Construct: ORF ccsbBroad304_15332
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018786.1_s304c1, BRDN0000459271
- Derived from:
- ccsbBroadEn_15332
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CLK3 (1198)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_15332
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1198 | CLK3 | CDC like kinase 3 | NM_003992.4 | 11.3% | 11.2% | 1_1302del;1456C>T |
| 2 | human | 1198 | CLK3 | CDC like kinase 3 | NM_001130028.1 | 8.7% | 8.6% | 1_1746del;1900C>T |
| 3 | mouse | 102414 | Clk3 | CDC-like kinase 3 | XM_017313061.1 | 19% | 20% | (many diffs) |
| 4 | mouse | 102414 | Clk3 | CDC-like kinase 3 | XM_006510739.3 | 10.4% | 11% | (many diffs) |
| 5 | mouse | 102414 | Clk3 | CDC-like kinase 3 | XM_017313060.1 | 10.4% | 11% | (many diffs) |
| 6 | mouse | 102414 | Clk3 | CDC-like kinase 3 | NM_007713.4 | 8% | 8.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 237
- ORF length:
- 168
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gctccaagac tccctggagc acgtgcagct gtttgacctg atgaggagga 121 tgttagaatt tgaccctgcc cagcgcatca cactggccga ggccctgctg caccccttct 181 ttgctggcct gacccctgag gagcggtcct tccacaccag ctgcaaccca agcagattgc 241 taactttctt gtacaaagtg gttggtaagc ctatccctaa ccctctcctc ggtctcgatt 301 ctacgtagta atgagctagc gctaaccggt ggcgcgttaa gtcgacaatc aacctctgga 361 tta