Construct: ORF ccsbBroad304_15719

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF013415.1_s304c1, BRDN0000411785
Derived from:
ccsbBroadEn_15719
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
CCT2 (10576)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_15719
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 10576 CCT2 chaperonin containing TCP1 ... NM_006431.3 99.9% 100% 783A>G
2 human 10576 CCT2 chaperonin containing TCP1 ... NM_001198842.2 91.1% 91.2% 0_1ins141;642A>G
3 mouse 12461 Cct2 chaperonin containing Tcp1,... NM_007636.2 89% 97.5% (many diffs)
4 mouse 12461 Cct2 chaperonin containing Tcp1,... XM_006513161.2 89% 97.5% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1674
ORF length:
1605
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat ggcgtccctt tcccttgcac ctgttaacat ctttaaggca ggagctgatg
121aagagagagc agagacagct cgtctgactt cttttattgg tgccatcgcc attggagact
181tggtaaagag caccttggga cccaaaggca tggacaaaat tcttctaagc agtggacgag
241atgcctctct tatggtaacc aatgatggtg ccactattct aaaaaacatt ggtgttgaca
301atccagcagc taaagtttta gttgatatgt caagggttca agatgatgaa gttggtgatg
361gcactacctc tgttaccgtt ttagcagcag aattattaag ggaagcagaa tctttaattg
421caaaaaagat tcatccacag accatcatag cgggttggag agaagccacg aaggctgcaa
481gagaggcgct gttgagttct gcagttgatc atggttccga tgaagttaaa ttccgtcaag
541atttaatgaa tattgcgggc acaacattat cctcaaaact tcttactcat cacaaagacc
601actttacaaa gttagctgta gaagcagttc tcagactgaa aggctctggc aacctggagg
661caattcatat tatcaagaag ctaggaggaa gtttggcaga ttcctattta gatgaaggct
721tcctgttgga taaaaaaatt ggagtaaatc aaccaaaacg aattgaaaat gctaaaattc
781ttattgcaaa tactggtatg gatacagaca aaataaagat atttggttcc cgggtaagag
841ttgactctac ggcaaaggtt gcagaaatag aacatgcgga aaaggaaaaa atgaaggaga
901aagttgaacg tattcttaag catggaataa attgctttat taacaggcaa ttaatttata
961attatcctga acagctcttt ggtgctgctg gtgtcatggc tattgagcat gcagattttg
1021caggtgtgga acgcctagct cttgtcacag gtggtgaaat tgcctctacc tttgatcacc
1081cagaactggt gaagcttgga agttgcaaac ttatcgagga agtcatgatt ggagaagaca
1141aactcattca cttttctggg gttgcccttg gtgaggcttg taccattgtt ttgcgtggtg
1201ccactcaaca aattttagat gaagcagaaa gatcattgca tgatgctctt tgtgttcttg
1261cgcaaactgt aaaggactct agaacagttt atggaggagg ctgttctgag atgttgatgg
1321ctcatgctgt gacacagctt gccaatagaa caccaggcaa agaagctgtt gcaatggagt
1381cttatgctaa agcactgaga atgttgccaa ccatcatagc tgacaatgca ggctatgaca
1441gtgcagacct ggtggcacag ctcagggctg ctcacagtga aggcaatacc actgctggat
1501tggatatgag ggaaggcacc attggagata tggctatcct gggtataaca gaaagttttc
1561aagtgaagcg acaggttctt ctgagtgcag ctgaagcagc agaggtgatt ctgcgtgtgg
1621acaacatcat caaagcggca cccaggaaac gtgtccctga tcaccacccc tgttnnccaa
1681ctttcttgta caaagtggtt ggtaagccta tccctaaccc tctcctcggt ctcgattcta
1741cgtagtaatg agctagcgct aaccggtggc gcgttaagtc gacaatcaac ctctggatta

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