Construct: ORF ccsbBroadEn_00193
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013316.1_s300c1, BRDN0000455195
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TMEM258 (746)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 746 | TMEM258 | transmembrane protein 258 | NM_014206.4 | 100% | 100% | |
| 2 | mouse | 69038 | Tmem258 | transmembrane protein 258 | NM_026919.1 | 91.9% | 100% | (many diffs) |
| 3 | mouse | 69038 | Tmem258 | transmembrane protein 258 | NM_001163431.1 | 86% | 93.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 303
- ORF length:
- 237
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaactttg tacaAAAAAG 61 TTGGCATGGA GCTCGAGGCC ATGAGCAGAT ATACCAGCCC AGTGAACCCA GCTGTCTTCC 121 CCCATCTGAC CGTGGTGCTT TTGGCCATTG GCATGTTCTT CACCGCCTGG TTCTTCGTTT 181 ACGAGGTCAC CTCTACCAAG TACACTCGTG ATATCTATAA AGAGCTCCTC ATCTCCTTAG 241 TGGCCTCACT CTTCATGGGC TTTGGAGTCC TCTTCCTGCT GCTCTGGGTT GGCATCTACG 301 TGTGCCCAAC TTTCTTGTAC AAAGTTGGca ttataagaaa gcattgctta tcaatttgtt 361 gcaacgaac