Construct: ORF ccsbBroad304_14870

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF019022.1_s304c1, BRDN0000459821
Derived from:
ccsbBroadEn_14870
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
TYK2 (7297)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_14870
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 7297 TYK2 tyrosine kinase 2 NM_003331.5 99.9% 99.9% 3046G>T
2 human 7297 TYK2 tyrosine kinase 2 XM_011528245.1 99.9% 99.9% 3046G>T
3 human 7297 TYK2 tyrosine kinase 2 XM_011528246.3 91.6% 91.5% 0_1ins297;2749G>T
4 human 7297 TYK2 tyrosine kinase 2 XM_011528247.1 91.6% 91.5% 0_1ins297;2749G>T
5 human 7297 TYK2 tyrosine kinase 2 XR_001753750.1 85.4% (many diffs)
6 human 7297 TYK2 tyrosine kinase 2 XR_001753751.1 75% (many diffs)
7 human 7297 TYK2 tyrosine kinase 2 XR_002958353.1 62.9% (many diffs)
8 human 7297 TYK2 tyrosine kinase 2 XM_011528249.2 62.4% 61.7% (many diffs)
9 human 7297 TYK2 tyrosine kinase 2 XR_001753752.1 61.4% 1_157del;2205_2316del;2623_2624ins1207
10 human 7297 TYK2 tyrosine kinase 2 XM_011528251.1 51% 45.7% 0_1ins1624;146_147ins119;1303G>T
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
3630
ORF length:
3561
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat gcctctgcgc cactggggga tggccagggg cagtaagccc gttggggatg
121gagcccagcc catggctgcc atgggaggcc tgaaggtgct tctgcactgg gctggtccag
181gcggcgggga gccctgggtc actttcagtg agtcatcgct gacagctgag gaagtctgca
241tccacattgc acataaagtt ggtatcactc ctccttgctt caatctcttt gccctcttcg
301atgctcaggc ccaagtctgg ttgcccccaa accacatcct agagatcccc agagatgcaa
361gcctgatgct atatttccgc ataaggtttt atttccggaa ctggcatggc atgaatcctc
421gggaaccggc tgtgtaccgt tgtgggcccc caggaaccga ggcatcctca gatcagacag
481cacaggggat gcaactcctg gacccagcct catttgagta cctctttgag cagggcaagc
541atgagtttgt gaatgacgtg gcatcactgt gggagctgtc gaccgaggag gagatccacc
601actttaagaa tgagagcctg ggcatggcct ttctgcacct ctgtcacctc gctctccgcc
661atggcatccc cctggaggag gtggccaaga agaccagctt caaggactgc atcccgcgct
721ccttccgccg gcatatccgg cagcacagcg ccctgacccg gctgcgcctt cggaacgtct
781tccgcaggtt cctgcgggac ttccagccgg gccgactctc ccagcagatg gtcatggtca
841aatacctagc cacactcgag cggctggcac cccgcttcgg cacagagcgt gtgcccgtgt
901gccacctgag gctgctggcc caggccgagg gggagccctg ctacatccgg gacagtgggg
961tggcccctac agaccctggc cctgagtctg ctgctgggcc cccaacccac gaggtgctgg
1021tgacaggcac tggtggcatc cagtggtggc cagtagagga ggaggtgaac aaggaggagg
1081gttctagtgg cagcagtggc aggaaccccc aagccagcct gtttgggaag aaggccaagg
1141ctcacaaggc agtcggccag ccggcagaca ggccgcggga gccactgtgg gcctacttct
1201gtgacttccg ggacatcacc cacgtggtgc tgaaagagca ctgtgtcagc atccaccggc
1261aggacaacaa gtgcctggag ctgagcttgc cttcccgggc tgcggcgctg tccttcgtgt
1321cgctggtgga cggctatttc cgcctgacgg ccgactccag ccactacctg tgccacgagg
1381tggctccccc acggctggtg atgagcatcc gggatgggat ccacggaccc ctgctggagc
