Construct: ORF ccsbBroad304_14532

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF014666.2_s304c1, BRDN0000459751
Derived from:
ccsbBroadEn_14532
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
GRK2 (156)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_14532
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 156 GRK2 G protein-coupled receptor ... NM_001619.5 99.9% 100% 96C>A
2 human 156 GRK2 G protein-coupled receptor ... XM_024448376.1 95.9% 93.8% (many diffs)
3 human 156 GRK2 G protein-coupled receptor ... XM_011544773.1 95.2% 94.9% (many diffs)
4 human 156 GRK2 G protein-coupled receptor ... XR_001747779.1 57.8% (many diffs)
5 mouse 110355 Grk2 G protein-coupled receptor ... NM_001290818.1 90.2% 98.8% (many diffs)
6 mouse 110355 Grk2 G protein-coupled receptor ... NM_130863.2 84.3% 92.7% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2133
ORF length:
2067
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggc ggacctggag gcggtgctgg ccgacgtgag ctacctgatg gccatggaga
121agagcaaggc cacgccggcc gcgcgcgcca gcaagaagat actgctgccc gagcccagca
181tccgcagtgt catgcagaag tacctggagg accggggcga ggtgaccttt gagaagatct
241tttcccagaa gctggggtac ctgctcttcc gagacttctg cctgaaccac ctggaggagg
301ccaggccctt ggtggaattc tatgaggaga tcaagaagta cgagaagctg gagacggagg
361aggagcgtgt ggcccgcagc cgggagatct tcgactcata catcatgaag gagctgctgg
421cctgctcgca tcccttctcg aagagtgcca ctgagcatgt ccaaggccac ctggggaaga
481agcaggtgcc tccggatctc ttccagccat acatcgaaga gatttgtcaa aacctccgag
541gggacgtgtt ccagaaattc attgagagcg ataagttcac acggttttgc cagtggaaga
601atgtggagct caacatccac ctgaccatga atgacttcag cgtgcatcgc atcattgggc
661gcgggggctt tggcgaggtc tatgggtgcc ggaaggctga cacaggcaag atgtacgcca
721tgaagtgcct ggacaaaaag cgcatcaaga tgaagcaggg ggagaccctg gccctgaacg
781agcgcatcat gctctcgctc gtcagcactg gggactgccc attcattgtc tgcatgtcat
841acgcgttcca cacgccagac aagctcagct tcatcctgga cctcatgaac ggtggggacc
901tgcactacca cctctcccag cacggggtct tctcagaggc tgacatgcgc ttctatgcgg
961ccgagatcat cctgggcctg gagcacatgc acaaccgctt cgtggtctac cgggacctga
1021agccagccaa catccttctg gacgagcatg gccacgtgcg gatctcggac ctgggcctgg
1081cctgtgactt ctccaagaag aagccccatg ccagcgtggg cacccacggg tacatggctc
1141cggaggtcct gcagaagggc gtggcctacg acagcagtgc cgactggttc tctctggggt
1201gcatgctctt caagttgctg cgggggcaca gccccttccg gcagcacaag accaaagaca
1261agcatgagat cgaccgcatg acgctgacga tggccgtgga gctgcccgac tccttctccc
1321ctgaactacg ctccctgctg gaggggttgc tgcagaggga tgtcaaccgg agattgggct
1381gcctgggccg aggggctcag gaggtgaaag agagcccctt tttccgctcc ctggactggc
1441agatggtctt cttgcagaag taccctcccc cgctgatccc cccacgaggg gaggtgaacg
1501cggccgacgc cttcgacatt ggctccttcg atgaggagga cacaaaagga atcaagttac
1561tggacagtga tcaggagctc taccgcaact tccccctcac catctcggag cggtggcagc
1621aggaggtggc agagactgtc ttcgacacca tcaacgctga gacagaccgg ctggaggctc
1681gcaagaaagc caagaacaag cagctgggcc atgaggaaga ctacgccctg ggcaaggact
1741gcatcatgca tggctacatg tccaagatgg gcaacccctt cctgacccag tggcagcggc
1801ggtacttcta cctgttcccc aaccgcctcg agtggcgggg cgagggcgag gccccgcaga
1861gcctgctgac catggaggag atccagtcgg tggaggagac gcagatcaag gagcgcaagt
1921gcctgctcct caagatccgc ggtgggaaac agttcatttt gcagtgcgat agcgaccctg
1981agctggtgca gtggaagaag gagctgcgcg acgcctaccg cgaggcccag cagctggtgc
2041agcgggtgcc caagatgaag aacaagccgc gctcgcccgt ggtggagctg agcaaggtgc
2101cgctggtcca gcgcggcagt gccaacggcc tctgcccaac tttcttgtac aaagtggttg
2161gtaagcctat ccctaaccct ctcctcggtc tcgattctac gtagtaatga gctagcgcta
2221accggtggcg cgttaagtcg acaatcaacc tctggatta

Download FASTA (ORF) (Full)