Construct: ORF ccsbBroad304_14585

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF016663.2_s304c1, BRDN0000459782
Derived from:
ccsbBroadEn_14585
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
CKM (1158)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_14585
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 1158 CKM creatine kinase, M-type NM_001824.5 99.7% 99.7% 369T>C;673T>C;1119T>C
2 mouse 12715 Ckm creatine kinase, muscle NM_007710.2 90.3% 96.3% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1209
ORF length:
1143
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgcc attcggtaac acccacaaca agttcaagct gaattacaag cctgaggagg
121agtaccccga cctcagcaaa cataacaacc acatggccaa ggtactgacc cttgaactct
181acaagaagct gcgggacaag gagactccat ctggcttcac tgtagacgat gtcatccaga
241caggagtgga caacccaggt caccccttca tcatgaccgt gggctgcgtg gctggtgatg
301aggagtccta cgaagttttc aaggaactct ttgaccccat catctcggat cgccacgggg
361gctacaaacc cactgacaag cacaagactg acctcaacca tgaaaacctc aagggtggag
421acgacctgga ccccaactac gtgctcagca gccgcgtccg cactggccgc agcatcaagg
481gctacacgtt gcccccacac tgctcccgtg gcgagcgccg ggcggtggag aagctctctg
541tggaagctct caacagcctg acgggcgagt tcaaagggaa gtactaccct ctgaagagca
601tgacggagaa ggagcagcag cagctcatcg atgaccactt cctgttcgac aagcccgtgt
661ccccgctgct gctggcctca ggcatggccc gcgactggcc cgacgcccgt ggcatctggc
721acaatgacaa caagagcctc ctggtgtggg tgaacgagga ggatcacctc cgggtcatct
781ccatggagaa ggggggcaac atgaaggagg ttttccgccg cttctgcgta gggctgcaga
841agattgagga gatctttaag aaagctggcc accccttcat gtggaaccag cacctgggct
901acgtgctcac ctgcccatcc aacctgggca ctgggctgcg tggaggcgtg catgtgaagc
961tggcgcacct gagcaagcac cccaagttcg aggagatcct cacccgcctg cgtctgcaga
1021agaggggtac aggtggcgtg gacacagctg ccgtgggctc agtatttgac gtgtccaacg
1081ctgatcggct gggctcgtcc gaagtagaac aggtgcagct ggtggtggat ggtgtgaagc
1141tcatggtgga aatggagaag aagttggaga aaggccagtc catcgacgac atgatccccg
1201cccagaagta cccaactttc ttgtacaaag tggttggtaa gcctatccct aaccctctcc
1261tcggtctcga ttctacgtag taatgagcta gcgctaaccg gtggcgcgtt aagtcgacaa
1321tcaacctctg gatta

Download FASTA (ORF) (Full)