Construct: ORF ccsbBroad304_15309
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013378.1_s304c1, BRDN0000411612
- Derived from:
- ccsbBroadEn_15309
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- QRFP (347148)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_15309
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 347148 | QRFP | pyroglutamylated RFamide pe... | NM_198180.3 | 98.7% | 97.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 477
- ORF length:
- 408
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggtaaggcct taccccctga tctacttcct cttcctgccg ctgggcgcct 121 gcttccctct actggacaga agagagccca cagacgccat gggtggcctc ggagctggag 181 aacgctgggc cgacctggcc atggggcccc gaccccactc cgtgtggggt tcctctcggt 241 ggctgagagc ttcacagcca caggccctgc ttgtcatagc cagggggctg cagacatcgg 301 gcagagagca tgctggctgc agattccgct tcggtcggcg ggacgaaggc agtgaggcca 361 ccggcttcct ccctgctgcg ggggagaaga ccagcggccc gttagggaac ctggctgagg 421 agctcaatgg ctacagcagg aagaaaggcg gctgcagatt ccgcttcggt cggcggttgc 481 caactttctt gtacaaagtg gttggtaagc ctatccctaa ccctctcctc ggtctcgatt 541 ctacgtagta atgagctagc gctaaccggt ggcgcgttaa gtcgacaatc aacctctgga 601 tta