Construct: ORF ccsbBroad304_15973
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013581.1_s304c1, BRDN0000403464
- Derived from:
- ccsbBroadEn_15973
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DEPTOR (64798)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_15973
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 64798 | DEPTOR | DEP domain containing MTOR ... | NM_022783.4 | 99.8% | 99.7% | 135C>T;611A>G |
| 2 | human | 64798 | DEPTOR | DEP domain containing MTOR ... | NM_001283012.1 | 75.2% | 75% | 122_123ins303;308A>G |
| 3 | mouse | 97998 | Deptor | DEP domain containing MTOR-... | NM_145470.3 | 89.9% | 95.8% | (many diffs) |
| 4 | mouse | 97998 | Deptor | DEP domain containing MTOR-... | NM_001037937.3 | 84% | 87% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1293
- ORF length:
- 1227
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgga ggagggcggc agcactggca gtgctggcag tgacagcagc accagcggga 121 gtggcggggc gcagcaaagg gagctggagc gcatggctga ggtcttggtc accggggaac 181 agctacggct caggctgcat gaagaaaagg ttattaaaga tagacgtcat catctcaaga 241 cctacccaaa ctgttttgtc gcaaaagaac tgattgactg gctgattgaa cacaaagagg 301 cttctgacag agagacggca attaaactca tgcagaaatt agcagaccgg ggcattattc 361 accatgtgtg tgatgagcat aaggaattca aggatgtcaa actcttctac cgctttagaa 421 aggatgacgg caccttccca ttggataatg aagtgaaggc ctttatgaga ggacagaggc 481 tatatgaaaa gctgatgagc cctgaaaaca cactcctgca gcccagggag gaggaagggg 541 tcaagtatga gcgcaccttc atggcatctg aattcctgga ctggctggtt caggaaggtg 601 aggccaccac gaggaaagag gcagagcagc tttgccaccg gcttatggag catggcatca 661 tccagcatgt gtccagcaag cacccatttg tggacagcaa tcttctctac cagttcagaa 721 tgaacttccg gcggaggcga agactgatgg agctgctcaa tgaaaagtcc ccctcctccc 781 aggaaactca tgacagtccc ttctgcctga ggaagcagag ccatgacaat cggaaatcta 841 ccagctttat gtcagtgagc cccagcaagg agatcaagat cgtgtctgca gtgaggagaa 901 gcagcatgag cagctgtggc agcagcggct acttcagcag cagccccacc ctcagcagca 961 gcccccctgt gctctgcaac cccaagtccg tgctgaagag acctgtcacc tctgaggaac 1021 tccttactcc cggggctccg tatgcaagga agacattcac gattgttggt gacgcggttg 1081 gctggggttt tgtggtgcga ggaagtaagc catgccacat ccaggctgta gaccccagtg 1141 gccctgcagc cgcagcagga atgaaggtct gtcagtttgt cgtctctgtc aacgggctca 1201 atgtcctgca tgtagactac cggaccgtga gcaatctgat tctgacgggc ccacggacga 1261 ttgtcatgga agtcatggag gagttagagt gctnnccaac tttcttgtac aaagtggttg 1321 gtaagcctat ccctaaccct ctcctcggtc tcgattctac gtagtaatga gctagcgcta 1381 accggtggcg cgttaagtcg acaatcaacc tctggatta