Construct: ORF ccsbBroad304_15029

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF000116.1_s304c1, BRDN0000405246
Derived from:
ccsbBroadEn_15029
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
MYRIP (25924)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_15029
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 25924 MYRIP myosin VIIA and Rab interac... NM_001284425.1 99.7% 99.6% (many diffs)
2 human 25924 MYRIP myosin VIIA and Rab interac... NM_001284423.1 89.4% 82.4% (many diffs)
3 human 25924 MYRIP myosin VIIA and Rab interac... NM_015460.4 89.4% 82.4% (many diffs)
4 human 25924 MYRIP myosin VIIA and Rab interac... XM_011533575.1 89.4% 82.4% (many diffs)
5 human 25924 MYRIP myosin VIIA and Rab interac... NM_001284426.1 87% 87.1% (many diffs)
6 human 25924 MYRIP myosin VIIA and Rab interac... NM_001284424.2 81.8% 74.9% (many diffs)
7 human 25924 MYRIP myosin VIIA and Rab interac... NR_104316.2 44.6% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
2379
ORF length:
2310
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat gaggaaggca gcaagtggaa catcctctcg aagcaccagc agtttgtgga
121gcactgctgc atgcgctgct gctcgccctt caccttcctc gtcaacacca agcgccagtg
181tggagattgc aaattcaatg tctgcaagag ctgctgctcc taccagaagc acgaaaaggc
241ctgggtctgc tgcgtctgcc agcaagcgag gaggaagcct ttttgagtca aacctggaga
301atgaaggaag catttctggc agtgattcaa cattttatag gcagtcagaa ggacatagtg
361tgatggacac cttggctgtg gccctacggg tggctgaaga ggccattgag gaagcaattt
421ccaaagcaga ggcatatggg gacagcctgg acaagcaaaa tgaggccagt tacctgcggg
481accacaagga ggagctaact gaggaactgg ccacgacaat cctgcagaag attatacgaa
541aacagaagag caaaagtagg cagcaagtgg aagaagagcc aggatggcca catccccaga
601gttgcagcac aaaggtggca gatgagggga cctcagcatc ccctggaggc taccgtgctc
661ccgctgccct ctggaggtcc cagtctgcct tctcaatcac tggagaagaa gccctgaaga
721cccctccagt ggaggctcca tcgaggcagc caagggacca aggccaacac ccgagagcag
781agtctgctct gcccagctgg aagagtgtgg acaggctgga tgaaacaaac ctggccccag
841ttttgcagag ccccgacggg aactgggtgg ccctgaagga tggcgctcca ccccccaccc
901gactactggc caaacctaag agcgggacgt ttcaggccct ggaggtggcc tccagtgtgg
961catctgccta cgatgagatg ggctccgata gcgaggaaga ctttgactgg agtgaggcct
1021tgagcaagct gtgtcccagg tcccgggccc tgcccaggaa cccccagcct cagcccacac
1081aggcccagag ctctgaccaa ggccccatag ctgcctcccc atcctctgca ctctccccca
1141accctgaggc catgtgctct gactcggaga cctcctccgc aggctcttcc cgagaagttg
1201ggcaccaggc cagactgtcc tggttgcaga ggaaggcccc caggaaccct gcagctgaga
1261agatgcgctt gcatggagag ctggacgtga acttcaaccc ccagttggcc agcagggaga
1321cctcggacag cagcgagccg gaggaggccc cccacaccac agaccggcgg gccaggaggt
1381ggagaagagc ccgattgggc tcagaagagc caagcaaaga accatcttcc cccagcgccc
1441agctccggga tctagacaca catcaggtgt cggatgattt atcagagaca gacatcagca
1501atgaggctcg ggatccccag actctcacag acaccacaga ggagaaacgg agaaacaggc
1561tgtacgagtt agcaatgaaa atgagtgaaa aggagacttc ttcaggggag gatcaggagt
1621ctgagcccaa gacagaatct gagaaccaga aggaaagtct gtcctctgaa gacaacagcc
1681agagtgtcca ggaagagctg aagaagaagt tttctgctgt ttctctctgc aacatctcca
1741cagaagtcct gaaagtcatc aatgccacag aggagttgat agcaggatct acagggccct
1801gggagtcccc acaagtccct cctgacagac agaaggggat gtttcctcgt gggacagacc
1861aagtgagact ggatgagcag ctgacttccc tggaagaaaa tgtatacctg gcagcaggca
1921ctgtgtatgg actggagacc cagctgactg agctagaaga tgccgcccgc tgcatccaca
1981gcggcactga tgagacccat ctggcggatc tggaggacca ggtggccacg gctgcagccc
2041aagtccacca tgctgaactc cagatttcag atattgagag ccggatttca gccctgacca
2101ttgcaggatt aaacatagca ccatgtgtgc gcttcacaag aagacgggat cagaagcaaa
2161ggacccaggt acaaaccata gatacatcaa ggcagcaaag gaggaaactg cctgctccac
2221cggtgaaagc tgaaaaaatt gagacatctt cagtgactac cattaaaaca tttaaccaca
2281acttcattct ccaaggctcc tcaacaaaca ggactaagga aaggaaaggc accaccaagg
2341atttgatgga gcctgctctg gagtcagctg tgatgtactt gccaactttc ttgtacaaag
2401tggttggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag taatgagcta
2461gcgctaaccg gtggcgcgtt aagtcgacaa tcaacctctg gatta

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