Construct: ORF ccsbBroad304_15972
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004389.1_s304c1, BRDN0000399212
- Derived from:
- ccsbBroadEn_15972
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RBM15 (64783)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_15972
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 64783 | RBM15 | RNA binding motif protein 15 | NM_022768.4 | 95.4% | 95.4% | 1_132del;1542A>T |
| 2 | human | 64783 | RBM15 | RNA binding motif protein 15 | NM_001201545.1 | 93.3% | 93.3% | (many diffs) |
| 3 | mouse | 229700 | Rbm15 | RNA binding motif protein 15 | NM_001045807.1 | 84.3% | 88.9% | (many diffs) |
| 4 | mouse | 229700 | Rbm15 | RNA binding motif protein 15 | XR_001783686.1 | 12.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2865
- ORF length:
- 2799
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgaa gggaaaagag cgctcgccag tgaaggccaa acgctcccgt ggtggtgagg 121 actcgacttc ccgcggtgag cggagcaaga agttaggggg ctctggtggc agcaatggga 181 gcagcagcgg aaagaccgat agcggcggtg ggtcgcggcg gagtctccac ctggacaagt 241 ccagcagtcg aggtggcagc cgcgagtatg ataccggtgg gggcagctcc agtagccgct 301 tgcatagtta tagctccccg agcaccaaaa attcttcggg cgggggcgag tcgcgcagca 361 gctcccgggg tggaggcggg gagtcacgtt cctctggggc cgcctcctca gctcccggcg 421 gcggggacgg cgcggaatac aagactctga agataagcga gttggggtcc cagcttagtg 481 acgaagcggt ggaggacggc ctgtttcatg agttcaaacg cttcggtgat gtaagtgtga 541 aaatcagtca tctgtcgggt tctggcagcg gggatgagcg ggtagccttt gtgaacttcc 601 ggcggccaga ggacgcgcgg gcggccaagc atgccagagg ccgcctggtg ctctatgacc 661 ggcctctgaa gatagaagct gtgtatgtga gccggcgccg cagccgctcc cctttagaca 721 aagatactta tcctccatca gccagtgtgg tcggggcctc tgtaggtggt caccggcacc 781 cccctggagg tggtggaggc cagagatcac tttcccctgg tggcgctgct ttgggataca 841 gagactaccg gctgcagcag ttggctcttg gccgcctgcc ccctccacct ccgccaccat 901 tgcctcgaga cctggagaga gaaagagact acccgttcta tgagagagtg cgccctgcat 961 acagtcttga gccaagggtg ggagctggag caggtgctgc tcctttcaga gaagtggatg 1021 agatttcacc cgaggatgat cagcgagcta accggacgct cttcttgggc aacctagaca 1081 tcactgtaac ggagagtgat ttaagaaggg cgtttgatcg ctttggagtc atcacagaag 1141 tagatatcaa gaggccttct cgcggccaga ctagtactta cggctttctc aaatttgaga 1201 acttagatat gtctcaccgg gccaaattag caatgtctgg caaaattata attcggaatc 1261 ctatcaaaat tggttatggt aaagctacac ccaccacccg cctctgggtg ggaggcctgg 1321 gaccttgggt tcctcttgct gccctggcac gagaatttga tcgatttggc accatacgca 1381 ccatagacta ccgaaaaggt gatagttggg catatatcca gtatgaaagc ctggatgcag 1441 cgcatgctgc ctggacccat atgcggggct tccctcttgg tggcccagat cgacgcctta 1501 gagtagactt tgccgacacc gaacatcgtt accagcagca gtatctgcag cctctgccct 1561 tgactcatta tgagctggtg acagatgctt ttggacatcg ggcaccagac cctttgaggg 1621 gtgctcggga taggacacca cccttactat acagagatcg tgatagggac ctttatcctg 1681 actctgattg ggtgccaccc ccacccccag tccgagaacg cagcactcgg actgcagcta 1741 cttctgtgcc tgcttacgag ccactggata gcctagatcg caggcgggat ggttggtcct 1801 tggaccggga cagaggtgat cgagatctgc ccagcagcag agaccagcct aggaagcgaa 1861 ggctgcctga ggagagtgga ggacgtcatc tggataggtc tcctgagagt gaccgcccac 1921 gaaaacgtca ctgcgctcct tctcctgacc gcagtccaga attgagcagt agccgggatc 1981 gttacaacag cgacaatgat cgatcttccc gtcttctctt ggaaaggccc tctccaatca 2041 gagacagacg aggtagtttg gagaagagcc agggtgacaa gcgagaccgt aaaaactctg 2101 catcagctga acgagatagg aagcaccgga caactgctcc cactgaggga aaaagccctc 2161 tgaaaaaaga agaccgctct gatgggagtg cacctagcac cagcactgct tcctccaagc 2221 tgaagtcccc gtcccagaaa caggatgggg ggacagcccc tgtggcatca gcctctccca 2281 aactctgttt ggcctggcag ggcatgcttc tactgaagaa cagcaacttt ccttccaaca 2341 tgcatctgtt gcagggtgac ctccaagtgg ctagtagtct tcttgtggag ggttcaactg 2401 gaggcaaagt ggcccagctc aagatcactc agcgtctccg tttggaccag cccaagttgg 2461 atgaagtaac tcgacgcatc aaagtagcag ggcccaatgg ttatgccatt cttttggctg 2521 tgcctggaag ttctgacagc cggtcctcct cttcctcagc tgcatcagac actgccactt 2581 ctactcagag gccacttagg aaccttgtgt cctatttaaa gcaaaagcag gcagccgggg 2641 tgatcagcct ccctgtgggg ggcaacaaag acaaggaaaa caccggggtc cttcatgcct 2701 tcccaccttg tgagttctcc cagcagttcc tggattcccc tgccaaggca ctggccaaat 2761 ctgaagaaga ttacctggtc atgatcattg tccgtgggtt tggttttcag ataggagtta 2821 ggtatgagaa caagaagaga gaaaacttgg cgctgaccct gttatnncca actttcttgt 2881 acaaagtggt tggtaagcct atccctaacc ctctcctcgg tctcgattct acgtagtaat 2941 gagctagcgc taaccggtgg cgcgttaagt cgacaatcaa cctctggatt a