Construct: ORF ccsbBroad304_16014
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014816.1_s304c1, BRDN0000402054
- Derived from:
- ccsbBroadEn_16014
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ADAMTS12 (81792)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_16014
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 81792 | ADAMTS12 | ADAM metallopeptidase with ... | NM_001324511.2 | 98.7% | 98.7% | 688_696delTTGTTTTAT |
| 2 | human | 81792 | ADAMTS12 | ADAM metallopeptidase with ... | NM_001324512.2 | 14.6% | 14.2% | (many diffs) |
| 3 | human | 81792 | ADAMTS12 | ADAM metallopeptidase with ... | NM_030955.4 | 13.8% | 13.4% | (many diffs) |
| 4 | human | 81792 | ADAMTS12 | ADAM metallopeptidase with ... | XM_017009905.1 | 13.5% | 13.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 756
- ORF length:
- 687
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gccatgtgcc cagaggagct ggcttgcaaa cctttccgtg gtggctcagc 121 tccttaactt tggggcgctt tgctatggga gacagcctca gccaggcccg gttcgcttcc 181 cggacaggag gcaagagcat tttatcaagg gcctgccaga ataccacgtg gtgggtccag 241 tccgagtaga tgccagtggg cattttttgt catatggctt gcactatccc atcacgagca 301 gcaggaggaa gagagatttg gatggctcag aggactgggt gtactacaga atttctcacg 361 aggagaagga cctgtttttt aacttgacgg tcaatcaagg atttctttcc aatagctaca 421 tcatggagaa gagatatggg aacctctccc atgttaagat gatggcttcc tctgcccccc 481 tctgccatct cagtggcacg gttctacagc agggcaccag agttgggacg gcagccctca 541 gtgcctgcca tggactgact ggatttttcc aactaccaca tggagacttt ttcattgaac 601 ccgtgaagaa gcatccactg gttgagggag ggtaccaccc gcacatcgtt tacaggaggc 661 agaaagttcc agaaaccaag gagccaacct gtggattaaa gggtattgtg actcacatgt 721 cctcctgggt tgaagaatct gttttgttct tttggtnncc aactttcttg tacaaagtgg 781 ttggtaagcc tatccctaac cctctcctcg gtctcgattc tacgtagtaa tgagctagcg 841 ctaaccggtg gcgcgttaag tcgacaatca acctctggat ta