Construct: ORF ccsbBroad304_16169
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008664.1_s304c1, BRDN0000405770
- Derived from:
- ccsbBroadEn_16169
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MZT2A (653784)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_16169
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 653784 | MZT2A | mitotic spindle organizing ... | NM_001085365.2 | 76.5% | 76.5% | 1_111del |
| 2 | human | 80097 | MZT2B | mitotic spindle organizing ... | NM_025029.5 | 74.6% | 72.7% | (many diffs) |
| 3 | human | 653784 | MZT2A | mitotic spindle organizing ... | XM_024453048.1 | 57.8% | 57.8% | 1_264del |
| 4 | human | 653784 | MZT2A | mitotic spindle organizing ... | XM_024453049.1 | 57.8% | 57.8% | 1_264del |
| 5 | human | 80097 | MZT2B | mitotic spindle organizing ... | NM_001330282.2 | 54.1% | 52.7% | (many diffs) |
| 6 | human | 653784 | MZT2A | mitotic spindle organizing ... | XM_024453051.1 | 44.9% | 44.9% | 1_264del;471_650del |
| 7 | human | 653784 | MZT2A | mitotic spindle organizing ... | XM_005263742.3 | 41.9% | 34.4% | (many diffs) |
| 8 | human | 653784 | MZT2A | mitotic spindle organizing ... | XM_017004744.2 | 41.6% | 34.7% | (many diffs) |
| 9 | human | 653784 | MZT2A | mitotic spindle organizing ... | XM_024453050.1 | 41.6% | 34.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 429
- ORF length:
- 363
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgga gctgtacgag ctggctcagg cggcgggcgg cggtatcgac cccgacgtgt 121 tcaagatcct ggtggacctg ctgaagctga acgtggcccc cctcgccgtc ttccagatgc 181 tcaagtccat gtgtgccggg cagaggctag cgagcgagcc ccaggaccct gcggccgtgt 241 ctctgcccac gtcgagcgtg cccgagaccc gagggagaga caaaggcagc gctgccctcg 301 ggggagtatt ggccctggcg gaacgcagca accacgaggg atccagccag aggatgccac 361 gccagcccag cgctaccagg ctgcccaagg ggggcgggcc tgggaagagc cctacgcagg 421 gcagcacctn nccaactttc ttgtacaaag tggttggtaa gcctatccct aaccctctcc 481 tcggtctcga ttctacgtag taatgagcta gcgctaaccg gtggcgcgtt aagtcgacaa 541 tcaacctctg gatta