Construct: ORF ccsbBroad304_15577
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017608.1_s304c1, BRDN0000411091
- Derived from:
- ccsbBroadEn_15577
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RPS2 (6187)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_15577
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6187 | RPS2 | ribosomal protein S2 | NM_002952.4 | 99.8% | 99.6% | 605C>T |
| 2 | human | 256355 | RPS2P32 | ribosomal protein S2 pseudo... | NR_026676.1 | 76.6% | (many diffs) | |
| 3 | mouse | 16898 | Rps2 | ribosomal protein S2 | NM_008503.5 | 87.2% | 98.2% | (many diffs) |
| 4 | mouse | 677044 | Gm10653 | predicted gene 10653; ribso... | NR_003965.2 | 56.6% | (many diffs) | |
| 5 | mouse | 108167548 | Gm45855 | predicted gene 45855; 40S r... | XR_001778644.1 | 49% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 945
- ORF length:
- 879
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc ggatgacgcc ggtgcagcgg gggggcccgg gggccctggt ggccctggga 121 tggggaaccg cggtggcttc cgcggaggtt tcggcagtgg catccggggc cggggtcgcg 181 gccgtggacg gggccggggc cgaggccgcg gagctcgcgg aggcaaggcc gaggataagg 241 agtggatgcc cgtcaccaag ttgggccgct tggtcaagga catgaagatc aagtccctgg 301 aggagatcta tctcttctcc ctgcctatta aggaatcaga gatcattgat ttcttcctgg 361 gggcctctct caaggatgag gttttgaaga ttatgccagt gcagaagcag acccgtgccg 421 gccagcgcac caggttcaag gcatttgttg ctatcgggga ctacaatggc cacgtcggtc 481 tgggtgttaa gtgctccaag gaggtggcca ccgccatccg tggggccatc atcctggcca 541 agctctccat cgtccccgtg cgcagaggct actgggggaa caagatcggc aagccccaca 601 ctgtcccttg caaggtgaca ggccgctgcg gctctgtgct ggtacgcctc atccctgcac 661 ccaggggcat tggcatcgtc tccgcacctg tgcctaagaa gctgctcatg atggctggta 721 tcgatgactg ctacacctca gcccggggct gcactgccac cctgggcaac ttcgccaagg 781 ccacctttga tgccatttct aagacctaca gctacctgac ccccgacctc tggaaggaga 841 ctgtattcac caagtctccc tatcaggagt tcactgacca cctcgtcaag acccacacca 901 gagtctccgt gcagcggact caggctccag ctgtggctac aacatnncca actttcttgt 961 acaaagtggt tggtaagcct atccctaacc ctctcctcgg tctcgattct acgtagtaat 1021 gagctagcgc taaccggtgg cgcgttaagt cgacaatcaa cctctggatt a