Construct: ORF ccsbBroad304_14924
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012620.2_s304c1, BRDN0000459499
- Derived from:
- ccsbBroadEn_14924
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PAPSS2 (9060)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_14924
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9060 | PAPSS2 | 3'-phosphoadenosine 5'-phos... | NM_004670.4 | 100% | 100% | |
| 2 | human | 9060 | PAPSS2 | 3'-phosphoadenosine 5'-phos... | NM_001015880.2 | 99.1% | 99.1% | 864_878del |
| 3 | mouse | 23972 | Papss2 | 3'-phosphoadenosine 5'-phos... | NM_001201470.1 | 86.6% | 93.1% | (many diffs) |
| 4 | mouse | 23972 | Papss2 | 3'-phosphoadenosine 5'-phos... | XM_006527065.2 | 86.1% | 91.8% | (many diffs) |
| 5 | mouse | 23972 | Papss2 | 3'-phosphoadenosine 5'-phos... | XM_006527064.3 | 85.9% | 92.4% | (many diffs) |
| 6 | mouse | 23972 | Papss2 | 3'-phosphoadenosine 5'-phos... | NM_011864.3 | 85.5% | 91.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1908
- ORF length:
- 1842
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgtc ggggatcaag aagcaaaaga cggagaacca gcagaaatcc accaatgtag 121 tctatcaggc ccaccatgtg agcaggaata agagagggca agtggttgga acaaggggtg 181 ggttccgagg atgtaccgtg tggctaacag gtctctctgg tgctggaaaa acaacgataa 241 gttttgccct ggaggagtac cttgtctccc atgccatccc ttgttactcc ctggatgggg 301 acaatgtccg tcatggcctt aacagaaatc tcggattctc tcctggggac agagaggaaa 361 atatccgccg gattgctgag gtggctaagc tgtttgctga tgctggtctg gtctgcatta 421 ccagctttat ttctccattc gcaaaggatc gtgagaatgc ccgcaaaata catgaatcag 481 cagggctgcc attctttgaa atatttgtag atgcacctct aaatatttgt gaaagcagag 541 acgtaaaagg cctctataaa agggccagag ctggggagat taaaggattt acaggtattg 601 attctgatta tgagaaacct gaaactcctg agcgtgtgct taaaaccaat ttgtccacag 661 tgagtgactg tgtccaccag gtagtggaac ttctgcaaga gcagaacatt gtaccctata 721 ctataatcaa agatatccac gaactctttg tgccggaaaa caaacttgac cacgtccgag 781 ctgaggctga aactctccct tcattatcaa ttactaagct ggatctccag tgggtccagg 841 ttttgagcga aggctgggcc actcccctca aaggtttcat gcgggagaag gagtacttac 901 aggttatgca ctttgacacc ctgctagatg atggcgtgat caacatgagc atccccattg 961 tactgcccgt ctctgcagag gataagacac ggctggaagg gtgcagcaag tttgtcctgg 1021 cacatggtgg acggagggta gctatcttac gagacgctga attctatgaa cacagaaaag 1081 aggaacgctg ttcccgtgtt tgggggacaa catgtacaaa acacccccat atcaaaatgg 1141 tgatggaaag tggggactgg ctggttggtg gagaccttca ggtgctggag aaaataagat 1201 ggaatgatgg gctggaccaa taccgtctga cacctctgga gctcaaacag aaatgtaaag 1261 aaatgaatgc tgatgcggtg tttgcattcc agttgcgcaa tcctgtccac aatggccatg 1321 ccctgttgat gcaggacact cgccgcaggc tcctagagag gggctacaag cacccggtcc 1381 tcctactaca ccctctgggc ggctggacca aggatgacga tgtgcctcta gactggcgga 1441 tgaagcagca cgcggctgtg ctcgaggaag gggtcctgga tcccaagtca accattgttg 1501 ccatctttcc gtctcccatg ttatatgctg gccccacaga ggtccagtgg cactgcaggt 1561 cccggatgat tgcgggtgcc aatttctaca ttgtggggag ggaccctgca ggaatgcccc 1621 atcctgaaac caagaaggat ctgtatgaac ccactcatgg gggcaaggtc ttgagcatgg 1681 cccctggcct cacctctgtg gaaatcattc cattccgagt ggctgcctac aacaaagcca 1741 aaaaagccat ggacttctat gatccagcaa ggcacaatga gtttgacttc atctcaggaa 1801 ctcgaatgag gaagctcgcc cgggaaggag agaatccccc agatggcttc atggccccca 1861 aagcatggaa ggtcctgaca gattattaca ggtccctgga gaagaactac ccaactttct 1921 tgtacaaagt ggttggtaag cctatcccta accctctcct cggtctcgat tctacgtagt 1981 aatgagctag cgctaaccgg tggcgcgtta agtcgacaat caacctctgg atta