Construct: ORF ccsbBroad304_16174
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019062.1_s304c1, BRDN0000464783
- Derived from:
- ccsbBroadEn_16174
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- HRAS (3265)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_16174
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3265 | HRAS | HRas proto-oncogene, GTPase | NM_005343.2:c.35G>T | 100% | 100% | |
| 2 | human | 3265 | HRAS | HRas proto-oncogene, GTPase | NM_001130442.2 | 99.8% | 99.4% | 35G>T |
| 3 | human | 3265 | HRAS | HRas proto-oncogene, GTPase | NM_005343.4 | 99.8% | 99.4% | 35G>T |
| 4 | human | 3265 | HRAS | HRas proto-oncogene, GTPase | NM_176795.4 | 85.1% | 79.8% | (many diffs) |
| 5 | human | 3265 | HRAS | HRas proto-oncogene, GTPase | NM_001318054.2 | 38.2% | 26.9% | 0_1ins319;132_213del |
| 6 | mouse | 15461 | Hras | Harvey rat sarcoma virus on... | NM_001130443.1 | 87.4% | 99.4% | (many diffs) |
| 7 | mouse | 15461 | Hras | Harvey rat sarcoma virus on... | NM_008284.2 | 87.4% | 99.4% | (many diffs) |
| 8 | mouse | 15461 | Hras | Harvey rat sarcoma virus on... | XM_006536159.2 | 87.4% | 99.4% | (many diffs) |
| 9 | mouse | 15461 | Hras | Harvey rat sarcoma virus on... | NM_001130444.1 | 74.8% | 78.8% | (many diffs) |
| 10 | mouse | 15461 | Hras | Harvey rat sarcoma virus on... | XR_389950.2 | 44.4% | (many diffs) | |
| 11 | mouse | 15461 | Hras | Harvey rat sarcoma virus on... | XR_389951.2 | 43.1% | (many diffs) | |
| 12 | mouse | 15461 | Hras | Harvey rat sarcoma virus on... | XR_389952.2 | 40.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 636
- ORF length:
- 567
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gacggaatat aagctggtgg tggtgggcgc cgtcggtgtg ggcaagagtg 121 cgctgaccat ccagctgatc cagaaccatt ttgtggacga atacgacccc actatagagg 181 attcctaccg gaagcaggtg gtcattgatg gggagacgtg cctgttggac atcctggata 241 ccgccggcca ggaggagtac agcgccatgc gggaccagta catgcgcacc ggggagggct 301 tcctgtgtgt gtttgccatc aacaacacca agtcttttga ggacatccac cagtacaggg 361 agcagatcaa acgggtgaag gactcggatg acgtgcccat ggtgctggtg gggaacaagt 421 gtgacctggc tgcacgcact gtggaatctc ggcaggctca ggacctcgcc cgaagctacg 481 gcatccccta catcgagacc tcggccaaga cccggcaggg agtggaggat gccttctaca 541 cgttggtgcg tgagatccgg cagcacaagc tgcggaagct gaaccctcct gatgagagtg 601 gccccggctg catgagctgc aagtgtgtgc tctcctgacc aactttcttg tacaaagtgg 661 ttggtaagcc tatccctaac cctctcctcg gtctcgattc tacgtagtaa tgagctagcg 721 ctaaccggtg gcgcgttaag tcgacaatca acctctggat ta