Construct: ORF ccsbBroad304_14870
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019022.1_s304c1, BRDN0000459821
- Derived from:
- ccsbBroadEn_14870
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TYK2 (7297)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_14870
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7297 | TYK2 | tyrosine kinase 2 | NM_003331.5 | 99.9% | 99.9% | 3046G>T |
| 2 | human | 7297 | TYK2 | tyrosine kinase 2 | XM_011528245.1 | 99.9% | 99.9% | 3046G>T |
| 3 | human | 7297 | TYK2 | tyrosine kinase 2 | XM_011528246.3 | 91.6% | 91.5% | 0_1ins297;2749G>T |
| 4 | human | 7297 | TYK2 | tyrosine kinase 2 | XM_011528247.1 | 91.6% | 91.5% | 0_1ins297;2749G>T |
| 5 | human | 7297 | TYK2 | tyrosine kinase 2 | XR_001753750.1 | 85.4% | (many diffs) | |
| 6 | human | 7297 | TYK2 | tyrosine kinase 2 | XR_001753751.1 | 75% | (many diffs) | |
| 7 | human | 7297 | TYK2 | tyrosine kinase 2 | XR_002958353.1 | 62.9% | (many diffs) | |
| 8 | human | 7297 | TYK2 | tyrosine kinase 2 | XM_011528249.2 | 62.4% | 61.7% | (many diffs) |
| 9 | human | 7297 | TYK2 | tyrosine kinase 2 | XR_001753752.1 | 61.4% | 1_157del;2205_2316del;2623_2624ins1207 | |
| 10 | human | 7297 | TYK2 | tyrosine kinase 2 | XM_011528251.1 | 51% | 45.7% | 0_1ins1624;146_147ins119;1303G>T |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 3630
- ORF length:
- 3561
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gcctctgcgc cactggggga tggccagggg cagtaagccc gttggggatg 121 gagcccagcc catggctgcc atgggaggcc tgaaggtgct tctgcactgg gctggtccag 181 gcggcgggga gccctgggtc actttcagtg agtcatcgct gacagctgag gaagtctgca 241 tccacattgc acataaagtt ggtatcactc ctccttgctt caatctcttt gccctcttcg 301 atgctcaggc ccaagtctgg ttgcccccaa accacatcct agagatcccc agagatgcaa 361 gcctgatgct atatttccgc ataaggtttt atttccggaa ctggcatggc atgaatcctc 421 gggaaccggc tgtgtaccgt tgtgggcccc caggaaccga ggcatcctca gatcagacag 481 cacaggggat gcaactcctg gacccagcct catttgagta cctctttgag cagggcaagc 541 atgagtttgt gaatgacgtg gcatcactgt gggagctgtc gaccgaggag gagatccacc 601 actttaagaa tgagagcctg ggcatggcct ttctgcacct ctgtcacctc gctctccgcc 661 atggcatccc cctggaggag gtggccaaga agaccagctt caaggactgc atcccgcgct 721 ccttccgccg gcatatccgg cagcacagcg ccctgacccg gctgcgcctt cggaacgtct 781 tccgcaggtt cctgcgggac ttccagccgg gccgactctc ccagcagatg gtcatggtca 841 aatacctagc cacactcgag cggctggcac cccgcttcgg cacagagcgt gtgcccgtgt 901 gccacctgag gctgctggcc caggccgagg gggagccctg ctacatccgg gacagtgggg 961 tggcccctac agaccctggc cctgagtctg ctgctgggcc cccaacccac gaggtgctgg 1021 tgacaggcac tggtggcatc cagtggtggc cagtagagga ggaggtgaac aaggaggagg 1081 gttctagtgg cagcagtggc aggaaccccc aagccagcct gtttgggaag aaggccaagg 1141 ctcacaaggc agtcggccag ccggcagaca ggccgcggga gccactgtgg gcctacttct 1201 gtgacttccg ggacatcacc cacgtggtgc tgaaagagca ctgtgtcagc atccaccggc 1261 aggacaacaa gtgcctggag ctgagcttgc cttcccgggc tgcggcgctg tccttcgtgt 