Construct: ORF ccsbBroad304_14742
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018850.1_s304c1, BRDN0000459334
- Derived from:
- ccsbBroadEn_14742
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- PFKFB1 (5207)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_14742
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5207 | PFKFB1 | 6-phosphofructo-2-kinase/fr... | NM_001271805.1 | 67.1% | 48.6% | (many diffs) |
| 2 | human | 5207 | PFKFB1 | 6-phosphofructo-2-kinase/fr... | NM_002625.4 | 61.8% | 42.5% | (many diffs) |
| 3 | human | 5207 | PFKFB1 | 6-phosphofructo-2-kinase/fr... | XM_017029577.1 | 57.6% | 42.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1002
- ORF length:
- 933
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gaagctctgc gtgactgtcc tgtctctcct catgctagta gctgccttct 121 gctctccagc gctctcagca ccaagtaagt ctacttttgc agctgctatt tcgagtcaag 181 gtgtaggcag agtccttttt tctagtcatg gctggcaaac agtgggatct ggggatggga 241 caaaaggcag cttgccaact ttcttgtaca aagttggcat tatcagaaag cattgcttat 301 cacttcgctg caacgaacag gtcaccatca gtcaaaataa aatcattatt gccatccagc 361 tgattcccga cataattgca gaaaacatca ggcaagtgaa acttggcagc cctgattata 421 tagactgtga ccgggaaaag gttctggaag actttctaaa gagaattgag tgctatgagg 481 tcaactacca acccttggat gaggaactgg acagccacct gtcctacatc aagatcttcg 541 acgtgggcac acgctacatg gtgaaccgag tgcaggatca catccagagc cgcacagtct 601 actacctcat gaatatccat gtcacacctc gctccatcta cctttgccga catggcgaga 661 gtgaactcaa catcagaggc cgcatcggag gtgactctgg cctctcagtt cgcggcaagc 721 agtatgccta tgccctggcc aacttcattc agtcccaggg catcagctcc ctgaaggtgt 781 ggaccagtca catgaagagg accatccaga cagctgaggc cctgggtgtc ccctatgagc 841 agtggaaggc cctgaatgag attgatgcgg gtgtctgtga ggagatgacc tatgaagaaa 901 tccaggaaca ttaccctgaa gaatttgcac tgcgagacca agataaatat cgctaccgct 961 atcccaaggg agagtcctat tatgatttta ttttgactcg atagtcgacc tattcgttgc 1021 aagcaaattc gatgagcaat gcttttttat aatgccaact ttgtacaaaa aagttggcac 1081 catgaagctc tgcgtgactg tcctgtctct cctcatgcta gtagctgcct tctgctctcc 1141 agcgctctca gcaccaagta agtctacttt tgcagctgct atttcgagtc aaggtgtagg 1201 cagagtcctt ttttctagtc atggctggca aacagtggga tctggggatg ggacaaaagg 1261 cagcttgcca actttcttgt acaaagtggt tggtaagcct atccctaacc ctctcctcgg 1321 tctcgattct acgtagtaat gagctagcgc taaccggtgg cgcgttaagt cgacaatcaa 1381 cctctggatt a