Construct: ORF ccsbBroad304_15061
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006250.1_s304c1, BRDN0000459604
- Derived from:
- ccsbBroadEn_15061
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- VRK3 (51231)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_15061
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51231 | VRK3 | VRK serine/threonine kinase 3 | NM_001308420.2 | 100% | 100% | |
| 2 | human | 51231 | VRK3 | VRK serine/threonine kinase 3 | NM_016440.4 | 86.6% | 84.9% | (many diffs) |
| 3 | human | 51231 | VRK3 | VRK serine/threonine kinase 3 | XM_005258971.4 | 86.6% | 84.9% | (many diffs) |
| 4 | human | 51231 | VRK3 | VRK serine/threonine kinase 3 | XM_006723237.3 | 86.6% | 84.9% | (many diffs) |
| 5 | human | 51231 | VRK3 | VRK serine/threonine kinase 3 | NM_001025778.1 | 76% | 74.5% | (many diffs) |
| 6 | human | 51231 | VRK3 | VRK serine/threonine kinase 3 | XM_005258972.4 | 76% | 74.5% | (many diffs) |
| 7 | human | 51231 | VRK3 | VRK serine/threonine kinase 3 | XM_006723238.4 | 76% | 74.5% | (many diffs) |
| 8 | human | 51231 | VRK3 | VRK serine/threonine kinase 3 | XR_001753695.2 | 55.8% | 1_91del;1328_2215del | |
| 9 | human | 51231 | VRK3 | VRK serine/threonine kinase 3 | XR_001753696.1 | 53.6% | 1_181del;1418_2305del | |
| 10 | human | 51231 | VRK3 | VRK serine/threonine kinase 3 | XR_001753698.2 | 52.7% | 1_91del;702_703ins67;1261_2148del | |
| 11 | human | 51231 | VRK3 | VRK serine/threonine kinase 3 | XR_001753697.2 | 45.6% | 1_254del;390_391ins150;1341_2228del | |
| 12 | human | 51231 | VRK3 | VRK serine/threonine kinase 3 | XR_002958320.1 | 40.9% | 1_91del;1328_3019del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1302
- ORF length:
- 1236
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgat ctccttctgt ccagactgtg gcaaaagtat ccaagcggca ttcaaattct 121 gcccctactg tggaaattct ttgcctgtag aggagcatgt agggtcccag acctttgtca 181 atccacatgt gtcatccttc caaggctcaa agagagggct gaactccagt tttgaaacct 241 ctcctaagaa agtgaaatgg tccagcaccg tcacctctcc ccgattatcc ctcttctcag 301 atggtgacag ttctgagtct gaagatactc tgagttcctc tgagagatcc aaaggctccg 361 ggagcagacc cccaaccccc aaaagcagcc ctcagaagac caggaagagc cctcaggtga 421 ccaggggtag ccctcagaag accagctgta gccctcagaa gaccaggcag agccctcaga 481 cgctgaagcg gagccgagtg accacctcac ttgaagcttt gcccacaggg acagtgctga 541 cagacaagag tgggcgacag tggaagctga agtccttcca gaccagggac aaccagggca 601 ttctctatga agctgcaccc acctccaccc tcacctgtga ctcaggacca cagaagcaaa 661 agttctcact caaactggat gccaaggatg ggcgcttgtt caatgagcag aacttcttcc 721 agcgggccgc caagcctctg caagtcaaca agtggaagaa gctgtactcg accccactgc 781 tggccatccc tacctgcatg ggtttcggtg ttcaccagga caaatacagg ttcttggtgt 841 tacccagcct ggggaggagc cttcagtcgg ccctggatgt cagcccaaag catgtgctgt 901 cagagaggtc tgtgctgcag gtggcctgcc ggctgctgga tgccctggag ttcctccatg 961 agaatgagta tgttcatgga aatgtgacag ctgaaaatat ctttgtggat ccagaggacc 1021 agagtcaggt gactttggca ggctatggct tcgccttccg ctattgccca agtggcaaac 1081 acgtggccta cgtggaaggc agcaggagcc ctcacgaggg ggaccttgag ttcattagca 1141 tggacctgca caagggatgc gggccctccc gccgcagcga cctccagagc ctgggctact 1201 gcatgctgaa gtggctctac gggtttctgc catggacaaa ttgccttccc aacactgagg 1261 acatcatgaa gcaaaaacag aagttgcctt gggattcatt ttacccaact ttcttgtaca 1321 aagtggttgg taagcctatc cctaaccctc tcctcggtct cgattctacg tagtaatgag 1381 ctagcgctaa ccggtggcgc gttaagtcga caatcaacct ctggatta