Construct: ORF ccsbBroad304_15590
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012850.1_s304c1, BRDN0000404543
- Derived from:
- ccsbBroadEn_15590
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ST8SIA1 (6489)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_15590
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6489 | ST8SIA1 | ST8 alpha-N-acetyl-neuramin... | NM_003034.3 | 22.8% | 22.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 315
- ORF length:
- 249
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgag cccctgcggg cgggcccggc gacaaacgtc cagaggggcc atggctgtac 121 tggcgtggaa gttcccgcgg acccggctgc ccatgggagc cagtgccctc tgtgtcgtgg 181 tcctctgttg gctctacatc ttccccgtct accggctgcc caacgagaaa gagatcgtgc 241 agggggtgct gcaacagggc acggcgtgga ggaggaacca gaccgcggcc agagcgttca 301 gctgtggcat tgcatnncca actttcttgt acaaagtggt tggtaagcct atccctaacc 361 ctctcctcgg tctcgattct acgtagtaat gagctagcgc taaccggtgg cgcgttaagt 421 cgacaatcaa cctctggatt a