Construct: ORF ccsbBroad304_15222
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017213.1_s304c1, BRDN0000399161
- Derived from:
- ccsbBroadEn_15222
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FAM83F (113828)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_15222
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 113828 | FAM83F | family with sequence simila... | NM_138435.4 | 100% | 100% |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1566
- ORF length:
- 1500
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc cgagtcccag ctgaactgcc tggacgaggc gcacgtgaac gagaaggtga 121 ccgaggcgca ggccgccttc tactactgcg agcggcggcg ggccgcgctg gaggcgctgc 181 tgggcggcgg cgagcaggcc taccgcgagc ggctcaagga ggagcagctg cgggacttcc 241 tctccagccc ggagcgccag gccctgcggg ccgcctggag cccctacgag gacgccgtcc 301 ccgccgccaa cgcccggggc aagagcaagg ccaaggccaa ggcccccgcg ccggcgccgg 361 ctgagtccgg cgagtccctg gcctactggc ccgaccgttc cgacaccgag gtgcctcctc 421 tggacctggg ctggacggac actggtttct accgcggcgt gagccgggtc acgctcttca 481 cccacccgcc caaggacgag aaggcgccgc acctcaagca ggtggtcagg cagatgatcc 541 aacaggccca gaaggtcatt gctgtggtca tggacctctt cactgatggt gatatctttc 601 aagacattgt ggatgctgcc tgtaagcgcc gggtcccagt gtacatcatc ctggacgagg 661 caggagtgaa gtatttcctg gagatgtgtc aggacctgca gctcactgac ttccggattc 721 ggaacatccg tgtccgctct gtgacaggcg tcggcttcta catgcccatg gggaggatca 781 aggggaccct gtcatcaagg ttcctgatgg tggacggtga caaagtggcc actggatctt 841 acaggttcac ctggagttcc tcccatgtgg acagaaacct cctcctgctc ctgacaggac 901 agaacgtaga gccctttgac acggagttcc gggagctgta cgccatctcc gaggaggtgg 961 acttgtaccg gcagctgagc ctggcgggca gggttggcct ccattactcc tccactgtgg 1021 ctcgaaagct tatcaacccc aagtacgcct tggtgtcagg ctgccgccac ccgcctgggg 1081 agatgatgcg ctgggctgcc cggcaacagc gggaggcggg cggcaacccg gaggggcagg 1141 aggagggcgc cagcggtggc gagtcggcct ggcgcctgga gagcttcctg aaagacctgg 1201 ttacggtgga gcaggtgctg ccccccgtgg agcccatccc cttgggagag ctgagccaga 1261 aggatggcag gatggtctct cacatgcaca gagacctgaa gcccaaatcc cgagaggcac 1321 ccagccgaaa cggcatggga gaagcggccc ggggggaggc cgcccccgcc aggcgcttca 1381 gcagcaggct cttcagtcgc cgagccaaga ggcctgcggc gcccaatggc atggccagct 1441 ctgtctccac cgagacctct gaagtggagt ttctgacggg gaagaggccc aacgagaatt 1501 ccagtgctga catctcaggt aaaacaagtc ccagttctgc caagcctagc aactgtgtga 1561 tttcctgccc aactttcttg tacaaagtgg ttggtaagcc tatccctaac cctctcctcg 1621 gtctcgattc tacgtagtaa tgagctagcg ctaaccggtg gcgcgttaag tcgacaatca 1681 acctctggat ta