Construct: ORF ccsbBroad304_16129
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011070.1_s304c1, BRDN0000400454
- Derived from:
- ccsbBroadEn_16129
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ALKBH3 (221120)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_16129
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 221120 | ALKBH3 | alkB homolog 3, alpha-ketog... | NM_139178.4 | 48.4% | 48.6% | 1_441del;726G>T |
| 2 | mouse | 69113 | Alkbh3 | alkB homolog 3, alpha-ketog... | XM_017319248.1 | 71.4% | 76.7% | (many diffs) |
| 3 | mouse | 69113 | Alkbh3 | alkB homolog 3, alpha-ketog... | NM_026944.1 | 42% | 45.1% | (many diffs) |
| 4 | mouse | 69113 | Alkbh3 | alkB homolog 3, alpha-ketog... | XM_006500123.3 | 42% | 45.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 483
- ORF length:
- 417
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgga accaaatcct cactggcacc ctgtgctgcg cacactaaag aaccgcattg 121 aagagaacac tggccacacc ttcaactcct tactctgcaa tctttatcgc aatgagaagg 181 acagcgtgga ctggcacagt gatgatgaac cctcactagg gaggtgcccc attattgctt 241 cactaagttt tggtgccaca cgcacatttg agatgagaaa gaagccacca ccagaagaga 301 atggagacta cacatatgtg gaaagagtga agataccctt ggatcatggt accttgttaa 361 tcatggaagg agcgacacaa gctgactggc agcatcgagt gcccaaagaa taccactcta 421 gagaaccgag agtgaacctg acctttcgga cagtctatcc agaccctcga ggggcaccct 481 ggtnnccaac tttcttgtac aaagtggttg gtaagcctat ccctaaccct ctcctcggtc 541 tcgattctac gtagtaatga gctagcgcta accggtggcg cgttaagtcg acaatcaacc 601 tctggatta