Construct: ORF ccsbBroadEn_00321

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF012872.1_s300c1, BRDN0000387781
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
CLCN4 (1183)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 1183 CLCN4 chloride voltage-gated chan... NM_001830.4 100% 100%
2 human 1183 CLCN4 chloride voltage-gated chan... NM_001256944.1 87.6% 87.6% 0_1ins282
3 mouse 12727 Clcn4 chloride channel, voltage-s... NM_011334.4 86.7% 96.8% (many diffs)
4 mouse 12727 Clcn4 chloride channel, voltage-s... XM_006539501.3 86.7% 96.8% (many diffs)
5 mouse 12727 Clcn4 chloride channel, voltage-s... XM_006539502.3 86.7% 96.8% (many diffs)
6 mouse 12727 Clcn4 chloride channel, voltage-s... XM_011250426.2 86.7% 96.8% (many diffs)
7 mouse 12727 Clcn4 chloride channel, voltage-s... XM_006539503.3 83.7% 91.6% (many diffs)
8 mouse 12727 Clcn4 chloride channel, voltage-s... XM_006539504.3 83.3% 93% (many diffs)
9 mouse 12727 Clcn4 chloride channel, voltage-s... NM_001302387.1 83% 92.8% (many diffs)
10 mouse 12727 Clcn4 chloride channel, voltage-s... XM_017321961.1 83% 92.8% (many diffs)
11 mouse 12727 Clcn4 chloride channel, voltage-s... NM_001302386.1 79.6% 89% (many diffs)
12 mouse 12727 Clcn4 chloride channel, voltage-s... XM_006539507.3 77.1% 86.3% (many diffs)
13 mouse 12727 Clcn4 chloride channel, voltage-s... XR_391040.3 35.5% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
2349
ORF length:
2280
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaacttTG TACAAAAAAG
61TTGGCACCAT GGTCAATGCG GGAGCGATGA GTGGCTCTGG AAACCTGATG GATTTCCTCG
121ATGAGCCGTT CCCTGATGTG GGGACGTATG AGGACTTCCA CACCATCGAC TGGCTAAGGG
181AAAAGTCACG GGACACCGAC AGACACAGGA AGATCACCAG CAAGAGCAAG GAGTCCATAT
241GGGAGTTCAT CAAGAGCCTG CTGGATGCCT GGTCGGGATG GGTGGTGATG CTGCTCATCG
301GCCTGCTGGC GGGCACCTTG GCTGGGGTCA TCGATCTCGC CGTGGACTGG ATGACGGACC
361TGAAGGAGGG GGTCTGCCTG TCTGCCTTCT GGTATAGCCA TGAGCAGTGT TGCTGGACTT
421CTAACGAGAC CACTTTTGAG GACAGAGACA AGTGTCCCCT GTGGCAGAAA TGGTCGGAGC
481TGCTGGTGAA TCAGTCAGAG GGTGCCAGTG CTTACATTCT GAATTACTTA ATGTACATCC
541TATGGGCGCT GCTGTTTGCA TTTTTGGCTG TCTCCCTGGT GCGTGTATTT GCACCATATG
601CCTGTGGCTC TGGCATACCA GAGATAAAGA CCATTTTGAG CGGCTTTATC ATCAGGGGCT
661ACTTGGGGAA GTGGACCCTG CTAATCAAGA CAGTCACGCT GGTGCTGGTA GTGTCCTCCG
721GTCTGAGCCT TGGGAAGGAA GGGCCGCTAG TGCACGTGGC TTGTTGCTGT GGCAACTTCT
781TCAGCAGCCT TTTCTCCAAG TACAGCAAGA ATGAGGGCAA GAGGCGGGAG GTGCTTTCAG
841CTGCAGCGGC TGCTGGAGTC TCTGTTGCCT TTGGTGCACC AATTGGAGGC GTGCTTTTCA
901GTCTAGAAGA GGTCAGTTAC TACTTTCCCC TGAAGACCTT GTGGAGGTCA TTTTTCGCAG
961CCCTGGTGGC GGCCTTTACG CTGAGATCCA TCAATCCCTT TGGGAATAGC CGTCTCGTTC
1021TCTTTTATGT GGAATACCAC ACGCCCTGGT ACATGGCTGA ACTCTTCCCC TTCATCCTGC
1081TTGGGGTCTT CGGGGGCTTG TGGGGAACCC TCTTCATCCG CTGCAACATC GCCTGGTGCA
1141GGAGGCGCAA GACCACCAGG CTGGGGAAGT ACCCGGTGCT GGAGGTCATT GTGGTGACTG
1201CCATCACTGC CATCATTGCC TACCCCAATC CCTACACACG CCAGAGCACC AGCGAGCTCA
1261TTTCTGAGCT GTTCAATGAC TGTGGAGCCC TTGAGTCTTC CCAGCTCTGT GACTACATCA
1321ATGACCCCAA CATGACTCGG CCTGTGGATG ACATTCCAGA CCGGCCGGCT GGTGTCGGTG
1381TTTACACGGC CATGTGGCAG CTGGCCCTGG CACTGATCTT CAAAATCGTC GTTACCATAT
1441TTACCTTTGG CATGAAGATC CCGTCGGGCC TCTTCATCCC CAGCATGGCT GTGGGCGCGA
1501TAGCGGGCAG GATGGTGGGA ATTGGCGTGG AGCAGCTGGC CTACCATCAC CATGACTGGA
1561TCATCTTCAG GAACTGGTGC AGACCCGGTG CAGACTGTGT CACGCCAGGG CTGTACGCAA
1621TGGTGGGAGC TGCGGCCTGC CTCGGTGGAG TTACCAGGAT GACGGTGTCA TTGGTGGTCA
1681TCATGTTTGA ATTAACCGGG GGTCTGGAGT ACATCGTGCC CCTGATGGCG GCGGCTGTGA
1741CCAGCAAGTG GGTAGCTGAT GCATTTGGGA AAGAAGGCAT CTACGAGGCC CACATCCACT
1801TAAATGGGTA CCCTTTCCTT GACGTGAAGG ACGAGTTTAC TCACCGCACA CTGGCCACCG
1861ACGTCATGCG GCCCCGGCGG GGAGAGCCGC CACTGTCGGT GCTCACCCAG GACAGCATGA
1921CTGTCGAGGA CGTGGAGACG CTCATCAAGG AGACCGACTA CAACGGCTTC CCCGTGGTGG
1981TCTCCAGAGA CTCCGAGCGC CTCATTGGAT TTGCCCAGAG GAGGGAACTG ATTCTCGCAA
2041TAAAGAACGC CAGACAGAGG CAGGAGGGCA TTGTGAGCAA TTCCATCATG TACTTCACGG
2101AGGAACCCCC CGAGCTGCCG GCCAACAGCC CACATCCCCT GAAGCTGCGG CGCATCCTGA
2161ACCTCAGCCC GTTTACAGTG ACAGACCACA CTCCGATGGA AACGGTGGTG GATATCTTCC
2221GGAAACTGGG GCTTCGGCAG TGCCTGGTGA CGCGGAGCGG GAGACTTCTT GGCATCATCA
2281CAAAAAAGGA TGTTCTGAGA CATATGGCCC AGATGGCAAA CCAGGACCCC GAATCCATCA
2341TGTTTAATTT GCCAACTTTC TTGTACAAAG Ttggcattat aagaaagcat tgcttatcaa
2401tttgttgcaa cgaac

Download FASTA (ORF) (Full)