggggtac ctgctcttcc gagacttctg cctgaaccac ctggaggagg 301 ccaggccctt ggtggaattc tatgaggaga tcaagaagta cgagaagctg gagacggagg 361 aggagcgtgt ggcccgcagc cgggagatct tcgactcata catcatgaag gagctgctgg 421 cctgctcgca tcccttctcg aagagtgcca ctgagcatgt ccaaggccac ctggggaaga 481 agcaggtgcc tccggatctc ttccagccat acatcgaaga gatttgtcaa aacctccgag 541 gggacgtgtt ccagaaattc attgagagcg ataagttcac acggttttgc cagtggaaga 601 atgtggagct caacatccac ctgaccatga atgacttcag cgtgcatcgc atcattgggc 661 gcgggggctt tggcgaggtc tatgggtgcc ggaaggctga cacaggcaag atgtacgcca 721 tgaagtgcct ggacaaaaag cgcatcaaga tgaagcaggg ggagaccctg gccctgaacg 781 agcgcatcat gctctcgctc gtcagcactg gggactgccc attcattgtc tgcatgtcat 841 acgcgttcca cacgccagac aagctcagct tcatcctgga cctcatgaac ggtggggacc 901 tgcactacca cctctcccag cacggggtct tctcagaggc tgacatgcgc ttctatgcgg 961 ccgagatcat cctgggcctg gagcacatgc acaaccgctt cgtggtctac cgggacctga 1021 agccagccaa catccttctg gacgagcatg gccacgtgcg gatctcggac ctgggcctgg 1081 cctgtgactt ctccaagaag aagccccatg ccagcgtggg cacccacggg tacatggctc 1141 cggaggtcct gcagaagggc gtggcctacg acagcagtgc cgactggttc tctctggggt 1201 gcatgctctt caagttgctg cgggggcaca gccccttccg gcagcacaag accaaagaca 1261 agcatgagat cgaccgcatg acgctgacga tggccgtgga gctgcccgac tccttctccc 1321 ctgaactacg ctccctgctg gaggggttgc tgcagaggga tgtcaaccgg agattgggct 1381 gcctgggccg aggggctcag gaggtgaaag agagcccctt tttccgctcc ctggactggc 1441 agatggtctt cttgcagaag taccctcccc cgctgatccc cccacgaggg gaggtgaacg 1501 cggccgacgc cttcgacatt ggctccttcg atgaggagga cacaaaagga atcaagttac 1561 tggacagtga tcaggagctc taccgcaact tccccctcac catctcggag cggtggcagc 1621 aggaggtggc agagactgtc ttcgacacca tcaacgctga gacagaccgg ctggaggctc 1681 gcaagaaagc caagaacaag cagctgggcc atgaggaaga ctacgccctg ggcaaggact 1741 gcatcatgca tggctacatg tccaagatgg gcaacccctt cctgacccag tggcagcggc 1801 ggtacttcta cctgttcccc aaccgcctcg agtggcgggg cgagggcgag gccccgcaga 1861 gcctgctgac catggaggag atccagtcgg tggaggagac gcagatcaag gagcgcaagt 1921 gcctgctcct caagatccgc ggtgggaaac agttcatttt gcagtgcgat agcgaccctg 1981 agctggtgca gtggaagaag gagctgcgcg acgcctaccg cgaggcccag cagctggtgc 2041 agcgggtgcc caagatgaag aacaagccgc gctcgcccgt ggtggagctg agcaaggtgc 2101 cgctggtcca gcgcggcagt gccaacggcc tctgcccaac tttcttgtac aaagtggttg 2161 gtaagcctat ccctaaccct ctcctcggtc tcgattctac gtagtaatga gctagcgcta 2221 accggtggcg cgttaagtcg acaatcaacc tctggatta