Construct: ORF ccsbBroad304_15995
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015897.1_s304c1, BRDN0000409072
- Derived from:
- ccsbBroadEn_15995
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MRM1 (79922)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_15995
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79922 | MRM1 | mitochondrial rRNA methyltr... | NM_024864.5 | 43.9% | 43.9% | 1_594del |
| 2 | human | 79922 | MRM1 | mitochondrial rRNA methyltr... | XM_017025112.2 | 34% | 30.1% | (many diffs) |
| 3 | human | 79922 | MRM1 | mitochondrial rRNA methyltr... | XM_011525275.3 | 32.8% | 28.3% | (many diffs) |
| 4 | human | 79922 | MRM1 | mitochondrial rRNA methyltr... | XM_011525276.3 | 32.8% | 28.3% | (many diffs) |
| 5 | human | 79922 | MRM1 | mitochondrial rRNA methyltr... | XR_934554.2 | 14.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 531
- ORF length:
- 465
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgga cgtgttctcc actgatgacc tcaccggatt tttacagacc aaagcccagc 121 agggctggct cgtggccggc acggtgggct gcccaagcac agaggatccc cagtcctccg 181 agatccccat catgagttgc ttggagttcc tctgggaacg gcctactctc cttgtgctgg 241 ggaatgaggg ctcaggtcta tcccaggagg tgcaggcctc ctgccagctt ctcctcacca 301 tcctgccccg gcgccagctg cctcctggac ttgagtcctt gaacgtctct gtggctgcag 361 gaattcttct tcactccatt tgcagccaga ggaagggttt ccccacagag ggggagagaa 421 ggcagcttct ccaagacccc caagaaccct cagccaggtc tgaagggctc agcatggctc 481 agcacccagg gctgtcttca ggcccagaga aagagaggca aaatgagggc tnnccaactt 541 tcttgtacaa agtggttggt aagcctatcc ctaaccctct cctcggtctc gattctacgt 601 agtaatgagc tagcgctaac cggtggcgcg ttaagtcgac aatcaacctc tggatta