Construct: ORF ccsbBroad304_16032
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004059.1_s304c1, BRDN0000401223
- Derived from:
- ccsbBroadEn_16032
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MCRIP2 (84331)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_16032
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84331 | MCRIP2 | MAPK regulated corepressor ... | NM_001331229.2 | 53% | 51.5% | (many diffs) |
| 2 | human | 84331 | MCRIP2 | MAPK regulated corepressor ... | NM_138418.4 | 46.8% | 40.6% | 181_308del;354_480del |
| 3 | human | 84331 | MCRIP2 | MAPK regulated corepressor ... | NR_138606.2 | 28% | 1_164del;390_803del | |
| 4 | human | 84331 | MCRIP2 | MAPK regulated corepressor ... | NR_138608.2 | 21.1% | 1_164del;345_605del;651_1064del | |
| 5 | human | 84331 | MCRIP2 | MAPK regulated corepressor ... | NR_138604.2 | 21% | 1_164del;345_609del;655_1068del | |
| 6 | human | 286467 | FIRRE | firre intergenic repeating ... | NR_026975.2 | 6.5% | (many diffs) | |
| 7 | mouse | 68241 | Mcrip2 | MAPK regulated corepressor ... | NM_026633.3 | 40.4% | 30.1% | (many diffs) |
| 8 | mouse | 68241 | Mcrip2 | MAPK regulated corepressor ... | XM_006524838.2 | 36.7% | 27.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 291
- ORF length:
- 225
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgta caccatcacc aaggggccca gcaagctggt cgcgcagcgc cgcacaggtc 121 ccacgcagca gcaggtggag ggccggctcg gcgagctcct gaaatgccgg cagcccgcgc 181 cgccgacctc gcagcccccg cgggcgcagc cctttgcgca gccgccggga ccctggcccc 241 tgtcgagcct ggcagcaggt gcaacagcag ctggatggtg gcccagccgg tnnccaactt 301 tcttgtacaa agtggttggt aagcctatcc ctaaccctct cctcggtctc gattctacgt 361 agtaatgagc tagcgctaac cggtggcgcg ttaagtcgac aatcaacctc tggatta