Construct: ORF ccsbBroad304_14631
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019002.1_s304c1, BRDN0000459803
- Derived from:
- ccsbBroadEn_14631
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ERBB2 (2064)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_14631
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2064 | ERBB2 | erb-b2 receptor tyrosine ki... | NM_004448.3 | 99.9% | 100% | 3764_3765delTG |
| 2 | human | 2064 | ERBB2 | erb-b2 receptor tyrosine ki... | NM_001289936.1 | 98.6% | 98.3% | (many diffs) |
| 3 | human | 2064 | ERBB2 | erb-b2 receptor tyrosine ki... | NM_001005862.2 | 97.5% | 97.6% | 0_1ins90;3674_3675delTG |
| 4 | human | 2064 | ERBB2 | erb-b2 receptor tyrosine ki... | XM_024450641.1 | 96.4% | 96.4% | 759_896del;3902_3903delTG |
| 5 | human | 2064 | ERBB2 | erb-b2 receptor tyrosine ki... | XM_024450642.1 | 95.1% | 94.8% | (many diffs) |
| 6 | human | 2064 | ERBB2 | erb-b2 receptor tyrosine ki... | XM_024450643.1 | 94.1% | 94.1% | 0_1ins90;669_806del;3812_3813delTG |
| 7 | human | 2064 | ERBB2 | erb-b2 receptor tyrosine ki... | NM_001289937.1 | 83.9% | 83.9% | 3161_3163delATAinsG;3165_3166ins600 |
| 8 | human | 2064 | ERBB2 | erb-b2 receptor tyrosine ki... | NR_110535.1 | 79.6% | 1_261del;484_546del;4088_4727del | |
| 9 | human | 2064 | ERBB2 | erb-b2 receptor tyrosine ki... | NM_001289938.1 | 48% | 48% | 0_1ins90;1809_1809delGins1865 |
| 10 | mouse | 13866 | Erbb2 | erb-b2 receptor tyrosine ki... | NM_001003817.1 | 85.6% | 87.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 3834
- ORF length:
- 3765
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggagctggcg gccttgtgcc gctgggggct cctcctcgcc ctcttgcccc 121 ccggagccgc gagcacccaa gtgtgcaccg gcacagacat gaagctgcgg ctccctgcca 181 gtcccgagac ccacctggac atgctccgcc acctctacca gggctgccag gtggtgcagg 241 gaaacctgga actcacctac ctgcccacca atgccagcct gtccttcctg caggatatcc 301 aggaggtgca gggctacgtg ctcatcgctc acaaccaagt gaggcaggtc ccactgcaga 361 ggctgcggat tgtgcgaggc acccagctct ttgaggacaa ctatgccctg gccgtgctag 421 acaatggaga cccgctgaac aataccaccc ctgtcacagg ggcctcccca ggaggcctgc 481 gggagctgca gcttcgaagc ctcacagaga tcttgaaagg aggggtcttg atccagcgga 541 acccccagct ctgctaccag gacacgattt tgtggaagga catcttccac aagaacaacc 601 agctggctct cacactgata gacaccaacc gctctcgggc ctgccacccc tgttctccga 661 tgtgtaaggg ctcccgctgc tggggagaga gttctgagga ttgtcagagc ctgacgcgca 721 ctgtctgtgc cggtggctgt gcccgctgca aggggccact gcccactgac tgctgccatg 781 agcagtgtgc tgccggctgc acgggcccca agcactctga ctgcctggcc tgcctccact 841 tcaaccacag tggcatctgt gagctgcact gcccagccct ggtcacctac aacacagaca 901 cgtttgagtc catgcccaat cccgagggcc ggtatacatt cggcgccagc tgtgtgactg 961 cctgtcccta caactacctt tctacggacg tgggatcctg caccctcgtc tgccccctgc 1021 acaaccaaga ggtgacagca gaggatggaa cacagcggtg tgagaagtgc agcaagccct 1081 gtgcccgagt gtgctatggt ctgggcatgg agcacttgcg agaggtgagg gcagttacca 1141 gtgccaatat ccaggagttt gctggctgca agaagatctt tgggagcctg gcatttctgc 1201 cggagagctt tgatggggac ccagcctcca acactgcccc gctccagcca gagcagctcc 1261 aagtgtttga gactctggaa gagatcacag gttacctata catctcagca tggccggaca 1321 gcctgcctga