Construct: ORF ccsbBroad304_14540
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019031.1_s304c1, BRDN0000459830
- Derived from:
- ccsbBroadEn_14540
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ALK (238)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_14540
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 238 | ALK | ALK receptor tyrosine kinase | NM_004304.5 | 100% | 100% | |
| 2 | human | 238 | ALK | ALK receptor tyrosine kinase | XR_001738688.2 | 54.8% | 1_930del;3670_3671ins74;4104_4105ins1612 | |
| 3 | human | 238 | ALK | ALK receptor tyrosine kinase | XM_024452778.1 | 41.4% | 41.4% | 0_1ins2847 |
| 4 | human | 238 | ALK | ALK receptor tyrosine kinase | NM_001353765.1 | 34% | 34% | 0_1ins3204 |
| 5 | human | 238 | ALK | ALK receptor tyrosine kinase | XM_024452779.1 | 34% | 34% | 0_1ins3204 |
| 6 | mouse | 11682 | Alk | anaplastic lymphoma kinase | NM_007439.2 | 83.6% | 87.1% | (many diffs) |
| 7 | mouse | 11682 | Alk | anaplastic lymphoma kinase | XR_001782029.1 | 53.9% | (many diffs) | |
| 8 | mouse | 11682 | Alk | anaplastic lymphoma kinase | XR_385265.3 | 43.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 4929
- ORF length:
- 4860
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gggagccatc gggctcctgt ggctcctgcc gctgctgctt tccacggcag 121 ctgtgggctc cgggatgggg accggccagc gcgcgggctc cccagctgcg gggccgccgc 181 tgcagccccg ggagccactc agctactcgc gcctgcagag gaagagtctg gcagttgact 241 tcgtggtgcc ctcgctcttc cgtgtctacg cccgggacct actgctgcca ccatcctcct 301 cggagctgaa ggctggcagg cccgaggccc gcggctcgct agctctggac tgcgccccgc 361 tgctcaggtt gctggggccg gcgccggggg tctcctggac cgccggttca ccagccccgg 421 cagaggcccg gacgctgtcc agggtgctga agggcggctc cgtgcgcaag ctccggcgtg 481 ccaagcagtt ggtgctggag ctgggcgagg aggcgatctt ggagggttgc gtcgggcccc 541 ccggggaggc ggctgtgggg ctgctccagt tcaatctcag cgagctgttc agttggtgga 601 ttcgccaagg cgaagggcga ctgaggatcc gcctgatgcc cgagaagaag gcgtcggaag 661 tgggcagaga gggaaggctg tccgcggcaa ttcgcgcctc ccagccccgc cttctcttcc 721 agatcttcgg gactggtcat agctccttgg aatcaccaac aaacatgcct tctccttctc 781 ctgattattt tacatggaat ctcacctgga taatgaaaga ctccttccct ttcctgtctc 841 atcgcagccg atatggtctg gagtgcagct ttgacttccc ctgtgagctg gagtattccc 901 ctccactgca tgacctcagg aaccagagct ggtcctggcg ccgcatcccc tccgaggagg 961 cctcccagat ggacttgctg gatgggcctg gggcagagcg ttctaaggag atgcccagag 1021 gctcctttct ccttctcaac acctcagctg actccaagca caccatcctg agtccgtgga 1081 tgaggagcag cagtgagcac tgcacactgg ccgtctcggt gcacaggcac ctgcagccct 1141 ctggaaggta cattgcccag ctgctgcccc acaacgaggc tgcaagagag atcctcctga 1201 tgcccactcc agggaagcat ggttggacag tgctccaggg aagaatcggg cgtccagaca 1261 acccatttcg agtggccctg gaatacatct ccagtggaaa ccgcagcttg tctgcagtgg 1321 acttctttgc cctgaagaac tgcagtgaag gaacatcccc aggctccaag atggccctgc 1381 agagctcctt cacttgttgg aatgggacag tcctccagct tgggcaggcc tgtgacttcc 1441 accaggactg tgcccaggga gaagatgaga gccagatgtg ccggaaactg cctgtgggtt 1501 tttactgcaa ctttgaagat ggcttctgtg gctggaccca aggcacactg tcaccccaca 1561 ctcctcaatg gcaggtcagg accctaaagg atgcccggtt ccaggaccac caagaccatg 1621 ctctattgct cagtaccact gatgtccccg cttctgaaag tgctacagtg accagtgcta 1681 cgtttcctgc accgatcaag agctctccat gtgagctccg aatgtcctgg ctcattcgtg 1741 gagtcttgag gggaaacgtg tccttggtgc tagtggagaa caaaaccggg aaggagcaag 1801 gcaggatggt ctggcatgtc gccgcctatg aaggcttgag cctgtggcag tggatggtgt 1861 tgcctctcct cgatgtgtct gacaggttct ggctgcagat ggtcgcatgg tggggacaag 1921 gatccagagc catcgtggct tttgacaata tctccatcag cctggactgc tacctcacca 1981 ttagcggaga ggacaagatc ctgcagaata cagcacccaa atcaagaaac ctgtttgaga 2041 