Construct: ORF ccsbBroad304_15542
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017766.1_s304c1, BRDN0000402913
- Derived from:
- ccsbBroadEn_15542
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PSMA3 (5684)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_15542
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5684 | PSMA3 | proteasome 20S subunit alpha 3 | NM_152132.2 | 100% | 100% | |
| 2 | human | 5684 | PSMA3 | proteasome 20S subunit alpha 3 | NM_002788.4 | 97.2% | 97.2% | 402_422del |
| 3 | human | 5684 | PSMA3 | proteasome 20S subunit alpha 3 | NR_038123.1 | 72.7% | (many diffs) | |
| 4 | mouse | 19167 | Psma3 | proteasome (prosome, macrop... | NM_011184.5 | 89.4% | 96% | (many diffs) |
| 5 | mouse | 19167 | Psma3 | proteasome (prosome, macrop... | XM_017314996.1 | 89.4% | 96% | (many diffs) |
| 6 | mouse | 19167 | Psma3 | proteasome (prosome, macrop... | NM_001310595.1 | 62% | 66.6% | (many diffs) |
| 7 | mouse | 19167 | Psma3 | proteasome (prosome, macrop... | NM_001310596.1 | 62% | 66.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 810
- ORF length:
- 744
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgag ctcaatcggc actgggtatg acctgtcagc ctctacattc tctcctgacg 121 gaagagtttt tcaagttgaa tatgctatga aggctgtgga aaatagtagt acagctattg 181 gaatcagatg caaagatggt gttgtctttg gggtagaaaa attagtcctt tctaaacttt 241 atgaagaagg ttccaacaaa agacttttta atgttgatcg gcatgttgga atggcagtag 301 caggtttgtt ggcagatgct cgttctttag cagacatagc aagagaagaa gcttccaact 361 tcagatctaa ctttggctac aacattccac taaaacatct tgcagacaga gtggccatgt 421 atgtgcatgc atatacactc tacagtgctg ttagaccttt tggctgcagt gtgaatgacg 481 gtgcgcaact ctacatgatt gacccatcag gtgtttcata cggttattgg ggctgtgcca 541 tcggcaaagc caggcaagct gcaaagacgg aaatagagaa gcttcagatg aaagaaatga 601 cctgccgtga tatcgttaaa gaagttgcaa aaataattta catagtacat gacgaagtta 661 aggataaagc ttttgaacta gaactcagct gggttggtga attaactaat ggaagacatg 721 aaattgttcc aaaagatata agagaagaag cagagaaata tgctaaggaa tctctgaagg 781 aagaagatga atcagatgat gataatatgt nnccaacttt cttgtacaaa gtggttggta 841 agcctatccc taaccctctc ctcggtctcg attctacgta gtaatgagct agcgctaacc 901 ggtggcgcgt taagtcgaca atcaacctct ggatta