Transcript: Human NM_181533.3

Homo sapiens abhydrolase domain containing 12B (ABHD12B), transcript variant 2, mRNA.

Source:
NCBI, updated 2018-06-23
Taxon:
Homo sapiens (human)
Gene:
ABHD12B (145447)
Length:
1580
CDS:
209..976

Additional Resources:

NCBI RefSeq record:
NM_181533.3
NBCI Gene record:
ABHD12B (145447)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_181533.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000075302 GCTCTATGAAATTGCACGCAA pLKO.1 829 CDS 100% 2.640 2.112 N ABHD12B n/a
2 TRCN0000075299 CGTGATGGGAACCCAATTATT pLKO.1 293 CDS 100% 15.000 10.500 N ABHD12B n/a
3 TRCN0000427584 AGTTGCTATGGTCCGAATATC pLKO_005 1289 3UTR 100% 13.200 9.240 N ABHD12B n/a
4 TRCN0000075298 GCCATTGACTTCTCTACAAAT pLKO.1 1081 3UTR 100% 13.200 9.240 N ABHD12B n/a
5 TRCN0000414904 GGTACAGGAGTTGCAACAAAT pLKO_005 542 CDS 100% 13.200 9.240 N ABHD12B n/a
6 TRCN0000424843 GTGGGTTGCAAGTATCAATTA pLKO_005 634 CDS 100% 13.200 9.240 N ABHD12B n/a
7 TRCN0000075300 CCCAGTTGATGCTATTGTCTT pLKO.1 592 CDS 100% 4.950 3.465 N ABHD12B n/a
8 TRCN0000075301 GCCCAGTTGATGCTATTGTCT pLKO.1 591 CDS 100% 3.000 1.800 N ABHD12B n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_181533.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_09620 pDONR223 100% 88.7% 87.7% None (many diffs) n/a
2 ccsbBroad304_09620 pLX_304 0% 88.7% 87.7% V5 (many diffs) n/a
3 TRCN0000475071 CAGACGAAAGCTCACAATCTTGGC pLX_317 66.7% 88.7% 87.7% V5 (many diffs) n/a
4 ccsbBroadEn_16099 pDONR223 0% 44.7% 44.7% None 1_423del n/a
5 ccsbBroad304_16099 pLX_304 0% 44.7% 44.7% V5 1_423del n/a
6 TRCN0000472985 TTTAAGATTTCTGGGCTTCCCCCT pLX_317 100% 44.7% 44.7% V5 1_423del n/a
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