gaaacccaaa caaggagctg aaacccgggg aaaattcacc aagacagacc cccatctttg 2101 accctacagt tcattggctg ttcaccacat gtggggccag cgggccccat ggccccaccc 2161 aggcacagtg caacaacgcc taccagaact ccaacctgag cgtggaggtg gggagcgagg 2221 gccccctgaa aggcatccag atctggaagg tgccagccac cgacacctac agcatctcgg 2281 gctacggagc tgctggcggg aaaggcggga agaacaccat gatgcggtcc cacggcgtgt 2341 ctgtgctggg catcttcaac ctggagaagg atgacatgct gtacatcctg gttgggcagc 2401 agggagagga cgcctgcccc agtacaaacc agttaatcca gaaagtctgc attggagaga 2461 acaatgtgat agaagaagaa atccgtgtga acagaagcgt gcatgagtgg gcaggaggcg 2521 gaggaggagg gggtggagcc acctacgtat ttaagatgaa ggatggagtg ccggtgcccc 2581 tgatcattgc agccggaggt ggtggcaggg cctacggggc caagacagac acgttccacc 2641 cagagagact ggagaataac tcctcggttc tagggctaaa cggcaattcc ggagccgcag 2701 gtggtggagg tggctggaat gataacactt ccttgctctg ggccggaaaa tctttgcagg 2761 agggtgccac cggaggacat tcctgccccc aggccatgaa gaagtggggg tgggagacaa 2821 gagggggttt cggagggggt ggaggggggt gctcctcagg tggaggaggc ggaggatata 2881 taggcggcaa tgcagcctca aacaatgacc ccgaaatgga tggggaagat ggggtttcct 2941 tcatcagtcc actgggcatc ctgtacaccc cagctttaaa agtgatggaa ggccacgggg 3001 aagtgaatat taagcattat ctaaactgca gtcactgtga ggtagacgaa tgtcacatgg 3061 accctgaaag ccacaaggtc atctgcttct gtgaccacgg gacggtgctg gctgaggatg 3121 gcgtctcctg cattgtgtca cccaccccgg agccacacct gccactctcg ctgatcctct 3181 ctgtggtgac ctctgccctc gtggccgccc tggtcctggc tttctccggc atcatgattg 3241 tgtaccgccg gaagcaccag gagctgcaag ccatgcagat ggagctgcag agccctgagt 3301 acaagctgag caagctccgc acctcgacca tcatgaccga ctacaacccc aactactgct 3361 ttgctggcaa gacctcctcc atcagtgacc tgaaggaggt gccgcggaaa aacatcaccc 3421 tcattcgggg tctgggccat ggcgcctttg gggaggtgta tgaaggccag gtgtccggaa 3481 tgcccaacga cccaagcccc ctgcaagtgg ctgtgaagac gctgcctgaa gtgtgctctg 3541 aacaggacga actggatttc ctcatggaag ccctgatcat cagcaaattc aaccaccaga 3601 acattgttcg ctgcattggg gtgagcctgc aatccctgcc ccggttcatc ctgctggagc 3661 tcatggcggg gggagacctc aagtccttcc tccgagagac ccgccctcgc ccgagccagc 3721 cctcctccct ggccatgctg gaccttctgc acgtggctcg ggacattgcc tgtggctgtc 3781 agtatttgga ggaaaaccac ttcatccacc gagacattgc tgccagaaac tgcctcttga 3841 cctgtccagg ccctggaaga gtggccaaga ttggagactt cgggatggcc cgagacatct 3901 acagggcgag ctactataga aagggaggct gtgccatgct gccagttaag tggatgcccc 3961 cagaggcctt catggaagga atattcactt ctaaaacaga cacatggtcc tttggagtgc 4021 tgctatggga aatcttttct cttggatata tgccataccc cagcaaaagc aaccaggaag 4081 ttctggagtt tgtcaccagt ggaggccgga tggacccacc caagaactgc cctgggcctg 4141 tataccggat aatgactcag tgctggcaac atcagcctga agacaggccc aactttgcca 4201 tcattttgga gaggattgaa tactgcaccc aggacccgga tgtaatcaac accgctttgc 4261 cgatagaata tggtccactt gtggaagagg aagagaaagt gcctgtgagg cccaaggacc 4321 ctgagggggt tcctcctctc ctggtctctc aacaggcaaa acgggaggag gagcgcagcc 4381 cagctgcccc accacctctg cctaccacct cctctggcaa ggctgcaaag aaacccacag 4441 ctgcagagat ctctgttcga gtccctagag ggccggccgt ggaaggggga cacgtgaata 4501 tggcattctc tcagtccaac cctccttcgg agttgcacaa ggtccacgga tccagaaaca 4561 agcccaccag cttgtggaac ccaacgtacg gctcctggtt tacagagaaa cccaccaaaa 4621 agaataatcc tatagcaaag aaggagccac acgacagggg taacctgggg ctggagggaa 4681 gctgtactgt cccacctaac gttgcaactg ggagacttcc gggggcctca ctgctcctag 4741 agccctcttc gctgactgcc aatatgaagg aggtacctct gttcaggcta cgtcacttcc 4801 cttgtgggaa tgtcaattac ggctaccagc aacagggctt gcccttagaa gccgctactg 4861 cccctggagc tggtcattac gaggatacca ttctgaaaag caagaatagc atgaaccagc 4921 ctgggccctt gccaactttc ttgtacaaag tggttggtaa gcctatccct aaccctctcc 4981 tcggtctcga ttctacgtag taatgagcta gcgctaaccg gtggcgcgtt aagtcgacaa 5041 tcaacctctg gatta