Construct: ORF ccsbBroad304_14597

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF019042.1_s304c1, BRDN0000459841
Derived from:
ccsbBroadEn_14597
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
CSF1R (1436)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_14597
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 1436 CSF1R colony stimulating factor 1... NM_001288705.2 100% 100%
2 human 1436 CSF1R colony stimulating factor 1... NM_001349736.1 100% 100%
3 human 1436 CSF1R colony stimulating factor 1... NM_005211.3 100% 100%
4 human 1436 CSF1R colony stimulating factor 1... NR_109969.1 70.1% 1_213del;2180_2181ins163;2967_3760del
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
2985
ORF length:
2916
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat gggcccagga gttctgctgc tcctgctggt ggccacagct tggcatggtc
121agggaatccc agtgatagag cccagtgtcc ctgagctggt cgtgaagcca ggagcaacgg
181tgaccttgcg atgtgtgggc aatggcagcg tggaatggga tggcccccca tcacctcact
241ggaccctgta ctctgatggc tccagcagca tcctcagcac caacaacgct accttccaaa
301acacggggac ctatcgctgc actgagcctg gagaccccct gggaggcagc gccgccatcc
361acctctatgt caaagaccct gcccggccct ggaacgtgct agcacaggag gtggtcgtgt
421tcgaggacca ggacgcacta ctgccctgtc tgctcacaga cccggtgctg gaagcaggcg
481tctcgctggt gcgtgtgcgt ggccggcccc tcatgcgcca caccaactac tccttctcgc
541cctggcatgg cttcaccatc cacagggcca agttcattca gagccaggac tatcaatgca
601gtgccctgat gggtggcagg aaggtgatgt ccatcagcat ccggctgaaa gtgcagaaag
661tcatcccagg gcccccagcc ttgacactgg tgcctgcaga gctggtgcgg attcgagggg
721aggctgccca gatcgtgtgc tcagccagca gcgttgatgt taactttgat gtcttcctcc
781aacacaacaa caccaagctc gcaatccctc aacaatctga ctttcataat aaccgttacc
841aaaaagtcct gaccctcaac ctcgatcaag tagatttcca acatgccggc aactactcct
901gcgtggccag caacgtgcag ggcaagcact ccacctccat gttcttccgg gtggtagaga
961gtgcctactt gaacttgagc tctgagcaga acctcatcca ggaggtgacc gtgggggagg
1021ggctcaacct caaagtcatg gtggaggcct acccaggcct gcaaggtttt aactggacct
1081acctgggacc cttttctgac caccagcctg agcccaagct tgctaatgct accaccaagg
1141acacatacag gcacaccttc accctctctc tgccccgcct gaagccctct gaggctggcc
1201gctactcctt cctggccaga aacccaggag gctggagagc tctgacgttt gagctcaccc
1261ttcgataccc cccagaggta agcgtcatat ggacattcat caacggctct ggcacccttt
1321tgtgtgctgc ctctgggtac ccccagccca acgtgacatg gctgcagtgc agtggccaca
1381ctgataggtg tgatgaggcc caagtgctgc aggtctggga tgacccatac cctgaggtcc
1441tgagccagga gcccttccac aaggtgacgg tgcagagcct gctgactgtt gagaccttag
1501agcacaacca aacctacgag tgcagggccc acaacagcgt ggggagtggc tcctgggcct
1561tcatacccat ctctgcagga gcccacacgc atcccccgga tgagttcctc ttcacaccag
1621tggtggtcgc ctgcatgtcc atcatggcct tgctgctgct gctgctcctg ctgctattgt
1681acaagtataa gcagaagccc aagtaccagg tccgctggaa gatcatcgag agctatgagg
1741gcaacagtta tactttcatc gaccccacgc agctgcctta caacgagaag tgggagttcc
1801cccggaacaa cctgcagttt ggtaagaccc tcggagctgg agcctttggg aaggtggtgg
1861aggccacggc ctttggtctg ggcaaggagg atgctgtcct gaaggtggct gtgaagatgc
1921tgaagtccac ggcccatgct gatgagaagg aggccctcat gtccgagctg aagatcatga
1981gccacctggg ccagcacgag aacatcgtca accttctggg agcctgtacc catggaggcc
2041ctgtactggt catcacggag tactgttgct atggcgacct gctcaacttt ctgcgaagga
2101aggctgaggc catgctggga cccagcctga gccccggcca ggaccccgag ggaggcgtcg
2161actataagaa catccacctc gagaagaaat atgtccgcag ggacagtggc ttctccagcc
2221agggtgtgga cacctatgtg gagatgaggc ctgtctccac ttcttcaaat gactccttct
2281ctgagcaaga cctggacaag gaggatggac ggcccctgga gctccgggac ctgcttcact
2341tctccagcca agtagcccag ggcatggcct tcctcgcttc caagaattgc atccaccggg
2401acgtggcagc gcgtaacgtg ctgttgacca atggtcatgt ggccaagatt ggggacttcg
2461ggctggctag ggacatcatg aatgactcca actacattgt caagggcaat gcccgcctgc
2521ctgtgaagtg gatggcccca gagagcatct ttgactgtgt ctacacggtt cagagcgacg
2581tctggtccta tggcatcctc ctctgggaga tcttctcact tgggctgaat ccctaccctg
2641gcatcctggt gaacagcaag ttctataaac tggtgaagga tggataccaa atggcccagc
2701ctgcatttgc cccaaagaat atatacagca tcatgcaggc ctgctgggcc ttggagccca
2761cccacagacc caccttccag cagatctgct ccttccttca ggagcaggcc caagaggaca
2821ggagagagcg ggactatacc aatctgccga gcagcagcag aagcggtggc agcggcagca
2881gcagcagtga gctggaggag gagagctcta gtgagcacct gacctgctgc gagcaagggg
2941atatcgccca gcccttgctg cagcccaaca actatcagtt ctgcttgcca actttcttgt
3001acaaagtggt tggtaagcct atccctaacc ctctcctcgg tctcgattct acgtagtaat
3061gagctagcgc taaccggtgg cgcgttaagt cgacaatcaa cctctggatt a

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