Construct: ORF ccsbBroad304_15912

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF009506.1_s304c1, BRDN0000404733
Derived from:
ccsbBroadEn_15912
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
ARFGAP1 (55738)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_15912
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 55738 ARFGAP1 ADP ribosylation factor GTP... NM_001281482.2 100% 100%
2 human 55738 ARFGAP1 ADP ribosylation factor GTP... NM_018209.4 66.5% 66.8% 834_835ins239;971_1218del
3 human 55738 ARFGAP1 ADP ribosylation factor GTP... XM_006723823.3 66.1% 66.5% 832_833ins245;965_1212del
4 human 55738 ARFGAP1 ADP ribosylation factor GTP... XM_011528901.2 65.2% 65.3% 717_746del;864_865ins239;1001_1248del
5 human 55738 ARFGAP1 ADP ribosylation factor GTP... XM_017027950.1 65.2% 65.3% 717_746del;864_865ins239;1001_1248del
6 human 55738 ARFGAP1 ADP ribosylation factor GTP... NM_175609.3 64.8% 65.1% 717_746del;862_863ins245;995_1242del
7 human 55738 ARFGAP1 ADP ribosylation factor GTP... NM_001281483.1 53.3% 52.7% (many diffs)
8 human 55738 ARFGAP1 ADP ribosylation factor GTP... NM_001281484.1 43.3% 35.9% (many diffs)
9 human 55738 ARFGAP1 ADP ribosylation factor GTP... XR_001754329.2 29% (many diffs)
10 human 55738 ARFGAP1 ADP ribosylation factor GTP... XR_936574.2 28.1% (many diffs)
11 human 55738 ARFGAP1 ADP ribosylation factor GTP... XR_936576.2 26.2% (many diffs)
12 human 55738 ARFGAP1 ADP ribosylation factor GTP... XR_936575.2 26% (many diffs)
13 human 55738 ARFGAP1 ADP ribosylation factor GTP... XR_001754330.2 25.8% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1275
ORF length:
1209
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggc cagcccaaga accaggaagg ttcttaaaga agtcagggtg caggatgaga
121acaacgtttg ttttgagtgt ggcgcgttca atcctcagtg ggtcagtgtg acctacggca
181tctggatctg cctggagtgc tcggggagac accgcgggct tggggttcac ctcagctttg
241tgcgctctgt tactatggac aagtggaagg acattgagct tgagaagatg aaagctggtg
301ggaatgctaa gttccgagag ttcctggagt ctcaggagga ttacgatcct tgctggtcct
361tgcaggagaa gtacaacagc agagccgcgg ccctctttag ggataaggtg gtcgctctgg
421ccgaaggcag agagtggtct ctggagtcat cacctgccca gaactggacc ccacctcagc
481ccaggacgct gccgtccatg gtgcaccgag tctctggcca gccgcagagt gtgaccgcct
541cctcggacaa ggcttttgaa gactggctga atgatgacct cggctcctat caaggggccc
601aggggaatcg ctacgtgggg tttgggaaca cgccaccgcc tcagaagaaa gaagatgact
661tcctcaacaa cgccatgtcc tccctgtact cgggctggag cagcttcacc actggagcca
721gccggtttgc ctcggcagcc aaggagggcg ctacaaagtt tggatcccaa gcgagtcaga
781aggcgtccga gctgggccac agcctgaacg agaacgtcct caagcctgcg caggagaagg
841tgaaggaggg aaagattttt gatgatgtct ccagtggggt ctctcagttg gcgtccaagt
901gccagcgccg tctttgctgc catcagtccc actgctctgc gggccatttg gggcgtgcat
961tttgtcctgt ttcctggcat gaggcgctct gcggacagac ggggagggaa gagcaggcct
1021cgctcctccc ccccaagcat gtggtgggag ctcttgaggt ctgtgcacga ggctgtcctc
1081gctgccatgt cccgcacaca cctggcaccg ctgcagagtg gccggggcgt ctgtgtctgt
1141ccagggagtc ggtagtaagg gatggcggga cgtcaccacc tttttttcgg ggaaagcaga
1201gggccccttg gacagcccct cggagggcca cagttatcag aacagcggtc tggaccactt
1261ccaaaacagc aacatnncca actttcttgt acaaagtggt tggtaagcct atccctaacc
1321ctctcctcgg tctcgattct acgtagtaat gagctagcgc taaccggtgg cgcgttaagt
1381cgacaatcaa cctctggatt a

Download FASTA (ORF) (Full)