Transcript: Human NM_138435.4

Homo sapiens family with sequence similarity 83 member F (FAM83F), mRNA.

Source:
NCBI, updated 2019-09-28
Taxon:
Homo sapiens (human)
Gene:
FAM83F (113828)
Length:
15561
CDS:
90..1592

Additional Resources:

NCBI RefSeq record:
NM_138435.4
NBCI Gene record:
FAM83F (113828)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_138435.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000438014 AGCTCACTGACTTCCGGATTC pLKO_005 724 CDS 100% 6.000 8.400 N FAM83F n/a
2 TRCN0000166042 CATTGTGGATGCTGCCTGTAA pLKO.1 629 CDS 100% 4.950 6.930 N FAM83F n/a
3 TRCN0000166319 CGAGGCAGGAGTGAAGTATTT pLKO.1 680 CDS 100% 13.200 10.560 N FAM83F n/a
4 TRCN0000158941 GCCCATTCTTTAAACCTTTAT pLKO.1 1914 3UTR 100% 13.200 9.240 N FAM83F n/a
5 TRCN0000165961 GCCTAGCAACTGTGTGATTTC pLKO.1 1568 CDS 100% 10.800 7.560 N FAM83F n/a
6 TRCN0000437775 TGATGGTGGACGGTGACAAAG pLKO_005 829 CDS 100% 10.800 7.560 N FAM83F n/a
7 TRCN0000164692 CCACTGGATCTTACAGGTTCA pLKO.1 853 CDS 100% 4.050 2.835 N FAM83F n/a
8 TRCN0000434615 GTCGCACACTGCACCAGTTTG pLKO_005 1662 3UTR 100% 3.600 2.520 N FAM83F n/a
9 TRCN0000164967 GTCATCAAGGTTCCTGATGGT pLKO.1 815 CDS 100% 0.264 0.185 N FAM83F n/a
10 TRCN0000162865 GCCTCCTTGTTTACATGAAGT pLKO.1 1825 3UTR 100% 4.950 2.970 N FAM83F n/a
11 TRCN0000166231 CCTGTCATCAAGGTTCCTGAT pLKO.1 812 CDS 100% 4.050 2.430 N FAM83F n/a
12 TRCN0000439191 TGGTCAGGCAGATGATCCAAC pLKO_005 547 CDS 100% 4.050 2.430 N FAM83F n/a
13 TRCN0000165534 GAGACAGGGTTTCACCATGTT pLKO.1 4782 3UTR 100% 4.950 2.475 Y n/a
14 TRCN0000008902 CCTCCCAAAGTGTTGGGATTA pLKO.1 8311 3UTR 100% 1.080 0.540 Y GPR83 n/a
15 TRCN0000156315 CCTCCCAAAGTGTTGGGATTA pLKO.1 8311 3UTR 100% 1.080 0.540 Y MYORG n/a
16 TRCN0000155836 CCCAAAGTGCTGGGATTACAA pLKO.1 4853 3UTR 100% 5.625 2.813 Y KLHL30 n/a
17 TRCN0000264189 CAAGTAGCTGGGACTACAGGA pLKO_005 9869 3UTR 100% 2.640 1.320 Y LINC01098 n/a
18 TRCN0000141025 CCCAAAGTGCTGGGATTACTT pLKO.1 4853 3UTR 100% 5.625 2.813 Y EID2B n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_138435.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_15222 pDONR223 95.8% 100% 100% None n/a
2 ccsbBroad304_15222 pLX_304 0% 100% 100% V5 n/a
3 TRCN0000474086 CGGTTATGCTGGGTTACCATAATT pLX_317 34.5% 100% 100% V5 n/a
4 ccsbBroadEn_13027 pDONR223 100% 66.3% 66.2% None 1_504del;1045C>T n/a
5 ccsbBroad304_13027 pLX_304 0% 66.3% 66.2% V5 1_504del;1045C>T n/a
6 TRCN0000481573 CCTTATCCTGTAGTCACAAGGGGT pLX_317 49.2% 66.3% 66.2% V5 1_504del;1045C>T n/a
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