Construct: ORF ccsbBroad304_14896
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017781.1_s304c1, BRDN0000399250
- Derived from:
- ccsbBroadEn_14896
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SMARCA5 (8467)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_14896
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8467 | SMARCA5 | SWI/SNF related, matrix ass... | NM_003601.4 | 99.9% | 100% | 2424T>C |
| 2 | human | 8467 | SMARCA5 | SWI/SNF related, matrix ass... | XR_001741338.1 | 63.3% | (many diffs) | |
| 3 | mouse | 93762 | Smarca5 | SWI/SNF related, matrix ass... | NM_053124.2 | 91.8% | 97.6% | (many diffs) |
| 4 | mouse | 545700 | Smarca5-ps | SWI/SNF related, matrix ass... | NR_002888.2 | 74.5% | (many diffs) | |
| 5 | mouse | 100862470 | LOC100862470 | SWI/SNF-related matrix-asso... | XM_017318994.1 | 29.5% | 30.6% | (many diffs) |
| 6 | mouse | 627585 | Gm13034 | predicted gene 13034; SWI/S... | NR_030771.1 | 24.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 3222
- ORF length:
- 3156
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgtc gtccgcggcc gagcctccgc cacccccgcc tcccgagagc gcgccttcca 121 agcccgcagc ctcgatcgcc agcggcggga gcaacagcag caacaaaggc ggccccgaag 181 gcgtcgcggc gcaggcggtt gcgtctgcgg ccagcgctgg tcccgcagac gccgagatgg 241 aggaaatatt tgatgatgcg tcacctggaa agcaaaagga aatccaagaa ccagatccta 301 cctatgaaga aaaaatgcaa actgaccggg caaatagatt cgagtattta ttaaagcaga 361 cagaactttt tgcacatttc attcaacctg ctgctcagaa gactccaact tcacctttga 421 agatgaaacc agggcgccca cgaataaaaa aagatgagaa gcagaactta ctatccgttg 481 gcgattaccg acaccgtaga acagagcaag aggaggatga agagctatta acagaaagct 541 ccaaagcaac caatgtttgc actcgatttg aagactctcc atcgtatgta aaatggggta 601 aactgagaga ttatcaggtc cgaggattaa actggctcat ttctttgtat gagaatggca 661 tcaatggtat ccttgcagat gaaatgggcc taggaaagac tcttcaaaca atttctcttc 721 ttgggtacat gaaacattat agaaacattc ctgggcctca tatggttttg gttcctaagt 781 ctacattaca caactggatg agtgaattca agagatgggt accaacactt agatctgttt 841 gtttgatagg agataaagaa caaagagctg cttttgtcag agacgtttta ttaccgggag 901 aatgggatgt atgtgtaaca tcttatgaaa tgcttattaa agagaagtct gtgttcaaaa 961 aatttaattg gagatactta gtaatagatg aagctcacag gatcaaaaat gaaaaatcta 1021 agttgtcaga aatagtgagg gaattcaaga ctacaaatag actattatta actggaacac 1081 ctcttcagaa caacttgcat gagctgtggt cacttcttaa ctttctgttg ccagatgtgt 1141 ttaattcagc agatgacttt gattcctggt ttgatacaaa caactgcctt ggggatcaaa 1201 aactagttga gaggcttcat atggttttgc gtccattcct ccttcgtcga attaaggctg 1261 atgttgaaaa gagtttgcct ccaaagaagg aagtaaaaat ctatgtgggc ctcagcaaaa 1321 tgcaaaggga atggtatact cggatattaa tgaaggatat agatatactc aactcagcag 1381 gcaagatgga caaaatgagg ttattgaaca tcctaatgca gttgagaaaa tgttgtaatc 1441 atccatatct ctttgatgga gcagaacctg gtccacctta tacaacagat atgcatctag 1501 taaccaacag tggcaaaatg gtggttttag acaagctgct ccctaagtta aaagaacaag 1561 gttcacgagt actaatcttc agtcaaatga caagggtatt ggacattttg gaagattatt 1621 gcatgtggag aaattatgag tactgcaggt tggatggtca gacaccccat gatgagagac 1681 aagactccat caatgcatac aatgaaccaa acagcacaaa gtttgttttc atgttaagca 1741 cgcgtgctgg tggtcttggc atcaatcttg cgactgctga tgtagtaatt ttgtatgatt 1801 ctgattggaa tccccaagta gatcttcagg ctatggaccg agcacataga attgggcaga 1861 ctaagacagt cagagtgttc cgctttataa ctgataacac tgtagaagaa agaatagtag 1921 aacgtgctga gatgaaactc agactggatt caatagtcat tcaacaaggg aggcttgtgg 1981 atcagaatct gaacaaaatt gggaaagatg aaatgcttca aatgattaga catggagcaa 2041 cacatgtgtt tgcttcaaag gaaagtgaga tcactgatga agatatcgat ggtattttgg 2101 aaagaggtgc aaagaagact gcagagatga atgaaaagct ctccaagatg ggcgaaagtt 2161 cacttagaaa ctttacaatg gatacagagt caagtgttta taacttcgaa ggagaagact 2221 atagagaaaa acaaaagatt gcattcacag agtggattga accacctaaa cgagaaagaa 2281 aagccaacta tgccgttgat gcatatttca gggaagctct tcgtgttagt gaacctaaag 2341 cacccaaggc tcctcgacct ccaaaacaac ccaatgttca ggatttccag ttctttcctc 2401 cacgtttatt tgaattactg gaaaaagaaa ttctgtttta cagaaaaact attgggtaca 2461 aggtacctcg aaatcctgag ctgcctaacg cagcacaggc acaaaaagaa gaacagctta 2521 aaattgatga agctgaatcc cttaatgatg aagagttaga ggaaaaagag aagcttctaa 2581 cacagggatt taccaattgg aataagagag attttaacca gtttatcaaa gctaatgaga 2641 agtggggtcg tgatgatatt gaaaatatag caagagaagt agaaggcaaa actccagaag 2701 aagtcattga atattcagct gtgttttggg aaaggtgcaa cgagctccag gacatagaga 2761 agattatggc tcagattgaa aggggagagg cgagaattca aagaagaata agcatcaaga 2821 aagcacttga cacaaagatt ggacggtaca aagcaccttt tcatcagctg agaatatcat 2881 atggtactaa caaaggaaaa aactatactg aagaagaaga tcgttttctg atttgtatgc 2941 ttcacaaact tggatttgac aaagaaaatg tttatgatga attgcgacag tgtattcgca 3001 actctcctca gttcagattt gactggtttc ttaagtccag aactgcaatg gagctccaga 3061 ggagatgtaa taccttaatt actttgattg aaagagaaaa catggaacta gaagaaaagg 3121 agaaggcaga gaaaaagaaa cgaggaccaa agccttcaac acagaaacgt aaaatggatg 3181 gcgcacctga tggtcgagga agaaaaaaga agctgaaact atgcccaact ttcttgtaca 3241 aagtggttgg taagcctatc cctaaccctc tcctcggtct cgattctacg tagtaatgag 3301 ctagcgctaa ccggtggcgc gttaagtcga caatcaacct ctggatta