Construct: ORF ccsbBroad304_14771
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007548.2_s304c1, BRDN0000459591
- Derived from:
- ccsbBroadEn_14771
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EXOSC10 (5394)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_14771
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5394 | EXOSC10 | exosome component 10 | NM_001001998.3 | 99.9% | 100% | 1389G>C;2169C>T |
| 2 | human | 5394 | EXOSC10 | exosome component 10 | XM_005263475.2 | 99.3% | 99.4% | 1389G>C;2169C>T;2240_2254del |
| 3 | human | 5394 | EXOSC10 | exosome component 10 | NM_002685.4 | 97% | 97.1% | 1389G>C;2082_2083ins75;2094C>T |
| 4 | human | 5394 | EXOSC10 | exosome component 10 | XR_002956819.1 | 72.6% | (many diffs) | |
| 5 | human | 5394 | EXOSC10 | exosome component 10 | XR_002956818.1 | 72.3% | (many diffs) | |
| 6 | human | 5394 | EXOSC10 | exosome component 10 | XR_002956822.1 | 69.9% | (many diffs) | |
| 7 | human | 5394 | EXOSC10 | exosome component 10 | XR_002956821.1 | 69.6% | (many diffs) | |
| 8 | human | 5394 | EXOSC10 | exosome component 10 | XR_002956820.1 | 67.4% | (many diffs) | |
| 9 | human | 5394 | EXOSC10 | exosome component 10 | XM_017001495.2 | 52.9% | 51.7% | (many diffs) |
| 10 | human | 5394 | EXOSC10 | exosome component 10 | XM_017001497.2 | 51.1% | 51.1% | 0_1ins1296;93G>C;873C>T |
| 11 | mouse | 50912 | Exosc10 | exosome component 10 | NM_016699.2 | 83% | 87% | (many diffs) |
| 12 | mouse | 50912 | Exosc10 | exosome component 10 | XM_017320311.1 | 75.2% | 78.6% | (many diffs) |
| 13 | mouse | 50912 | Exosc10 | exosome component 10 | XM_017320312.1 | 68.2% | 72.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2721
- ORF length:
- 2655
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc gccacccagt acccgggagc ccagggtcct gtcggcgacc agcgcaacca 121 aatccgacgg agagatggtg ctgccaggct tcccggacgc cgacagcttt gtgaagtttg 181 ctcttgggtc cgtggtggca gtcaccaagg catctggggg cctaccacag tttggcgatg 241 agtatgattt ttaccgaagt tttcctggct tccaagcatt ttgcgaaaca cagggagaca 301 ggttgcttca gtgcatgagc agagtaatgc agtaccatgg gtgtcgcagc aacattaagg 361 atcgaagtaa agtgactgag ctggaagaca agtttgattt actagttgat gccaatgatg 421 taattctgga gagagtgggt attttactgg atgaagcctc aggtgtaaac aagaatcaac 481 agcctgtcct ccctgccggc ttgcaggtcc ccaaaacggt agtgtccagc tggaaccgta 541 aggcagcaga atatggcaaa aaagcaaaat ctgaaacttt ccggctgctt catgcaaaaa 601 atatcatccg acctcagctc aagtttcgag agaagattga caattccaac acaccatttc 661 ttcctaaaat cttcatcaaa cccaatgctc agaaacctct ccctcaagct ctctctaagg 721 aaaggcggga acgcccacag gatcgtcctg aggacttgga cgtcccccct gcactggctg 781 atttcatcca tcagcagaga acccagcagg ttgagcaaga catgtttgca catccttatc 841 aatatgaact aaatcacttt accccagcag atgcagtgct tcaaaagcca caaccccagt 901 tatacagacc tatagaagag acaccatgcc atttcatatc ctccctggat gaactcgtgg 961 aactcaacga aaagctcttg aattgtcagg aatttgcagt tgacttggag caccactctt 1021 acaggagctt cctgggactg acctgcctga tgcaaatttc tactcggacg gaagacttca 1081 tcattgacac cctcgagctt cgaagtgaca tgtacattct caatgagagc ctcacagacc 1141 cagccatcgt taaggtcttt catggtgctg attcagacat agaatggcta cagaaagact 1201 ttgggttgta tgtagtaaac atgtttgata ctcatcaggc agcacgcctt cttaacctgg 1261 gcaggcactc actcgatcat ctcctgaaac tctactgcaa cgtggactca aacaagcaat 1321 atcagctggc tgattggaga atacgccctc tgcccgagga gatgctcagc tacgcccggg 1381 atgacaccca ttacctgcta tatatctatg acaaaatgag gctggagatg tgggagcgcg 1441 gcaacgggca gcccgtgcag ctgcaggtgg tgtggcaacg gagcagggac atctgcctca 1501 agaaattcat caaacctatc ttcacggatg agtcctacct tgaactctat aggaagcaga 1561 agaagcacct taacacacag cagttgacag cctttcagct gctgtttgcc tggagggata 1621 aaacagctcg cagggaagat gaaagttacg gatatgtact gccaaaccac atgatgctga 1681 aaatagctga agaactgcct aaggaacctc agggcatcat agcttgctgc aacccagtac 1741 cgccccttgt gcggcagcag atcaacgaaa tgcacctttt aatccagcag gcccgagaga 1801 tgcccctgct caagtctgaa gttgcagccg gagtgaagaa gagcggaccg ctgcccagtg 1861 ctgagagatt ggagaatgtt ctctttggac ctcacgactg ctcccatgcc cctccggatg 1921 gctatccaat catcccaacc agtggatctg tgccagttca gaagcaggcg agcctcttcc 1981 ctgatgaaaa agaagataac ttgctgggta ccacatgcct gattgccaca gctgtcatca 2041 cgttatttaa tgaacctagt gctgaagaca gtaaaaaggg tccattgaca gttgcacaga 2101 aaaaagccca gaacatcatg gagtcctttg aaaatccatt taggatgttt ctgccctcac 2161 tgggacaccg tgctcccgtc tctcaggcag cgaagttcga tccatcaacc aaaatctatg 2221 aaatcagcaa ccgttggaag ctggcccagg tacaagtaca aaaagactct aaagaagctg 2281 tcaagaagaa ggcagctgag caaacagctg cccgggaaca ggcaaaggag gcgtgcaaag 2341 ctgcagcaga acaggccatc tccgtccgac agcaggtcgt gctagaaaat gctgcaaaga 2401 agagagagcg agcaacaagc gacccaagga ccacagaaca gaaacaagag aagaaacgac 2461 tcaaaatttc caagaagcca aaggacccag agccaccaga aaaagagttt acgccttacg 2521 actacagcca gtcagacttc aaggcttttg ctggaaacag caaatccaaa gtttcttctc 2581 agtttgatcc aaataaacag accccgtctg gcaagaaatg cattgcagcc aaaaaaatta 2641 aacagtcggt gggaaacaaa agcatgtcct ttccaactgg aaagtcagac agaggcttca 2701 ggtacaactg gccacagaga tacccaactt tcttgtacaa agtggttggt aagcctatcc 2761 ctaaccctct cctcggtctc gattctacgt agtaatgagc tagcgctaac cggtggcgcg 2821 ttaagtcgac aatcaacctc tggatta