Construct: ORF ccsbBroad304_16155
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005945.1_s304c1, BRDN0000412061
- Derived from:
- ccsbBroadEn_16155
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KRTAP19-4 (337971)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_16155
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 337971 | KRTAP19-4 | keratin associated protein ... | NM_181610.3 | 100% | 100% | |
| 2 | human | 337974 | KRTAP19-7 | keratin associated protein ... | NM_181614.2 | 64.1% | 57.4% | (many diffs) |
| 3 | human | 337973 | KRTAP19-6 | keratin associated protein ... | NM_181612.3 | 60.7% | 53.5% | (many diffs) |
| 4 | human | 337972 | KRTAP19-5 | keratin associated protein ... | NM_181611.3 | 58% | 54.8% | (many diffs) |
| 5 | human | 337970 | KRTAP19-3 | keratin associated protein ... | NM_181609.4 | 56.6% | 52.9% | (many diffs) |
| 6 | mouse | 170654 | Krtap19-4 | keratin associated protein ... | NM_130873.1 | 51.1% | 49.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 321
- ORF length:
- 252
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gagctactat ggcagctatt acagaggcct gggctatggc tgtggaggct 121 ttggtggcct aggctatggc tatggctgtg gatgtggcag cttccgcaga ctgggttatg 181 gctgtggctt tggaggcaac ggatatggct actgccgccc atcatgctat ggaggatatg 241 gattctcaat tctactgaaa tcctaccctg aggacacgat atcagaggtc ataagaagat 301 cattcaattt aaccaaatat tnnccaactt tcttgtacaa agtggttggt aagcctatcc 361 ctaaccctct cctcggtctc gattctacgt agtaatgagc tagcgctaac cggtggcgcg 421 ttaagtcgac aatcaacctc tggatta