Construct: ORF ccsbBroadEn_15972

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF004389.1_s300c1, BRDN0000385468
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
RBM15 (64783)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 64783 RBM15 RNA binding motif protein 15 NM_022768.4 95.4% 95.4% 1_132del;1542A>T
2 human 64783 RBM15 RNA binding motif protein 15 NM_001201545.1 93.3% 93.3% (many diffs)
3 mouse 229700 Rbm15 RNA binding motif protein 15 NM_001045807.1 84.3% 88.9% (many diffs)
4 mouse 229700 Rbm15 RNA binding motif protein 15 XR_001783686.1 12.5% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2865
ORF length:
2799
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaactttg tacaaaaaag
61ttggcatgaa gggaaaagag cgctcgccag tgaaggccaa acgctcccgt ggtggtgagg
121actcgacttc ccgcggtgag cggagcaaga agttaggggg ctctggtggc agcaatggga
181gcagcagcgg aaagaccgat agcggcggtg ggtcgcggcg gagtctccac ctggacaagt
241ccagcagtcg aggtggcagc cgcgagtatg ataccggtgg gggcagctcc agtagccgct
301tgcatagtta tagctccccg agcaccaaaa attcttcggg cgggggcgag tcgcgcagca
361gctcccgggg tggaggcggg gagtcacgtt cctctggggc cgcctcctca gctcccggcg
421gcggggacgg cgcggaatac aagactctga agataagcga gttggggtcc cagcttagtg
481acgaagcggt ggaggacggc ctgtttcatg agttcaaacg cttcggtgat gtaagtgtga
541aaatcagtca tctgtcgggt tctggcagcg gggatgagcg ggtagccttt gtgaacttcc
601ggcggccaga ggacgcgcgg gcggccaagc atgccagagg ccgcctggtg ctctatgacc
661ggcctctgaa gatagaagct gtgtatgtga gccggcgccg cagccgctcc cctttagaca
721aagatactta tcctccatca gccagtgtgg tcggggcctc tgtaggtggt caccggcacc
781cccctggagg tggtggaggc cagagatcac tttcccctgg tggcgctgct ttgggataca
841gagactaccg gctgcagcag ttggctcttg gccgcctgcc ccctccacct ccgccaccat
901tgcctcgaga cctggagaga gaaagagact acccgttcta tgagagagtg cgccctgcat
961acagtcttga gccaagggtg ggagctggag caggtgctgc tcctttcaga gaagtggatg
1021agatttcacc cgaggatgat cagcgagcta accggacgct cttcttgggc aacctagaca
1081tcactgtaac ggagagtgat ttaagaaggg cgtttgatcg ctttggagtc atcacagaag
1141tagatatcaa gaggccttct cgcggccaga ctagtactta cggctttctc aaatttgaga
1201acttagatat gtctcaccgg gccaaattag caatgtctgg caaaattata attcggaatc
1261ctatcaaaat tggttatggt aaagctacac ccaccacccg cctctgggtg ggaggcctgg
1321gaccttgggt tcctcttgct gccctggcac gagaatttga tcgatttggc accatacgca
1381ccatagacta ccgaaaaggt gatagttggg catatatcca gtatgaaagc ctggatgcag
1441cgcatgctgc ctggacccat atgcggggct tccctcttgg tggcccagat cgacgcctta
1501gagtagactt tgccgacacc gaacatcgtt accagcagca gtatctgcag cctctgccct
1561tgactcatta tgagctggtg acagatgctt ttggacatcg ggcaccagac cctttgaggg
1621gtgctcggga taggacacca cccttactat acagagatcg tgatagggac ctttatcctg
1681actctgattg ggtgccaccc ccacccccag tccgagaacg cagcactcgg actgcagcta
1741cttctgtgcc tgcttacgag ccactggata gcctagatcg caggcgggat ggttggtcct
1801tggaccggga cagaggtgat cgagatctgc ccagcagcag agaccagcct aggaagcgaa
1861ggctgcctga ggagagtgga ggacgtcatc tggataggtc tcctgagagt gaccgcccac
1921gaaaacgtca ctgcgctcct tctcctgacc gcagtccaga attgagcagt agccgggatc
1981gttacaacag cgacaatgat cgatcttccc gtcttctctt ggaaaggccc tctccaatca
2041gagacagacg aggtagtttg gagaagagcc agggtgacaa gcgagaccgt aaaaactctg
2101catcagctga acgagatagg aagcaccgga caactgctcc cactgaggga aaaagccctc
2161tgaaaaaaga agaccgctct gatgggagtg cacctagcac cagcactgct tcctccaagc
2221tgaagtcccc gtcccagaaa caggatgggg ggacagcccc tgtggcatca gcctctccca
2281aactctgttt ggcctggcag ggcatgcttc tactgaagaa cagcaacttt ccttccaaca
2341tgcatctgtt gcagggtgac ctccaagtgg ctagtagtct tcttgtggag ggttcaactg
2401gaggcaaagt ggcccagctc aagatcactc agcgtctccg tttggaccag cccaagttgg
2461atgaagtaac tcgacgcatc aaagtagcag ggcccaatgg ttatgccatt cttttggctg
2521tgcctggaag ttctgacagc cggtcctcct cttcctcagc tgcatcagac actgccactt
2581ctactcagag gccacttagg aaccttgtgt cctatttaaa gcaaaagcag gcagccgggg
2641tgatcagcct ccctgtgggg ggcaacaaag acaaggaaaa caccggggtc cttcatgcct
2701tcccaccttg tgagttctcc cagcagttcc tggattcccc tgccaaggca ctggccaaat
2761ctgaagaaga ttacctggtc atgatcattg tccgtgggtt tggttttcag ataggagtta
2821ggtatgagaa caagaagaga gaaaacttgg cgctgaccct gttatnncca actttcttgt
2881acaaagttgg cattataaga aagcattgct tatcaatttg ttgcaacgaa c

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