Construct: ORF ccsbBroad304_14815

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF013091.2_s304c1, BRDN0000459506
Derived from:
ccsbBroadEn_14815
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PRPS1 (5631)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_14815
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5631 PRPS1 phosphoribosyl pyrophosphat... NM_002764.4 100% 100%
2 human 221823 PRPS1L1 phosphoribosyl pyrophosphat... NM_175886.3 92.2% 94% (many diffs)
3 human 5631 PRPS1 phosphoribosyl pyrophosphat... NM_001204402.1 35.8% 35.8% 0_1ins612
4 mouse 19139 Prps1 phosphoribosyl pyrophosphat... NM_021463.4 92.4% 100% (many diffs)
5 mouse 328099 Prps1l3 phosphoribosyl pyrophosphat... NM_001037746.3 92.4% 99.6% (many diffs)
6 mouse 75456 Prps1l1 phosphoribosyl pyrophosphat... NM_029294.2 87.7% 95.2% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1020
ORF length:
954
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgcc gaatatcaaa atcttcagcg gcagctccca ccaggactta tctcagaaaa
121ttgctgaccg cctgggcctg gagctaggca aggtggtgac taagaaattc agcaaccagg
181agacctgtgt ggaaattggt gaaagtgtac gtggagagga tgtctacatt gttcagagtg
241gttgtggcga aatcaatgac aatttaatgg agcttttgat catgattaat gcctgcaaga
301ttgcttcagc cagccgggtt actgcagtca tcccatgctt cccttatgcc cggcaggata
361agaaagataa gagccgggcg ccaatctcag ccaagcttgt tgcaaatatg ctatctgtag
421caggtgcaga tcatattatc accatggacc tacatgcttc tcaaattcag ggcttttttg
481atatcccagt agacaatttg tatgcagagc cggctgtcct aaagtggata agggagaata
541tctctgagtg gaggaactgc actattgtct cacctgatgc tggtggagct aagagagtga
601cctccattgc agacaggctg aatgtggact ttgccttgat tcacaaagaa cggaagaagg
661ccaatgaagt ggaccgcatg gtgcttgtgg gagatgtgaa ggatcgggtg gccatccttg
721tggatgacat ggctgacact tgtggcacaa tctgccatgc agctgacaaa cttctctcag
781ctggcgccac cagagtttat gccatcttga ctcatggaat cttctccggt cctgctattt
841ctcgcatcaa caacgcatgc tttgaggcag tagtagtcac caataccata cctcaggagg
901acaagatgaa gcattgctcc aaaatacagg tgattgacat ctctatgatc cttgcagaag
961ccatcaggag aactcacaat ggagaatccg tttcttacct attcagccat gtccctttat
1021acccaacttt cttgtacaaa gtggttggta agcctatccc taaccctctc ctcggtctcg
1081attctacgta gtaatgagct agcgctaacc ggtggcgcgt taagtcgaca atcaacctct
1141ggatta

Download FASTA (ORF) (Full)