Construct: ORF ccsbBroadEn_00524

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF010028.1_s300c1, BRDN0000396876
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
EXT2 (2132)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 2132 EXT2 exostosin glycosyltransfera... NM_207122.1 100% 100%
2 human 2132 EXT2 exostosin glycosyltransfera... XM_011519951.1 98.2% 98.2% 1_39del
3 human 2132 EXT2 exostosin glycosyltransfera... NM_001178083.2 97.3% 96.2% (many diffs)
4 human 2132 EXT2 exostosin glycosyltransfera... NM_000401.3 95.6% 95.6% 1_99del
5 human 2132 EXT2 exostosin glycosyltransfera... XM_011519950.1 93.9% 93.9% 1_138del
6 human 2132 EXT2 exostosin glycosyltransfera... XM_024448383.1 57.9% 57.3% (many diffs)
7 mouse 14043 Ext2 exostoses (multiple) 2 NM_010163.3 87.8% 95.4% (many diffs)
8 mouse 14043 Ext2 exostoses (multiple) 2 XM_006498733.3 87.8% 95.4% (many diffs)
9 mouse 14043 Ext2 exostoses (multiple) 2 XM_006498732.3 86% 93.4% (many diffs)
10 mouse 14043 Ext2 exostoses (multiple) 2 XM_006498731.3 84.3% 91.5% (many diffs)
11 mouse 14043 Ext2 exostoses (multiple) 2 XM_006498730.3 81.6% 88.6% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2220
ORF length:
2154
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGTG TGCGTCGGTC AAGTATAATA TCCGGGGTCC TGCCCTCATC CCAAGAATGA
121AGACCAAGCA CCGAATCTAC TATATCACCC TCTTCTCCAT TGTCCTCCTG GGCCTCATTG
181CCACTGGCAT GTTTCAGTTT TGGCCCCATT CTATCGAGTC CTCAAATGAC TGGAATGTAG
241AGAAGCGCAG CATCCGTGAT GTGCCGGTTG TTAGGCTGCC AGCCGACAGT CCCATCCCAG
301AGCGGGGGGA TCTCAGTTGC AGAATGCACA CGTGTTTTGA TGTCTATCGC TGTGGCTTCA
361ACCCAAAGAA CAAAATCAAG GTGTATATCT ATGCTCTGAA AAAGTACGTG GATGACTTTG
421GCGTCTCTGT CAGCAACACC ATCTCCCGGG AGTATAATGA ACTGCTCATG GCCATCTCAG
481ACAGTGACTA CTACACTGAT GACATCAACC GGGCCTGTCT GTTTGTTCCC TCCATCGATG
541TGCTTAACCA GAACACACTG CGCATCAAGG AGACAGCACA AGCGATGGCC CAGCTCTCTA
601GGTGGGATCG AGGTACGAAT CACCTGTTGT TCAACATGTT GCCTGGAGGT CCCCCAGATT
661ATAACACAGC CCTGGATGTC CCCAGAGACA GGGCCCTGTT GGCTGGTGGC GGCTTTTCTA
721CGTGGACTTA CCGGCAAGGC TACGATGTCA GCATTCCTGT CTATAGTCCA CTGTCAGCTG
781AGGTGGATCT TCCAGAGAAA GGACCAGGTC CACGGCAATA CTTCCTCCTG TCATCTCAGG
841TGGGTCTCCA TCCTGAGTAC AGAGAGGACC TAGAAGCCCT CCAGGTCAAA CATGGAGAGT
901CAGTGTTAGT ACTCGATAAA TGCACCAACC TCTCAGAGGG TGTCCTTTCT GTCCGTAAGC
961GCTGCCACAA GCACCAGGTC TTCGATTACC CACAGGTGCT ACAGGAGGCT ACTTTCTGTG
1021TGGTTCTTCG TGGAGCTCGG CTGGGCCAGG CAGTATTGAG CGATGTGTTA CAAGCTGGCT
1081GTGTCCCGGT TGTCATTGCA GACTCCTATA TTTTGCCTTT CTCTGAAGTT CTTGACTGGA
1141AGAGAGCATC TGTGGTTGTA CCAGAAGAAA AGATGTCAGA TGTGTACAGT ATTTTGCAGA
1201GCATCCCCCA AAGACAGATT GAAGAAATGC AGAGACAGGC CCGGTGGTTC TGGGAAGCGT
1261ACTTCCAGTC AATTAAAGCC ATTGCCCTGG CCACCCTGCA GATTATCAAT GACCGGATCT
1321ATCCATATGC TGCCATCTCC TATGAAGAAT GGAATGACCC TCCTGCTGTG AAGTGGGGCA
1381GCGTGAGCAA TCCACTCTTC CTCCCGCTGA TCCCACCACA GTCTCAAGGG TTCACCGCCA
1441TAGTCCTCAC CTACGACCGA GTAGAGAGCC TCTTCCGGGT CATCACTGAA GTGTCCAAGG
1501TGCCCAGTCT ATCCAAACTA CTTGTCGTCT GGAATAATCA GAATAAAAAC CCTCCAGAAG
1561ATTCTCTCTG GCCCAAAATC CGGGTTCCAT TAAAAGTTGT GAGGACTGCT GAAAACAAGT
1621TAAGTAACCG TTTCTTCCCT TATGATGAAA TCGAGACAGA AGCTGTTCTG GCCATTGATG
1681ATGATATCAT TATGCTGACC TCTGACGAGC TGCAATTTGG TTATGAGGTC TGGCGGGAAT
1741TTCCTGACCG GTTGGTGGGT TACCCGGGTC GTCTGCATCT CTGGGACCAT GAGATGAATA
1801AGTGGAAGTA TGAGTCTGAG TGGACGAATG AAGTGTCCAT GGTGCTCACT GGGGCAGCTT
1861TTTATCACAA GTATTTTAAT TACCTGTATA CCTACAAAAT GCCTGGGGAT ATCAAGAACT
1921GGGTAGATGC TCATATGAAC TGTGAAGATA TTGCCATGAA CTTCCTGGTG GCCAACGTCA
1981CGGGAAAAGC AGTTATCAAG GTAACCCCAC GAAAGAAATT CAAGTGTCCT GAGTGCACAG
2041CCATAGATGG GCTTTCACTA GACCAAACAC ACATGGTGGA GAGGTCAGAG TGCATCAACA
2101AGTTTGCTTC AGTCTTCGGG ACCATGCCTC TCAAGGTGGT GGAACACCGA GCTGACCCTG
2161TCCTGTACAA AGATGACTTT CCTGAGAAGC TGAAGAGCTT CCCCAACATT GGCAGCTTAT
2221GCCCAACTTT CTTGTACAAA GTtggcatta taagaaagca ttgcttatca atttgttgca
2281acgaac

