Construct: ORF ccsbBroad304_14540

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF019031.1_s304c1, BRDN0000459830
Derived from:
ccsbBroadEn_14540
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
ALK (238)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_14540
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 238 ALK ALK receptor tyrosine kinase NM_004304.5 100% 100%
2 human 238 ALK ALK receptor tyrosine kinase XR_001738688.2 54.8% 1_930del;3670_3671ins74;4104_4105ins1612
3 human 238 ALK ALK receptor tyrosine kinase XM_024452778.1 41.4% 41.4% 0_1ins2847
4 human 238 ALK ALK receptor tyrosine kinase NM_001353765.1 34% 34% 0_1ins3204
5 human 238 ALK ALK receptor tyrosine kinase XM_024452779.1 34% 34% 0_1ins3204
6 mouse 11682 Alk anaplastic lymphoma kinase NM_007439.2 83.6% 87.1% (many diffs)
7 mouse 11682 Alk anaplastic lymphoma kinase XR_001782029.1 53.9% (many diffs)
8 mouse 11682 Alk anaplastic lymphoma kinase XR_385265.3 43.2% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
4929
ORF length:
4860
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat gggagccatc gggctcctgt ggctcctgcc gctgctgctt tccacggcag
121ctgtgggctc cgggatgggg accggccagc gcgcgggctc cccagctgcg gggccgccgc
181tgcagccccg ggagccactc agctactcgc gcctgcagag gaagagtctg gcagttgact
241tcgtggtgcc ctcgctcttc cgtgtctacg cccgggacct actgctgcca ccatcctcct
301cggagctgaa ggctggcagg cccgaggccc gcggctcgct agctctggac tgcgccccgc
361tgctcaggtt gctggggccg gcgccggggg tctcctggac cgccggttca ccagccccgg
421cagaggcccg gacgctgtcc agggtgctga agggcggctc cgtgcgcaag ctccggcgtg
481ccaagcagtt ggtgctggag ctgggcgagg aggcgatctt ggagggttgc gtcgggcccc
541ccggggaggc ggctgtgggg ctgctccagt tcaatctcag cgagctgttc agttggtgga
601ttcgccaagg cgaagggcga ctgaggatcc gcctgatgcc cgagaagaag gcgtcggaag
661tgggcagaga gggaaggctg tccgcggcaa ttcgcgcctc ccagccccgc cttctcttcc
721agatcttcgg gactggtcat agctccttgg aatcaccaac aaacatgcct tctccttctc
781ctgattattt tacatggaat ctcacctgga taatgaaaga ctccttccct ttcctgtctc
841atcgcagccg atatggtctg gagtgcagct ttgacttccc ctgtgagctg gagtattccc
901ctccactgca tgacctcagg aaccagagct ggtcctggcg ccgcatcccc tccgaggagg
961cctcccagat ggacttgctg gatgggcctg gggcagagcg ttctaaggag atgcccagag
1021gctcctttct ccttctcaac acctcagctg actccaagca caccatcctg agtccgtgga
1081tgaggagcag cagtgagcac tgcacactgg ccgtctcggt gcacaggcac ctgcagccct
1141ctggaaggta cattgcccag ctgctgcccc acaacgaggc tgcaagagag atcctcctga
1201tgcccactcc agggaagcat ggttggacag tgctccaggg aagaatcggg cgtccagaca
1261acccatttcg agtggccctg gaatacatct ccagtggaaa ccgcagcttg tctgcagtgg
1321acttctttgc cctgaagaac tgcagtgaag gaacatcccc aggctccaag atggccctgc
1381agagctcctt cacttgttgg aatgggacag tcctccagct tgggcaggcc tgtgacttcc
1441accaggactg tgcccaggga gaagatgaga gccagatgtg ccggaaactg cctgtgggtt
1501tttactgcaa ctttgaagat ggcttctgtg gctggaccca aggcacactg tcaccccaca
1561ctcctcaatg gcaggtcagg accctaaagg atgcccggtt ccaggaccac caagaccatg
1621ctctattgct cagtaccact gatgtccccg cttctgaaag tgctacagtg accagtgcta
1681cgtttcctgc accgatcaag agctctccat gtgagctccg aatgtcctgg ctcattcgtg
1741gagtcttgag gggaaacgtg tccttggtgc tagtggagaa caaaaccggg aaggagcaag
1801gcaggatggt ctggcatgtc gccgcctatg aaggcttgag cctgtggcag tggatggtgt
1861tgcctctcct cgatgtgtct gacaggttct ggctgcagat ggtcgcatgg tggggacaag
1921gatccagagc catcgtggct tttgacaata tctccatcag cctggactgc tacctcacca
1981ttagcggaga ggacaagatc ctgcagaata cagcacccaa atcaagaaac ctgtttgaga
2041gaaacccaaa caaggagctg aaacccgggg aaaattcacc aagacagacc cccatctttg
