Construct: ORF ccsbBroadEn_00434

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF007748.1_s300c1, BRDN0000390506
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
DEFA1 (1667)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 728358 DEFA1B defensin alpha 1B NM_001042500.1 100% 100%
2 human 1667 DEFA1 defensin alpha 1 NM_004084.3 100% 100%
3 human 1668 DEFA3 defensin alpha 3 NM_005217.4 99.6% 98.9% 194A>C
4 human 728358 DEFA1B defensin alpha 1B NM_001302265.1 93% 93% 1_21del
5 human 1667 DEFA1 defensin alpha 1 XM_011534740.3 93% 93% 1_21del
6 human 1668 DEFA3 defensin alpha 3 XM_011534741.2 92.7% 92% 1_21del;215A>C
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
348
ORF length:
282
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGAG GACCCTCGCC ATCCTTGCTG CCATTCTCCT GGTGGCCCTG CAGGCCCAGG
121CTGAGCCACT CCAGGCAAGA GCTGATGAGG TTGCTGCAGC CCCGGAGCAG ATTGCAGCGG
181ACATCCCAGA AGTGGTTGTT TCCCTTGCAT GGGACGAAAG CTTGGCTCCA AAGCATCCAG
241GCTCAAGGAA AAACATGGCC TGCTATTGCA GAATACCAGC GTGCATTGCA GGAGAACGTC
301GCTATGGAAC CTGCATCTAC CAGGGAAGAC TCTGGGCATT CTGCTGCTGC CCAACTTTCT
361TGTACAAAGT tggcattata agaaagcatt gcttatcaat ttgttgcaac gaac

Download FASTA (ORF) (Full)