Construct: ORF ccsbBroad304_15669
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010547.1_s304c1, BRDN0000408830
- Derived from:
- ccsbBroadEn_15669
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MED26 (9441)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_15669
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9441 | MED26 | mediator complex subunit 26 | NM_004831.4 | 99.9% | 99.8% | 1106G>T |
| 2 | mouse | 70625 | Med26 | mediator complex subunit 26 | NM_027485.4 | 82.3% | 84.5% | (many diffs) |
| 3 | mouse | 70625 | Med26 | mediator complex subunit 26 | XM_006509739.3 | 77.9% | 80.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1869
- ORF length:
- 1800
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gacagcggct ccggcgtctc cgcagcagat cagggaccgg ctgctgcagg 121 ccatcgaccc ccagagcaac atccggaaca tggtggcggt gctggaagtc atctccagcc 181 tggagaaata ccctattacc aaagaggcac ttgaggaaac acgacttggg aagctcatca 241 acgacgtccg caagaaaacc aagaacgagg agctcgccaa gcgggccaag aagctgctgc 301 ggagctggca gaagctcatc gagccggcac accagcatga ggcggcgctg cgggggctgg 361 cgggggccac cggctctgcc aacgggggcg cacacaactg ccggccggag gtgggggcgg 421 ctggcccacc caggagcatc catgacctga agagccgcaa tgacctccag aggctgcccg 481 ggcagcggct ggacaggctg ggcagccgca agcgccgggg tgaccagcgt gacctcggcc 541 acccagggcc gccacccaag gtctccaaag ctagccacga ccccctggtc cccaactcat 601 cccccctccc caccaacggg atcagtggga gtccagagag cttcgccagc tccctggatg 661 gcagtgggca tgcaggccca gagggcagcc gcctggagcg tgacgagaat gacaagcaca 721 gtggcaagat ccccgtcaac gccgtgcgac cgcacaccag ctccccgggc ctgggcaagc 781 cccctggacc ctgcttgcag ccaaaggctt cggtgctgca gcagctggac agggtggacg 841 agactccggg gcctccccat cccaagggac cccctcgctg ctctttcagt cctcggaact 901 cacggcatga gggctccttt gcccggcagc agagcttgta tgcacccaag ggctccgtgc 961 ccagcccctc accgcggccc caggcactcg atgccacaca ggtgccgtca ccgcttccac 1021 tggcacagcc gtccacaccc cccgtacggc ggctcgagct gctgcccagt gcggaaagcc 1081 cagtgtgctg gcttgagcag cctgagagcc accagcggct ggcggggccg ggctgcaagg 1141 cagggctgtc cccagccgag cccctcctgt ccctggcagg cttttcccca gactcctcca 1201 aggcggacag tgatgctgcc tcctcagggg gctcggacag taaaaagaag aagaggtacc 1261 gacctcgaga ctatacggtt aacttggacg ggcaggtggc tgaggcgggc gtcaagcctg 1321 tccggttaaa agagcggaag ctcacctttg accccatgac gagacagatc aaacctctga 1381 cccagaaaga gccagtgcgg gcagacagcc ctgtgcacat ggagcagcag tccaggacag 1441 agctggacaa gcaggaggcc aaggccagcc tccagagccc cttcgaacag acgaactgga 1501 aggagctgtc acgcaacgag atcatccagt cctacctgag ccggcagagc agcctgctct 1561 catcatcggg cgcgcagacc ccaggggctc accacttcat gtctgagtac ctgaagcagg 1621 aggagagcac ccggcaaggg gccaggcagc tgcatgtgct ggtgcctcaa agcccgccca 1681 cggacctccc tggtctgacc cgggaggtca cacaggacga tctcgacaga atccaggcca 1741 gccagtggcc gggggtgaac gggtgtcagg acacacaggg taactggtat gactggacgc 1801 agtgcatatc gctcgatccg cacggcgacg acgggcgctt gaacattctg ccttatgtct 1861 gcttggactn nccaactttc ttgtacaaag tggttggtaa gcctatccct aaccctctcc 1921 tcggtctcga ttctacgtag taatgagcta gcgctaaccg gtggcgcgtt aagtcgacaa 1981 tcaacctctg gatta