1321 cgctggtgga cggctatttc cgcctgacgg ccgactccag ccactacctg tgccacgagg 1381 tggctccccc acggctggtg atgagcatcc gggatgggat ccacggaccc ctgctggagc 1441 catttgtgca ggccaagctg cggcccgagg acggcctgta cctcattcac tggagcacca 1501 gccaccccta ccgcctgatc ctcacagtgg cccagcgtag ccaggcacca gacggcatgc 1561 agagcttgcg gctccgaaag ttccccattg agcagcagga cggggccttc gtgctggagg 1621 gctggggccg gtccttcccc agcgttcggg aacttggggc tgccttgcag ggctgcttgc 1681 tgagggccgg ggatgactgc ttctctctgc gtcgctgttg cctgccccaa ccaggagaaa 1741 cctccaatct catcatcatg cggggggctc gggccagccc caggacactc aacctcagcc 1801 agctcagctt ccaccgggtt gaccagaagg agatcaccca gctgtcccac ttgggccagg 1861 gcacaaggac caacgtgtat gagggccgcc tgcgagtgga gggcagcggg gaccctgagg 1921 agggcaagat ggatgacgag gaccccctcg tgcctggcag ggaccgtggg caggagctac 1981 gagtggtgct caaagtgctg gaccctagtc accatgacat cgccctggcc ttctacgaga 2041 cagccagcct catgagccag gtctcccaca cgcacctggc cttcgtgcat ggcgtctgtg 2101 tgcgcggccc tgaaaatatc atggtgacag agtacgtgga gcacggaccc ctggatgtgt 2161 ggctgcggag ggagcggggc catgtgccca tggcttggaa gatggtggtg gcccagcagc 2221 tggccagcgc cctcagctac ctggagaaca agaacctggt tcatggtaat gtgtgtggcc 2281 ggaacatcct gctggcccgg ctggggttgg cagagggcac cagccccttc atcaagctga 2341 gtgatcctgg cgtgggcctg ggcgccctct ccagggagga gcgggtggag aggatcccct 2401 ggctggcccc cgaatgccta ccaggtgggg ccaacagcct aagcaccgcc atggacaagt 2461 gggggtttgg cgccaccctc ctggagatct gctttgacgg agaggcccct ctgcagagcc 2521 gcagtccctc cgagaaggag catttctacc agaggcagca ccggctgccc gagccctcct 2581 gcccacagct ggccacactc accagccagt gtctgaccta tgagccaacc cagaggccat 2641 cattccgcac catcctgcgt gacctcaccc ggctgcagcc ccacaatctt gctgacgtct 2701 tgactgtgaa cccggactca ccggcgtcgg accctacggt tttccacaag cgctatttga 2761 aaaagatccg agatctgggc gagggtcact tcggcaaggt cagcttgtac tgctacgatc 2821 cgaccaacga cggcactggc gagatggtgg cggtgaaagc cctcaaggca gactgcggcc 2881 cccagcaccg ctcgggctgg aagcaggaga ttgacattct gcgcacgctc taccacgagc 2941 acatcatcaa gtacaagggc tgctgcgagg accaaggcga gaagtcgctg cagctggtca 3001 tggagtacgt gcccctgggc agcctccgag actacctgcc ccggcacagc atcgggctgg 3061 cccagctgct gctcttcgcc cagcagatct gcgagggcat ggcctatctg cactcgcagc 3121 actacatcca ccgagaccta gccgcgcgca acgtgctgct ggacaacgac aggctggtca 3181 agatcgggga ctttggccta gccaaggccg tgcccgaagg ccacgagtac taccgcgtgc 3241 gcgaggatgg ggacagcccc gtgttctggt atgccccaga gtgcctgaag gagtataagt 3301 tctactatgc gtcagatgtc tggtccttcg gggtgaccct gtatgagctg ctgacgcact 3361 gtgactccag ccagagcccc cccacgaaat tccttgagct cataggcatt gctcagggtc 3421 agatgacagt tctgagactc actgagttgc tggaacgagg ggagaggctg ccacggcccg 3481 acaaatgtcc ctgtgaggtc tatcatctca tgaagaactg ctgggagaca gaggcgtcct 3541 ttcgcccaac cttcgagaac ctcataccca ttctgaagac agtccatgag aagtaccaag 3601 gccaggcccc ttcagtgttc agcgtgtgct tgccaacttt cttgtacaaa gtggttggta 3661 agcctatccc taaccctctc ctcggtctcg attctacgta gtaatgagct agcgctaacc 3721 ggtggcgcgt taagtcgaca atcaacctct ggatta