1441catttgtgca ggccaagctg cggcccgagg acggcctgta cctcattcac tggagcacca
1501gccaccccta ccgcctgatc ctcacagtgg cccagcgtag ccaggcacca gacggcatgc
1561agagcttgcg gctccgaaag ttccccattg agcagcagga cggggccttc gtgctggagg
1621gctggggccg gtccttcccc agcgttcggg aacttggggc tgccttgcag ggctgcttgc
1681tgagggccgg ggatgactgc ttctctctgc gtcgctgttg cctgccccaa ccaggagaaa
1741cctccaatct catcatcatg cggggggctc gggccagccc caggacactc aacctcagcc
1801agctcagctt ccaccgggtt gaccagaagg agatcaccca gctgtcccac ttgggccagg
1861gcacaaggac caacgtgtat gagggccgcc tgcgagtgga gggcagcggg gaccctgagg
1921agggcaagat ggatgacgag gaccccctcg tgcctggcag ggaccgtggg caggagctac
1981gagtggtgct caaagtgctg gaccctagtc accatgacat cgccctggcc ttctacgaga
2041cagccagcct catgagccag gtctcccaca cgcacctggc cttcgtgcat ggcgtctgtg
2101tgcgcggccc tgaaaatatc atggtgacag agtacgtgga gcacggaccc ctggatgtgt
2161ggctgcggag ggagcggggc catgtgccca tggcttggaa gatggtggtg gcccagcagc
2221tggccagcgc cctcagctac ctggagaaca agaacctggt tcatggtaat gtgtgtggcc
2281ggaacatcct gctggcccgg ctggggttgg cagagggcac cagccccttc atcaagctga
2341gtgatcctgg cgtgggcctg ggcgccctct ccagggagga gcgggtggag aggatcccct
2401ggctggcccc cgaatgccta ccaggtgggg ccaacagcct aagcaccgcc atggacaagt
2461gggggtttgg cgccaccctc ctggagatct gctttgacgg agaggcccct ctgcagagcc
2521gcagtccctc cgagaaggag catttctacc agaggcagca ccggctgccc gagccctcct
2581gcccacagct ggccacactc accagccagt gtctgaccta tgagccaacc cagaggccat
2641cattccgcac catcctgcgt gacctcaccc ggctgcagcc ccacaatctt gctgacgtct
2701tgactgtgaa cccggactca ccggcgtcgg accctacggt tttccacaag cgctatttga
2761aaaagatccg agatctgggc gagggtcact tcggcaaggt cagcttgtac tgctacgatc
2821cgaccaacga cggcactggc gagatggtgg cggtgaaagc cctcaaggca gactgcggcc
2881cccagcaccg ctcgggctgg aagcaggaga ttgacattct gcgcacgctc taccacgagc
2941acatcatcaa gtacaagggc tgctgcgagg accaaggcga gaagtcgctg cagctggtca
3001tggagtacgt gcccctgggc agcctccgag actacctgcc ccggcacagc atcgggctgg
3061cccagctgct gctcttcgcc cagcagatct gcgagggcat ggcctatctg cactcgcagc
3121actacatcca ccgagaccta gccgcgcgca acgtgctgct ggacaacgac aggctggtca
3181agatcgggga ctttggccta gccaaggccg tgcccgaagg ccacgagtac taccgcgtgc
3241gcgaggatgg ggacagcccc gtgttctggt atgccccaga gtgcctgaag gagtataagt
3301tctactatgc gtcagatgtc tggtccttcg gggtgaccct gtatgagctg ctgacgcact
3361gtgactccag ccagagcccc cccacgaaat tccttgagct cataggcatt gctcagggtc
3421agatgacagt tctgagactc actgagttgc tggaacgagg ggagaggctg ccacggcccg
3481acaaatgtcc ctgtgaggtc tatcatctca tgaagaactg ctgggagaca gaggcgtcct
3541ttcgcccaac cttcgagaac ctcataccca ttctgaagac agtccatgag aagtaccaag
3601gccaggcccc ttcagtgttc agcgtgtgct tgccaacttt cttgtacaaa gtggttggta
3661agcctatccc taaccctctc ctcggtctcg attctacgta gtaatgagct agcgctaacc
3721ggtggcgcgt taagtcgaca atcaacctct ggatta

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