Construct: ORF ccsbBroad304_15361

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF006584.1_s304c1, BRDN0000410636
Derived from:
ccsbBroadEn_15361
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
ASPH (444)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_15361
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 444 ASPH aspartate beta-hydroxylase NM_001164756.1 100% 100%
2 human 444 ASPH aspartate beta-hydroxylase XM_024447159.1 83.6% 83.2% (many diffs)
3 human 444 ASPH aspartate beta-hydroxylase NM_001164755.2 57.3% 42.1% (many diffs)
4 human 444 ASPH aspartate beta-hydroxylase NM_001164754.1 50.5% 41.6% (many diffs)
5 human 444 ASPH aspartate beta-hydroxylase NM_032466.4 47.7% 40.3% (many diffs)
6 human 444 ASPH aspartate beta-hydroxylase XM_024447157.1 43% 36.3% (many diffs)
7 human 444 ASPH aspartate beta-hydroxylase XM_017013438.1 24.7% 18.9% (many diffs)
8 human 444 ASPH aspartate beta-hydroxylase XM_005251243.2 24.6% 18.9% (many diffs)
9 human 444 ASPH aspartate beta-hydroxylase XM_005251247.2 24.6% 19.5% (many diffs)
10 human 444 ASPH aspartate beta-hydroxylase XM_017013432.1 23.8% 18.3% (many diffs)
11 human 444 ASPH aspartate beta-hydroxylase XM_017013430.1 23.8% 18.2% (many diffs)
12 human 444 ASPH aspartate beta-hydroxylase XM_024447155.1 23.7% 18.8% (many diffs)
13 human 444 ASPH aspartate beta-hydroxylase XM_017013434.1 23.1% 19.3% (many diffs)
14 human 444 ASPH aspartate beta-hydroxylase XM_005251239.2 23.1% 19.3% (many diffs)
15 human 444 ASPH aspartate beta-hydroxylase XM_005251246.3 22.6% 16.9% (many diffs)
16 human 444 ASPH aspartate beta-hydroxylase XM_005251236.2 22.6% 19.2% (many diffs)
17 human 444 ASPH aspartate beta-hydroxylase XM_005251235.2 22.5% 19.1% (many diffs)
18 human 444 ASPH aspartate beta-hydroxylase XM_017013425.1 22.3% 18.7% (many diffs)
19 human 444 ASPH aspartate beta-hydroxylase XM_017013421.1 22.2% 18.8% (many diffs)
20 human 444 ASPH aspartate beta-hydroxylase XM_017013420.1 21.8% 18.5% (many diffs)
21 human 444 ASPH aspartate beta-hydroxylase XM_017013437.2 21.8% 16.3% (many diffs)
22 human 444 ASPH aspartate beta-hydroxylase XM_017013419.1 21.8% 18.5% (many diffs)
23 human 444 ASPH aspartate beta-hydroxylase XM_005251242.3 21.1% 17.4% (many diffs)
24 human 444 ASPH aspartate beta-hydroxylase XM_017013429.2 20.7% 17.2% (many diffs)
25 human 444 ASPH aspartate beta-hydroxylase NM_004318.4 20.6% 17.2% (many diffs)
26 human 444 ASPH aspartate beta-hydroxylase XM_017013428.2 20.4% 16.8% (many diffs)
27 human 444 ASPH aspartate beta-hydroxylase XM_017013424.2 20% 16.6% (many diffs)
28 human 444 ASPH aspartate beta-hydroxylase XM_017013422.2 20% 16.6% (many diffs)
29 mouse 65973 Asph aspartate-beta-hydroxylase NM_001177856.1 88% 90% (many diffs)
30 mouse 65973 Asph aspartate-beta-hydroxylase XM_017320345.1 77.5% 80.2% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
675
ORF length:
609
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggc ccagcgtaag aatgccaaga gcagcggcaa cagcagcagc agcggctccg
121gcagcggtag cacgagtgcg ggcagcagca gccccggggc ccggagagag acaaagcatg
181gaggacacaa gaatgggagg aaaggcggac tctcaggaac ttcattcttc acgtggttta
241tggtgattgc attgctgggc gtctggacat ctgtagctgt cgtttggttt gatcttgttg
301actatgagga agttctagcc aaagcaaagg acttccgtta taacttatca gaggtgcttc
361aaggaaaact aggaatctat gatgctgatg gtgatggaga ttttgatgtg gatgatgcca
421aagttttatt aggcctgacc aaagatggca gtaatgaaaa tattgattct cttgaggaag
481tccttaatat tttagcagag gaaagttcag attggtttta tggtttcctc tcatttctct
541atgatataat gactcctttt gaaatgctag aagaagaaga agaagaaagc gaaaccgcag
601atggtgttga tggtacgtca cagaatgaag gggttcaggg aaagacttgt gtcatattgg
661atttacataa ccagtnncca actttcttgt acaaagtggt tggtaagcct atccctaacc
721ctctcctcgg tctcgattct acgtagtaat gagctagcgc taaccggtgg cgcgttaagt
781cgacaatcaa cctctggatt a

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