Construct: ORF ccsbBroad304_16148
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012504.1_s304c1, BRDN0000411229
- Derived from:
- ccsbBroadEn_16148
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EPHA10 (284656)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_16148
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 284656 | EPHA10 | EPH receptor A10 | NM_173641.2 | 100% | 100% | |
| 2 | human | 284656 | EPHA10 | EPH receptor A10 | NM_001099439.2 | 29% | 28% | (many diffs) |
| 3 | mouse | 230735 | Epha10 | Eph receptor A10 | NM_177671.5 | 87.8% | 92.8% | (many diffs) |
| 4 | mouse | 230735 | Epha10 | Eph receptor A10 | XM_006503028.3 | 42.5% | 43% | (many diffs) |
| 5 | mouse | 230735 | Epha10 | Eph receptor A10 | XR_376312.1 | 39.3% | (many diffs) | |
| 6 | mouse | 230735 | Epha10 | Eph receptor A10 | XM_006503027.1 | 39.1% | 39.6% | (many diffs) |
| 7 | mouse | 230735 | Epha10 | Eph receptor A10 | XR_867853.1 | 36.3% | (many diffs) | |
| 8 | mouse | 230735 | Epha10 | Eph receptor A10 | XR_001784129.1 | 31.8% | (many diffs) | |
| 9 | mouse | 230735 | Epha10 | Eph receptor A10 | XR_001784128.1 | 26.6% | (many diffs) | |
| 10 | mouse | 230735 | Epha10 | Eph receptor A10 | NM_001256432.1 | 26% | 26.4% | (many diffs) |
| 11 | mouse | 230735 | Epha10 | Eph receptor A10 | XM_017320152.1 | 11% | 10.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 951
- ORF length:
- 885
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgga gacctgcgcc ggtccacacc cgctgcgcct cttcctctgc cggatgcagc 121 tctgtctcgc gctgcttttg ggaccctggc ggcctgggac cgccgaggaa gttatcctcc 181 tggattccaa agcctcccag gccgagctgg gctggactgc actgccaagt aatgggtggg 241 aggagatcag cggcgtggat gaacacgacc gtcccatccg cacgtaccaa gtgtgcaatg 301 tgctggagcc caaccaggac aactggctgc agactggctg gataagccgt ggccgcgggc 361 agcgcatctt cgtggaactg cagttcacac tccgtgactg cagcagcatc cctggcgccg 421 cgggtacctg caaggagacc ttcaacgtct actacctgga aactgaggcc gacctgggcc 481 gtgggcgtcc ccgcctaggc ggcagccggc cccgcaaaat cgacacgatc gcggcggacg 541 agagcttcac gcagggcgac ctgggtgagc gcaagatgaa gctgaacaca gaggtgcgcg 601 agatcggacc gctcagccgg cggggtttcc acctggcctt tcaggacgtg ggcgcatgcg 661 tggcgcttgt ctcggtgcgc gtctactaca agcagtgccg cgccaccgtg cggggcctgg 721 ccacgttccc agccaccgca gccgagagcg ccttctccac actggtggaa gtggccggaa 781 cgtgcgtggc gcactcggaa ggggagcctg gcagcccccc acgcatgcac tgcggcgccg 841 acggcgagtg gctggtgcct gtgggccgct gcagctgcag cgcgggattc caggagcgtg 901 gtgacttctg cgaaggtatc cagttggctg ggggccgtgg ggtgggagtg tnnccaactt 961 tcttgtacaa agtggttggt aagcctatcc ctaaccctct cctcggtctc gattctacgt 1021 agtaatgagc tagcgctaac cggtggcgcg ttaagtcgac aatcaacctc tggatta