Construct: ORF ccsbBroad304_15151
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018301.1_s304c1, BRDN0000404866
- Derived from:
- ccsbBroadEn_15151
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MICALL2 (79778)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_15151
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79778 | MICALL2 | MICAL like 2 | NM_182924.4 | 99.7% | 99.6% | (many diffs) |
| 2 | human | 79778 | MICALL2 | MICAL like 2 | XR_926947.1 | 85.2% | (many diffs) | |
| 3 | human | 79778 | MICALL2 | MICAL like 2 | XR_001744875.1 | 84.2% | (many diffs) | |
| 4 | human | 79778 | MICALL2 | MICAL like 2 | XM_011515520.3 | 84.1% | 82.8% | (many diffs) |
| 5 | human | 79778 | MICALL2 | MICAL like 2 | XM_011515522.3 | 82.8% | 82.3% | (many diffs) |
| 6 | human | 79778 | MICALL2 | MICAL like 2 | XR_002956487.1 | 80.6% | (many diffs) | |
| 7 | human | 79778 | MICALL2 | MICAL like 2 | XM_024446932.1 | 76.4% | 75% | (many diffs) |
| 8 | human | 79778 | MICALL2 | MICAL like 2 | XM_011515524.2 | 30.9% | 29.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2778
- ORF length:
- 2712
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc ggccatcagg gcgctgcaac agtggtgccg gcagcagtgc gagggctacc 121 gcgacgtgaa tatctgcaac atgaccacgt cgttccgcga cggcctggct ttctgcgcca 181 tcctgcaccg ccaccggccc gacctcataa acttcagtgc tctcaagaag gaaaatattt 241 atgaaaacaa taaactggcc ttccgcgtgg ccgaggagca cttgggcatc ccagccttgc 301 tggatgccga ggacatggtg gccttgaagg tgcctgaccg gctgagcatc ttgacctacg 361 tgtcccagta ttacaactac ttccacggcc gctcccccat tgggggcatg gcaggcgtga 421 agagggcctc ggaggactct gaggaggagc cgtcagggaa gaaggctcca gtccaggcgg 481 ccaagctgcc ctcgcccgcc ccagcccgga agcctccact atctccagcc cagacaaacc 541 ctgtggtcca gaggaggaat gagggtgcag ggggcccgcc ccccaagact gaccaggcat 601 tggcgggcag cttggtcagc agcacctgcg gggtctgcgg caagcacgtg cacctggtac 661 agcggcacct ggccgacggg aggctttacc accggagctg cttcaggtgt aagcagtgct 721 cctgcacgct gcactcgggg gcctacaagg ccacaggaga gccgggcacc ttcgtctgca 781 ccagccacct ccccgcagcc gcctctgcaa gccccaagtt gacgggtctg gtcccccgac 841 agccaggggc catgggtgtg gattccagga cctcctgttc cccacagaag gcccaggagg 901 caaacaaggc cagaccgtcg gcctgggagc ctgctgcggg caactcgcct gccagggctt 961 ccgttccagc tgcacccaac cctgcagcca ccagcgccac gtccgtccac gtgaggagcc 1021 cagccaggcc ctctgagagc cgcctggccc ccactcccac ggaggggaaa gtccgccctc 1081 gtgtgaccaa tagctccccg atgggctggt cgtcagctgc cccgtgcaca gcagcggctg 1141 cctcccatcc cgccgtgccc ccgagtgccc cagaccctcg cccggccaca ccccagggcg 1201 ggggagcccc ccgagtggca gctcctcaaa ccacactcag ttcaagctcc acatctgcag 1261 ccacggtgga ccccccagcc tggaccccgt ccgcctccag gacccagcag gcccggaata 1321 agtttttcca aacatcagca gtgccccccg gcaccagcct ttctggcaga ggtcccaccc 1381 cgtcacttgt tctatccaag gacagcagca aggagcaggc gcggaacttc ctcaagcagc 1441 ccctcccagc gctggaagag gctggcgctc cggcgcctgg caggccctcc ccagccactg 1501 ccgctgttcc cagttctcag cccaaaactg aagcaccaca agcaagtccc ttagccaagc 1561 cgttacagtc ctcgtctccc cgggtgcttg gcctcccttc gaggatggaa ccgccagccc 1621 cgctgagcac gagcagtacc tctcaggcat ccgcgttgcc cccggcaggc aggaggaact 1681 tggctgaatc ctcaggggtc ggcagggtgg gtgctggctc caggccgaag ccagaggccc 1741 cgatggcaaa gggtaaaagc accaccttaa cgcaggacat gagcaccagc ctccaggaag 1801 gccaggagga cgggccggca ggatggagag cgaatctgaa gcccgtggac aggagaagcc 1861 cagctgagag gactctgaag cccaaggaac cacgggccct ggcagagccg agggcggggg 1921 aggcccccag gaaggtctca ggcagctttg ctgggagtgt ccacatcacc ctgacccccg 1981 tgaggcctga caggacccca cgcccagcca gcccaggacc cagcctccca gccaggtccc 2041 cctccccacc ccgccgcagg agactggccg tccctgccag cctcgacgtt tgtgacaact 2101 ggcttcggcc ggagccccct ggccaggaag cccgagtgca gagctggaag gaggaggaga 2161 agaaacctca ccttcagggc aaaccaggga gacccttgtc cccggccaat gtccctgctc 2221 tgcctggcga gacggtgacc tccccagtca ggctgcaccc cgactacctc tccccggagg 2281 agatacagag gcagctgcag gacatcgaga ggcggctgga cgccctggag ctccgcggcg 2341 tggagctgga gaagcgactg cgggcggccg agggagatga cgctgaggat agcctcatgg 2401 tggactggtt ctggctcatt cacgagaagc agcttctgct gagacaggag tcagagctga 2461 tgtacaagtc caaggcccag cgtctggagg agcagcagct ggacatcgag ggcgagctgc 2521 gccggctcat ggccaagccc gaggctctga agtcactgca ggagcggcgg cgggagcagg 2581 agctgctgga gcagtacgtg agcaccgtga acgaccgcag tgacatcgtg gactcgctgg 2641 acgaggaccg gctccgggaa caagaggagg atcagatgct gcgggacatg attgagaagc 2701 tgggcctcca gaggaagaag tccaagttcc gcttgtccaa gatctggtca ccaaaaagct 2761 caagcagccc ctcccagtac ccaactttct tgtacaaagt ggttggtaag cctatcccta 2821 accctctcct cggtctcgat tctacgtagt aatgagctag cgctaaccgg tggcgcgtta 2881 agtcgacaat caacctctgg atta