Download FASTA (ORF) (Full)

GPP Web Portal Terms of Service

Effective Date: December 8, 2025
By using this site, you agree to our terms and conditions below.

Overview of Terms

The data made available on this website were generated for research purposes and are not intended for clinical or commercial uses. Commercial use (or other use for profit-making purposes) of the GPP Web Portal and its tools, is not permitted under these terms and may require a separate license agreement from Broad or its contributors. For more information, please contact partnering@broadinstitute.org.

The original data may be subject to rights claimed by third parties, including but not limited to, patent, copyright, other intellectual property rights, biodiversity-related access and benefit-sharing rights. It is the responsibility of users of Broad Institute services to ensure that their use of the data does not infringe any of the rights of such third parties.

Any questions or comments concerning these Terms of Use can be addressed to: legal@broadinstitute.org.

By accessing and viewing this GPP Web Portal, you agree to the following terms and conditions:

Attribution

You agree to acknowledge the Broad Institute (e.g., in publications, services or products) for any of your use of its online services, databases or software in accordance with good scientific practice. You agree to use the acknowledgment wording provided for the relevant tools as indicated on the FAQ for each tool.

Updating the Terms of Use

We reserve the right to update these Terms of Use at any time. When alterations are inevitable, we will attempt to give reasonable notice of any changes by placing a notice on our website, but you may wish to check each time you use the website. The date of the most recent revision will appear on this, the "GPP Web Portal Terms of Use" page. If you do not agree to these changes, please do not continue to use our online services. We will also make available an archived copy of the previous Terms of Use for comparison.

Indemnification and Disclaimer of Warranties

You are using this GPP Web Portal at your own risk, and you hereby agree to hold Broad and its contributors and their trustees, directors, officers, employees, and affiliated investigators harmless for any third party claims which may arise from your use of the GPP Web Portal, the tools available therein, or any portion thereof. Further, you agree to indemnify Broad, its contributors, and its and their trustees, directors, officers, employees, affiliated investigators, students, and affiliates for any loss, costs, claims, damages, or other liabilities arising from any unpermitted commercial or profit-making use you make of the GPP Web Portal. The GPP Web Portal is a research tool and is provided "as is". Broad does not represent that the GPP Web Portal is free of errors or bugs or suitable for any particular tasks.

ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE, NONINFRINGEMENT, OR THE ABSENCE OF LATENT OR OTHER DEFECTS ARE DISCLAIMED. IN NO EVENT SHALL BROAD OR ITS CONTRIBUTORS BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES HOWEVER CAUSED AND ON ANY THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT (INCLUDING NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY OUT OF THE USE OF THE GPP WEB PORTAL, EVEN IF ADVISED OF THE POSSIBILITY OF SUCH DAMAGE.

Governing Law

The terms and conditions herein shall be construed, governed, interpreted, and applied in accordance with the internal laws of the Commonwealth of Massachusetts, U.S.A. Furthermore, by accessing, downloading, or using the Database, You consent to the personal jurisdiction of, and venue in, the state and federal courts within Massachusetts with respect to Your download or use of the Database.