cctcagcgtc ttccagaacc tgcaagtaat ccggggacga attctgcaca 1381 atggcgccta ctcgctgacc ctgcaagggc tgggcatcag ctggctgggg ctgcgctcac 1441 tgagggaact gggcagtgga ctggccctca tccaccataa cacccacctc tgcttcgtgc 1501 acacggtgcc ctgggaccag ctctttcgga acccgcacca agctctgctc cacactgcca 1561 accggccaga ggacgagtgt gtgggcgagg gcctggcctg ccaccagctg tgcgcccgag 1621 ggcactgctg gggtccaggg cccacccagt gtgtcaactg cagccagttc cttcggggcc 1681 aggagtgcgt ggaggaatgc cgagtactgc aggggctccc cagggagtat gtgaatgcca 1741 ggcactgttt gccgtgccac cctgagtgtc agccccagaa tggctcagtg acctgttttg 1801 gaccggaggc tgaccagtgt gtggcctgtg cccactataa ggaccctccc ttctgcgtgg 1861 cccgctgccc cagcggtgtg aaacctgacc tctcctacat gcccatctgg aagtttccag 1921 atgaggaggg cgcatgccag ccttgcccca tcaactgcac ccactcctgt gtggacctgg 1981 atgacaaggg ctgccccgcc gagcagagag ccagccctct gacgtccatc atctctgcgg 2041 tggttggcat tctgctggtc gtggtcttgg gggtggtctt tgggatcctc atcaagcgac 2101 ggcagcagaa gatccggaag tacacgatgc ggagactgct gcaggaaacg gagctggtgg 2161 agccgctgac acctagcgga gcgatgccca accaggcgca gatgcggatc ctgaaagaga 2221 cggagctgag gaaggtgaag gtgcttggat ctggcgcttt tggcacagtc tacaagggca 2281 tctggatccc tgatggggag aatgtgaaaa ttccagtggc catcaaagtg ttgagggaaa 2341 acacatcccc caaagccaac aaagaaatct tagacgaagc atacgtgatg gctggtgtgg 2401 gctccccata tgtctcccgc cttctgggca tctgcctgac atccacggtg cagctggtga 2461 cacagcttat gccctatggc tgcctcttag accatgtccg ggaaaaccgc ggacgcctgg 2521 gctcccagga cctgctgaac tggtgtatgc agattgccaa ggggatgagc tacctggagg 2581 atgtgcggct cgtacacagg gacttggccg ctcggaacgt gctggtcaag agtcccaacc 2641 atgtcaaaat tacagacttc gggctggctc ggctgctgga cattgacgag acagagtacc 2701 atgcagatgg gggcaaggtg cccatcaagt ggatggcgct ggagtccatt ctccgccggc 2761 ggttcaccca ccagagtgat gtgtggagtt atggtgtgac tgtgtgggag ctgatgactt 2821 ttggggccaa accttacgat gggatcccag cccgggagat ccctgacctg ctggaaaagg 2881 gggagcggct gccccagccc cccatctgca ccattgatgt ctacatgatc atggtcaaat 2941 gttggatgat tgactctgaa tgtcggccaa gattccggga gttggtgtct gaattctccc 3001 gcatggccag ggacccccag cgctttgtgg tcatccagaa tgaggacttg ggcccagcca 3061 gtcccttgga cagcaccttc taccgctcac tgctggagga cgatgacatg ggggacctgg 3121 tggatgctga ggagtatctg gtaccccagc agggcttctt ctgtccagac cctgccccgg 3181 gcgctggggg catggtccac cacaggcacc gcagctcatc taccaggagt ggcggtgggg 3241 acctgacact agggctggag ccctctgaag aggaggcccc caggtctcca ctggcacccT 3301 CCGAAGGGGC TGGCTCCGAT GTATTTGATG GTGACCTGGG AATGGGGGCA GCCAAGGGGC 3361 TGCAAAGCCT CCCCACACAT GACCCCAGCC CTCTACAGCG GTACAGTGAG GACCCCACAG 3421 TACCCCTGCC CTCTGAGACT GATGGCTACG TTGCCCCCCT GACCTGCAGC CCCCAGCCTG 3481 AATATGTGAA CCAGCCAGAT GTTCGGCCCC AGCCCCCTTC GCCCCGAGAG GGCCCTCTGC 3541 CTGCTGCCCG ACCTGCTGGT GCCACTCTGG AAAGGCCCAA GACTCTCTCC CCAGGGAAGA 3601 ATGGGGTCGT CAAAGACGTT TTTGCCTTTG GGGGTGCCGT GGAGAACCCC GAGTACTTGA 3661 CACCCCAGGG AGGAGCTGCC CCTCAGCCCC ACCCTCCTCC TGCCTTCAGC CCAGCCTTCG 3721 ACAACCTCTA TTACTGGGAC CAGGACCCAC CAGAGCGGGG GGCTCCACCC AGCACCTTCA 3781 AAGGGACACC TACGGCAGAG AACCCAGAGT ACCTGGGTCT GGACGTGCCA GTGCCAACTT 3841 TCTTGTACaa agtggttggt aagcctatcc ctaaccctct cctcggtctc gattctacgt 3901 agtaatgagc tagcgctaac cggtggcgcg ttaagtcgac aatcaacctc tggatta