Construct: ORF ccsbBroad304_15761
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007420.1_s304c1, BRDN0000403938
- Derived from:
- ccsbBroadEn_15761
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CBX6 (23466)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_15761
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23466 | CBX6 | chromobox 6 | NM_014292.5 | 100% | 100% | |
| 2 | human | 23466 | CBX6 | chromobox 6 | NM_001303494.2 | 95.6% | 95.6% | 243_244ins54 |
| 3 | mouse | 494448 | Cbx6 | chromobox 6 | NM_028763.3 | 85.7% | 88.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1302
- ORF length:
- 1236
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgga gctgtctgca gtgggcgagc gggtcttcgc ggccgaatcc atcatcaaac 121 ggcggatccg aaagggacgc atcgagtacc tggtgaaatg gaaggggtgg gcgatcaagt 181 acagcacttg ggagcccgag gagaacatcc tggactcgcg gctcattgca gccttcgaac 241 aaaaggagag ggagcgtgag ctgtatgggc ccaagaagag gggacccaaa cccaaaactt 301 tcctcctgaa ggcgcgggcc caggccgagg ccctccgcat cagtgatgtg catttctctg 361 tcaagccgag cgccagtgcc tcctcgccca agctgcactc cagcgcagcc gtgcaccggc 421 tcaagaagga catccgccgc tgccaccgta tgtcccgccg tcccctgccc cgcccggacc 481 cgcagggggg cagccccgga ctgcgcccgc ccatttcgcc cttctcggag acggtgcgca 541 tcatcaaccg caaggtgaag ccgcgggagc ccaagcggaa ccgcatcatc ctgaacctga 601 aggtgatcga caagggcgct ggcggcgggg gcgccgggca gggggccggg gcgctggccc 661 gccccaaagt cccctcgcgg aaccgcgtta taggcaagag caagaagttc agcgagagcg 721 tcctgcgtac acagatccgc cacatgaagt tcggcgcctt tgcgctgtac aagcctccgc 781 ccgcccccct ggtagccccg tcccccggca aggctgaggc ctcagccccg ggccctgggc 841 tacttctggc cgcccccgcc gccccctacg acgcccgcag ctctggctcc tccggctgcc 901 cctcgcctac accacagtcc tctgaccccg acgacacgcc ccccaagctc ctccccgaga 961 ccgtgagccc atccgccccc agctggcgcg agccggaggt gctcgacctg tccctccctc 1021 ccgagtcggc agccaccagc aagcgggcac cgcctgaggt cacagctgct gccggcccgg 1081 cacctcccac ggcccctgag cccgccggtg cctcctccga gcccgaggct ggggactggc 1141 gccccgagat gtcaccctgc tccaatgtgg tcgtcaccga tgtcaccagc aacctcctga 1201 cggtcacaat caaggaattc tgcaaccctg aggatttcga gaaggtggct gctggggtag 1261 caggcgccgc tgggggcggt ggcagcattg gggcgagcaa gtnnccaact ttcttgtaca 1321 aagtggttgg taagcctatc cctaaccctc tcctcggtct cgattctacg tagtaatgag 1381 ctagcgctaa ccggtggcgc gttaagtcga caatcaacct ctggatta