Construct: ORF ccsbBroad304_16099
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000321.1_s304c1, BRDN0000405925
- Derived from:
- ccsbBroadEn_16099
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ABHD12B (145447)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_16099
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 145447 | ABHD12B | abhydrolase domain containi... | NM_181533.3 | 44.7% | 44.7% | 1_423del |
| 2 | human | 145447 | ABHD12B | abhydrolase domain containi... | NM_181814.1 | 40% | 40% | 1_513del |
| 3 | human | 145447 | ABHD12B | abhydrolase domain containi... | XM_011536474.2 | 35.6% | 35.6% | 1_618del |
| 4 | human | 145447 | ABHD12B | abhydrolase domain containi... | NM_001206673.2 | 31.4% | 31.4% | 1_744del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 408
- ORF length:
- 342
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgtg ggttgcaagt atcaattatc ccttgttaaa gatttaccgg aacattccag 121 gatttttacg tacacttatg gatgccctga gaaaagacaa aataatcttt cctaatgatg 181 aaaatgttaa attcctttct tctcctcttc tcatcttaca tggagaggat gacaggacag 241 tgcctttgga gtatgggaaa aagctctatg aaattgcacg caatgcatac aggaacaaag 301 agagggtcaa gatggttatc tttcctcctg gcttccaaca caacctgctt tgtaaaagcc 361 ccacactgtt aataaccgtg agagatttcc tgagcaagca gtggtcatnn ccaactttct 421 tgtacaaagt ggttggtaag cctatcccta accctctcct cggtctcgat tctacgtagt 481 aatgagctag cgctaaccgg tggcgcgtta agtcgacaat caacctctgg atta