Construct: ORF ccsbBroad304_15505
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005249.1_s304c1, BRDN0000399073
- Derived from:
- ccsbBroadEn_15505
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NDUFV1 (4723)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_15505
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4723 | NDUFV1 | NADH:ubiquinone oxidoreduct... | NM_001166102.2 | 100% | 100% | |
| 2 | human | 4723 | NDUFV1 | NADH:ubiquinone oxidoreduct... | NM_007103.4 | 98% | 98% | 45_71del |
| 3 | mouse | 17995 | Ndufv1 | NADH dehydrogenase (ubiquin... | NM_133666.3 | 87.9% | 94.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1431
- ORF length:
- 1365
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgct ggcaacacgg cggctgctcg gctggtcgct tcccgcgcgg acagcaccca 121 agaaaacctc atttggctcg ctgaaggatg aagaccggat tttcaccaac ctgtacggcc 181 gccatgactg gaggctgaaa ggttccctga gtcgaggtga ctggtacaag acaaaggaga 241 tcctgctgaa ggggcccgac tggatcctgg gcgagatcaa gacatcgggt ttgaggggcc 301 gtggaggcgc tggcttcccc actggcctca agtggagctt catgaataag ccctcagatg 361 gcaggcccaa gtatctggtg gtgaacgcag acgaggggga gccgggcacc tgcaaggacc 421 gggagatctt acgccatgat cctcacaagc tgctggaagg ctgcctggtg gggggccggg 481 ccatgggcgc ccgcgctgcc tatatctaca tccgagggga attctacaat gaggcctcca 541 atctgcaggt ggccatccga gaggcctatg aggcaggtct gattggcaag aatgcttgtg 601 gctctggcta tgattttgac gtgtttgtgg tgcgcggggc tggggcctac atctgtggag 661 aggagacagc gctcatcgag tccattgagg gcaagcaggg caagccccgc ctgaagcccc 721 ccttccccgc agacgtggga gtgtttggct gccccacaac tgtggccaac gtggagacag 781 tggcagtgtc ccccacaatc tgccgccgtg gaggtacctg gtttgctggc tttggcagag 841 aacgcaactc aggcaccaaa ctattcaaca tctctggcca tgtcaaccac ccttgcactg 901 tggaggagga gatgtctgtg cccttgaaag aactgattga gaagcatgct gggggtgtca 961 cgggcggctg ggacaacctc cttgctgtga tccctggcgg ctcgtctacc ccactgatcc 1021 ccaagtctgt gtgtgagacg gtgctgatgg acttcgatgc gctggtgcag gcacagacag 1081 gcctgggcac agctgcggtg atcgtcatgg accgctcgac ggacatcgtg aaagccatcg 1141 cccgcctcat tgagttctat aagcacgaga gctgtggcca gtgtacccca tgccgtgagg 1201 gtgtggactg gatgaacaag gtgatggcac gtttcgtgag gggggatgcc cggccggccg 1261 agatcgactc cctgtgggag atcagcaagc agatagaagg ccatacgatt tgtgctctgg 1321 gtgacggggc cgcctggcct gtgcagggtc tgatccgcca ctttcggccg gagctcgagg 1381 agcggatgca gcggtttgcc cagcagcatc aggcccggca ggctgcctct tnnccaactt 1441 tcttgtacaa agtggttggt aagcctatcc ctaaccctct cctcggtctc gattctacgt 1501 agtaatgagc tagcgctaac cggtggcgcg ttaagtcgac aatcaacctc tggatta