Construct: ORF ccsbBroad304_16168
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016978.1_s304c1, BRDN0000408436
- Derived from:
- ccsbBroadEn_16168
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FAM74A4 (401508)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_16168
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 653123 | FAM74A6 | family with sequence simila... | NR_110999.1 | 47.6% | 1_179del;549_775del | |
| 2 | human | 401507 | FAM74A1 | family with sequence simila... | NR_026803.2 | 43.4% | (many diffs) | |
| 3 | human | 401508 | FAM74A4 | family with sequence simila... | NR_026802.1 | 19.8% | 1_325del;695_1860del | |
| 4 | human | 728495 | FAM74A3 | family with sequence simila... | NR_026801.1 | 19.4% | (many diffs) | |
| 5 | human | 100996582 | FAM74A7 | family with sequence simila... | NR_126166.1 | 19.1% | (many diffs) | |
| 6 | human | 401508 | FAM74A4 | family with sequence simila... | NR_110998.1 | 18.9% | 1_325del;695_1944del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 438
- ORF length:
- 369
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gtggagggag ctcagaggct gtcctggagg agacgtggag acagctcaga 121 ggctgtccca gagaagacgt ggaaagagct cagaggctgt cccagagaag acgtggagag 181 ctcagaggat gtcccagaga agacgtggag agagttcaga ggctgtcccg gagaagacgt 241 ggaaagagct cagaaactca gagactgtcc cggagaagac atggaaacag ctcagaggct 301 gtctgcaaga agacgtgcag agagttcaga ggctgtcccg gagaagacat ggagagagct 361 caaaggctgt ccacaagaag atgtggagag agttcagagg ctgtcgttac tgctgcactt 421 ggctgttttt ctttggatnn ccaactttct tgtacaaagt ggttggtaag cctatcccta 481 accctctcct cggtctcgat tctacgtagt aatgagctag cgctaaccgg tggcgcgtta 541 agtcgacaat caacctctgg atta