2101accctacagt tcattggctg ttcaccacat gtggggccag cgggccccat ggccccaccc
2161aggcacagtg caacaacgcc taccagaact ccaacctgag cgtggaggtg gggagcgagg
2221gccccctgaa aggcatccag atctggaagg tgccagccac cgacacctac agcatctcgg
2281gctacggagc tgctggcggg aaaggcggga agaacaccat gatgcggtcc cacggcgtgt
2341ctgtgctggg catcttcaac ctggagaagg atgacatgct gtacatcctg gttgggcagc
2401agggagagga cgcctgcccc agtacaaacc agttaatcca gaaagtctgc attggagaga
2461acaatgtgat agaagaagaa atccgtgtga acagaagcgt gcatgagtgg gcaggaggcg
2521gaggaggagg gggtggagcc acctacgtat ttaagatgaa ggatggagtg ccggtgcccc
2581tgatcattgc agccggaggt ggtggcaggg cctacggggc caagacagac acgttccacc
2641cagagagact ggagaataac tcctcggttc tagggctaaa cggcaattcc ggagccgcag
2701gtggtggagg tggctggaat gataacactt ccttgctctg ggccggaaaa tctttgcagg
2761agggtgccac cggaggacat tcctgccccc aggccatgaa gaagtggggg tgggagacaa
2821gagggggttt cggagggggt ggaggggggt gctcctcagg tggaggaggc ggaggatata
2881taggcggcaa tgcagcctca aacaatgacc ccgaaatgga tggggaagat ggggtttcct
2941tcatcagtcc actgggcatc ctgtacaccc cagctttaaa agtgatggaa ggccacgggg
3001aagtgaatat taagcattat ctaaactgca gtcactgtga ggtagacgaa tgtcacatgg
3061accctgaaag ccacaaggtc atctgcttct gtgaccacgg gacggtgctg gctgaggatg
3121gcgtctcctg cattgtgtca cccaccccgg agccacacct gccactctcg ctgatcctct
3181ctgtggtgac ctctgccctc gtggccgccc tggtcctggc tttctccggc atcatgattg
3241tgtaccgccg gaagcaccag gagctgcaag ccatgcagat ggagctgcag agccctgagt
3301acaagctgag caagctccgc acctcgacca tcatgaccga ctacaacccc aactactgct
3361ttgctggcaa gacctcctcc atcagtgacc tgaaggaggt gccgcggaaa aacatcaccc
3421tcattcgggg tctgggccat ggcgcctttg gggaggtgta tgaaggccag gtgtccggaa
3481tgcccaacga cccaagcccc ctgcaagtgg ctgtgaagac gctgcctgaa gtgtgctctg
3541aacaggacga actggatttc ctcatggaag ccctgatcat cagcaaattc aaccaccaga
3601acattgttcg ctgcattggg gtgagcctgc aatccctgcc ccggttcatc ctgctggagc
3661tcatggcggg gggagacctc aagtccttcc tccgagagac ccgccctcgc ccgagccagc
3721cctcctccct ggccatgctg gaccttctgc acgtggctcg ggacattgcc tgtggctgtc
3781agtatttgga ggaaaaccac ttcatccacc gagacattgc tgccagaaac tgcctcttga
3841cctgtccagg ccctggaaga gtggccaaga ttggagactt cgggatggcc cgagacatct
3901acagggcgag ctactataga aagggaggct gtgccatgct gccagttaag tggatgcccc
3961cagaggcctt catggaagga atattcactt ctaaaacaga cacatggtcc tttggagtgc
4021tgctatggga aatcttttct cttggatata tgccataccc cagcaaaagc aaccaggaag
4081ttctggagtt tgtcaccagt ggaggccgga tggacccacc caagaactgc cctgggcctg
4141tataccggat aatgactcag tgctggcaac atcagcctga agacaggccc aactttgcca
4201tcattttgga gaggattgaa tactgcaccc aggacccgga tgtaatcaac accgctttgc
4261cgatagaata tggtccactt gtggaagagg aagagaaagt gcctgtgagg cccaaggacc
4321ctgagggggt tcctcctctc ctggtctctc aacaggcaaa acgggaggag gagcgcagcc
4381cagctgcccc accacctctg cctaccacct cctctggcaa ggctgcaaag aaacccacag
4441ctgcagagat ctctgttcga gtccctagag ggccggccgt ggaaggggga cacgtgaata
4501tggcattctc tcagtccaac cctccttcgg agttgcacaa ggtccacgga tccagaaaca
4561agcccaccag cttgtggaac ccaacgtacg gctcctggtt tacagagaaa cccaccaaaa
4621agaataatcc tatagcaaag aaggagccac acgacagggg taacctgggg ctggagggaa
4681gctgtactgt cccacctaac gttgcaactg ggagacttcc gggggcctca ctgctcctag
4741agccctcttc gctgactgcc aatatgaagg aggtacctct gttcaggcta cgtcacttcc
4801cttgtgggaa tgtcaattac ggctaccagc aacagggctt gcccttagaa gccgctactg
4861cccctggagc tggtcattac gaggatacca ttctgaaaag caagaatagc atgaaccagc
4921ctgggccctt gccaactttc ttgtacaaag tggttggtaa gcctatccct aaccctctcc
4981tcggtctcga ttctacgtag taatgagcta gcgctaaccg gtggcgcgtt aagtcgacaa
5041tcaacctctg gatta

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