Construct: ORF ccsbBroad304_15138

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF018940.1_s304c1, BRDN0000459424
Derived from:
ccsbBroadEn_15138
DNA Barcode:
None
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Early stop codon detected

Originally Annotated References:

Gene:
PINK1 (65018)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_15138
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 65018 PINK1 PTEN induced kinase 1 NM_032409.3 96.8% 32.2% (many diffs)
2 human 102464833 MIR6084 microRNA 6084 NR_106732.1 6.5% 0_1ins82;110_111ins1500
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
906
ORF length:
837
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat ggcggtgcga caggcgctgg gccgcggcct gcagctgggt cgagcgctgc
121tgctgcgctt cacgggctgt gtccgcgggg agcgtccagg ctgggccgca ggaccgggcg
181cggagcctcg cagggtcggg ctcgggctcc ctaaccgtct ccgcttcttc cgccagtcgg
241tggccgggct ggcggcgcgg ttgcagcggc agttcgtggt gcgggcctgg ggctgcgcgg
301gcccttgcgg ccgggcagtc tttctggcct tcgggctagg gctgggcctc atcgaggaaa
361aacaggcgga gagccggcgg gcggtctcgg cctgtcagga gatccaggca atttttaccc
421agaaaagcaa gccggggcct gacccgttgg acacgagacg cttgcagggc tttcggctgg
481aggagtatct gatagggcag tccattggta agggctgcag tgctgctgtg tatgaagcca
541ccatgcctac attgccccag aacctggagg tgacaaagag caccgggttg cttccaggga
601gaggcccagt accagtgcac cagagaaggg caggagcgag ctccgggggc ccctgccttc
661cccttggcca tcaagatgat gtggaacatc tcggcaggtt cctccagcga agccatcttg
721aacacaatga gccaggagct ggtcccagcg agccgagtgg ccttggcttg agggtatgga
781gcagtcactt acagaaaatc caagagaggt cccaagcaac tagcccctca ccccaacatc
841atccgggttc tccgcgcctt cacctcttcc gtgccgctgc tgccaggggc cctggtcgac
901taccctgatg tgctgccctc acgcctccac cctgaaggcc tgggccatgg ccggacgctg
961ttcctcgtta tgaagaacta tccctgtacc ctgcgccagt acctttgtgt gaacacaccc
1021agcccccgcc tcggcgccat gatgctgctg cagctgctgg aaggcgtgga ccatctggtt
1081caacagggca tcgcgcacag agactgaaat ccgacaacat ccttgtggag ctggacccag
1141acggctgccc ctggctggtg atcgcagatt ttggctgctg cctggctgat gagagcatcg
1201gcctgcagtt gcccttcagc agctggtacg tggatcgggg cggaaacggc tgtctgatgg
1261ccccagaggt gtccacggcc cgtcctggcc ccagggcagt gattgactac agcaaggctg
1321atgcctgggc agtgggagcc atcgcctatg aaatcttcgg gcttgtcaat cccttctacg
1381gccagggcaa ggcccacctt gaaagccgca gctaccaaga ggctcagcta cctgcactgc
1441ccgagtcagt gcctccagac gtgagacagt tggtgagggc actgctccag cgagaggcca
1501gcaagagacc atctgcccga gtagccgcaa atgtgcttca tctaagcctc tggggtgaac
1561atattctagc cctgaagaat ctgaagttag acaagatggt tggctggctc ctccaacaat
1621cggccgccac tttgttggcc aacaggctca cagagaagtg ttgtgtggaa acaaaaatga
1681agatgctctt tctggctaac ctggagtgtg aaacgctctg ccaggcagcc ctcctcctct
1741gctcatggag ggcagccctg ttgccaactt tcttgtacaa agtggttggt aagcctatcc
1801ctaaccctct cctcggtctc gattctacgt agtaatgagc tagcgctaac cggtggcgcg
1861ttaagtcgac aatcaacctc tggatta

Download FASTA (ORF) (Full)