Transcript: Human XR_002956487.1

PREDICTED: Homo sapiens MICAL like 2 (MICALL2), transcript variant X4, misc_RNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
MICALL2 (79778)
Length:
2875
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
XR_002956487.1
NBCI Gene record:
MICALL2 (79778)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XR_002956487.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000155440 CGACGTGAATATCTGCAACAT pLKO.1 228 3UTR 100% 4.950 6.930 N MICALL2 n/a
2 TRCN0000158193 CCCGTCACTTGTTCTATCCAA pLKO.1 1485 3UTR 100% 3.000 4.200 N MICALL2 n/a
3 TRCN0000156495 CTCAGTTCAAGCTCCACATCT pLKO.1 1342 3UTR 100% 4.950 3.465 N MICALL2 n/a
4 TRCN0000158030 CACTCAGTTCAAGCTCCACAT pLKO.1 1340 3UTR 100% 4.050 2.835 N MICALL2 n/a
5 TRCN0000157193 GCGACGTGAATATCTGCAACA pLKO.1 227 3UTR 100% 4.050 2.835 N MICALL2 n/a
6 TRCN0000157130 GACGTTTGTGACAACTGGCTT pLKO.1 2187 3UTR 100% 2.640 1.848 N MICALL2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XR_002956487.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_15151 pDONR223 52% 80.6% None (many diffs) n/a
2 ccsbBroad304_15151 pLX_304 0% 80.6% V5 (many diffs) n/a
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